ABSTRACT
Every year different industries generate numerous toxic environmental polluting agents throughout the world. Among the polluting agents, chromium (Cr) toxicity is a great concern nowadays. It is continuously released in soil and water, causing environmental and health problems thereby raising several public health issues in developing countries like Bangladesh. The primary goal of this study was to provide a bioremediation option to reduce toxic hexavalent chromium to a less toxic trivalent form by isolating chromium resistant bacteria from Cr contaminated environments. Bacterial isolates were obtained from seven tannery waste samples collected from Hazaribag and Hemayetpur, Savar, Dhaka. Twenty morphologically distinct colonies were screened, of which six showed the highest resistance. These were designated as A1, A2, B1, F1, K1, and P1. Their maximum tolerance to Cr (VI) was determined through growth assays in varying chromium concentrations up to 8000 mg/L on LB agar media. Strains A2 and B1 exhibited the highest resistances to chromium at 7700 mg/L and 7200 mg/L respectively. Bacterial strains A2 and B1 were identified through several biochemical tests and after PCR analysis finally identified as Bacillus sp. and Micrococcus sp. respectively. Their Cr (VI) reduction capabilities were assessed quantitatively using the diphenylcarbazide colorimetric assay. Both strains exhibit approximately 100% reduction of chromium from 100 mg/L concentration to non-toxic form within 48 h using accurate analytical methods. This study demonstrates the isolation of highly chromium-resistant bacteria from tannery waste that can efficiently bioremediate Cr (VI) pollution, thus providing an eco-friendly and cost-effective bioremediation approach.
ABSTRACT
Thalassemia is one of the most common life-threatening yet preventable congenital hemoglobin disorders especially in South Asian regions like Bangladesh. It has become a rising public health concern for Bangladesh as 6-12% of the population are carriers and many of them are unaware of it. The purpose of the study is to inspect the knowledge and attitude towards thalassemia among the general people of Bangladesh. A cross-sectional survey was conducted in eight administrative regions of Bangladesh between January and October of 2020. A structured questionnaire was designed to collect information about thalassemia and socio-demographic characteristics. Multivariate logistic regression models were used to identify factors associated with knowledge of thalassemia. A p-value <0.05 was considered significant. Of the 1,248 participants, only 47.4% had heard of thalassemia. Half of the participants who heard about the disease had no idea that thalassemia was not a transfusion transmitted disease. Only 49.8% of participant correctly identified consanguineous marriages as an important risk factor. Majority of them knew that marriage between two carriers can lead to a child with thalassemia major. About 72.5% knew that blood tests are a diagnosis method to determine thalassemia. Among the socio-demographic variables, the level of education of the respondents was identified as an independent predictor for knowledge (p<0.05) on thalassemia. For example, graduate (aOR: 24.88; 95% CI: 6.238-99.232) or post-graduate (aOR: 33.18; 95% CI: 7.864-140.001) participants were more aware of thalassemia than non-graduates. However, about 68.2% of the participants showed a positive attitude towards premarital screening of themselves or their family members and 85.3% were willing to donate blood to thalassemia patients. The study shows that there is a need to disseminate the information on thalassemia since the knowledge gap is huge among people. These findings will strengthen the implementation of thalassemia major awareness through educational programs, health counseling, premarital screening and campaigning.
ABSTRACT
BACKGROUND: Dengue, the mosquito borne disease has become a growing public health threat in Bangladesh due to its gradual increasing morbidity and mortality since 2000. In 2019, the country witnessed the worst ever dengue outbreak. The present study was conducted to characterize the socio-economic factors and knowledge, attitude and practice (KAP) status towards dengue among the people of Bangladesh. METHOD: A cross-sectional study was conducted with 1,010 randomly selected respondents from nine different administrative regions of Bangladesh between July and November 2019. A structured questionnaire was used covering socio-demographic characteristics of the participants including their knowledge, awareness, treatment and practices regarding dengue fever. Factors associated with the knowledge and awareness of dengue were investigated separately, using multivariable logistic regression. RESULTS: Although majority (93.8%) of the respondents had heard about dengue, however, they had still misconceptions about Aedes breeding habitat. Around half of the study population (45.7%) had mistaken belief that Aedes can breed in dirty water and 43.1% knew that Aedes mosquito usually bites around sunrise and sunset. Fever indication was found in 36.6% of people which is the most common symptom of dengue. Among the socio-demographic variables, the level of education of the respondents was identified as an independent predictor for both knowledge (p<0.05) and awareness (p<0.05) of dengue. The preventive practice level was moderately less than the knowledge level though there was a significant association (p<0.05) existed between knowledge and preventive practices. Our study noted that TV/Radio is an effective predominant source of information about dengue fever. CONCLUSION: As dengue is emerging in Bangladesh, there is an urgent need to increase health promotion activities through campaigns for eliminating the misconception and considerable knowledge gaps about dengue.
