ABSTRACT
The effectiveness of two transplantation methods of human hepatocellular carcinoma cells HepG2 and allogeneic mesenchymal stromal cells of adipose tissue (AT MSCs) into mice was compared in order to select the most effective for liver damage repair. Considerable advantage of cell transplantation into the spleen compared with intraperitoneal administration was shown. It is found that, under similar conditions of transplantation, AT MSCs are detected in liver tissue in smaller quantities than human hepatocellular carcinoma cells HepG2; differences in cell localization of these types of cells in the liver are revealed. A tendency to decrease in the degree of fibrotic changes in liver tissue after transplantation of AT MSCs and to a greater extent after transplantation of AT MSCs, pretreated with interleukin-6, was traced.
Subject(s)
Liver/injuries , Liver/metabolism , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/metabolism , Allografts , Animals , Hep G2 Cells , Heterografts , Humans , Mice , Neoplasm TransplantationABSTRACT
We studied the effects of melatonin on differentiation potential of Ito cells during atypical regeneration of mouse liver under conditions of CCl4-induced fibrosis. The dynamics of fibrosis was traced at the histological level and the effects of melatonin on the differentiation potential of mouse Ito cells were evaluated. Melatonin alleviated fibrotic changes in the liver tissue and reduced differentiation of Ito cells into myofibroblasts under conditions of atypical regeneration of the liver in induced fibrosis. The hepatoprotective role of melatonin was shown.
Subject(s)
Hepatic Stellate Cells/drug effects , Liver Cirrhosis/prevention & control , Liver/drug effects , Melatonin/pharmacology , Myofibroblasts/drug effects , Protective Agents/pharmacology , Actins/genetics , Actins/metabolism , Animals , Biomarkers/metabolism , Carbon Tetrachloride , Cell Differentiation/drug effects , Crosses, Genetic , Female , Gene Expression , Hepatic Stellate Cells/metabolism , Hepatic Stellate Cells/pathology , Liver/metabolism , Liver/pathology , Liver Cirrhosis/chemically induced , Liver Cirrhosis/genetics , Liver Cirrhosis/pathology , Liver Regeneration/drug effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Myofibroblasts/metabolism , Myofibroblasts/pathologyABSTRACT
The influence of femtosecond laser pulses on the proliferative activity of HaCaT keratinocytes and mesenchymal stromal cells (MSC) rats was studied. The growth media was exposed by laser pulses with wavelength 590 nm and duration 30 fs. The dependence of proliferative activity of cells on the dose was showed in the range 6-4299 J/cm2. Proliferative activity was assessed by the number of cells after 1 day after exposure. For both cell cultures obtained similar dose dependence: an increase in cell proliferation (32-54% for HaCaT and 19% for MSK) occurs when using lower doses, while higher doses no changes the rate of proliferation of cells. Conducted physical and chemical analysis found no increase in the concentration of active forms of oxygen in the culture medium. The impact of femtosecond laser pulses has led to the generation in culture medium acoustic oscillations in the range of 0.5 to 6.0 kHz. It is assumed that the increase in proliferative activity of cells, can be caused by mechanical effects of acoustic waves generated in the environment of optical breakdown in the focus of the laser radiation.
