ABSTRACT
Prevalence of CMV and toxoplasmosis antibodies were determined among normal newborn infants, suspected congenital neonates and pregnant women. Seropositive rates of CMV and toxoplasmosis were similar. Most of the pregnant women had CMV antibodies. The in-house ELISA for detection of Toxoplasma antibodies was developed and compared with the commercial kit with sensitivity and specificity of 90.47% and 96.74% respectively.
Subject(s)
Antibodies, Protozoan/blood , Antibodies, Viral/blood , Cytomegalovirus Infections/immunology , Cytomegalovirus/immunology , Toxoplasma/immunology , Toxoplasmosis, Congenital/immunology , Animals , Cytomegalovirus Infections/congenital , Enzyme-Linked Immunosorbent Assay , Female , Humans , Infant, Newborn , Pregnancy , Seroepidemiologic Studies , Thailand/epidemiologyABSTRACT
The in vitro activity of drugs, namely dehydroemetine, ornidazole, metronidazole and tinidazole were determined against the locally isolated strains of E. histolytica in Thailand. The test was performed in liquid monophasic medium, i.e. liver marmite serum medium. In all, locally isolated strains from thirty hosts studied, the minimal inhibitory concentration (MIC) for dehydroemetine ranged from 0.125 to 1 microgram/ml, ornidazole ranged from 0.0625 to 0.25 microgram/ml, metronidazole ranged from 0.0625 to 0.125 microgram/ml, and tinidazole ranged from 0.0625 microgram/ml to 0.25 microgram/ml. The MIC of dehydroemetine was significantly different from ornidazole, metronidazole and tinidazole. Metronidazole was superior to that of dehydroemetine but was not significantly different among ornidazole, metronidazole and tinidazole.
Subject(s)
Amebicides/pharmacology , Entamoeba histolytica/drug effects , Animals , Drug Resistance , Emetine/pharmacology , Metronidazole/pharmacology , Ornidazole/pharmacology , Tinidazole/pharmacologyABSTRACT
A comparison of the sensitivity of the wet smear, Papanicolaou's stained smear, and culture in Cystein-Peptone-Liver-Maltose (CPLM) medium for the diagnosis of T. vaginalis infection was undertaken in 1, 197 female subjects, both with and without gynaecological disorders. The wet smear method gave 15.5% positives, culture method, 16.5% positives and Papanicolaou's stained smear 6.7% positives. All positive cases by Papanicolaou's method or wet smear method were positive by the culture method. These findings indicate that the wet smear and culture in CPLM medium were better than the Papanicolaou's method.
