1.
Biokhimiia
; 42(2): 306-10, 1977 Feb.
Article
in Russian
| MEDLINE
| ID: mdl-851554
ABSTRACT
The method of pea ferredoxin (Fd) immobilization has been worked out. Under binding Fd with sepharose 4B activated by cyanogen bromide, a destruction took place in the Fd active center and apoprotein was bound with the matrix. The native immobilized Fd has been obtained when using P-300 biogel as a matrix. The gel was activated by glutaraldehyde and protein was linked with gel through a hexamethylenediamine spacer.