ABSTRACT
Six monoclonal antibody-producing hybridoma cell lines were generated by fusion of NS-1 myeloma cells with BALB/c immune splenocytes. Monoclonal antibodies (MCA) specific to Machupo virus NP protein were used to study cross-reactivity between pathogenic and nonpathogenic arenaviruses. It was shown that 3140 MCA cross-reacted in IFA with Lassa, Tacaribe, and Tamiami arenaviruses whereas 3101 MCA reacted with Machupo virus alone. It was assumed that these 3101 MCA could be used for differentiation of Machupo virus in IFA.
Subject(s)
Antibodies, Monoclonal/isolation & purification , Arenaviruses, New World/immunology , Animals , Antibodies, Monoclonal/analysis , Antibodies, Monoclonal/immunology , Antibody Specificity/immunology , Antigens, Viral/immunology , Cross Reactions/immunology , Epitopes/immunology , Female , Hybridomas/immunology , Immunization , Mice , Mice, Inbred BALB C , Vero Cells , Virus CultivationSubject(s)
Lassa Fever/drug therapy , Ribavirin/therapeutic use , Animals , Antigens, Viral/analysis , Drug Evaluation, Preclinical , Fluorescent Antibody Technique , Lassa Fever/microbiology , Lassa Fever/pathology , Lassa virus/immunology , Lassa virus/isolation & purification , Macaca fascicularisABSTRACT
Data on the stability of Lassa virus, Josia strain, isolated from man to the effect of physicochemical factors (heating at 50 degrees C, solutions of urea and formalin of various concentrations, UV irradiation) as well as on the time course of this strain reproduction in cell lines of different origins are presented. Recommendations for lowering of reactogenicity of the virus-containing material are given. The experimental results must be taken into consideration in the development and manufacture of diagnostic and therapeutic-prophylactic preparations for Lassa fever.
Subject(s)
Lassa virus/growth & development , Animals , Chemical Phenomena , Chemistry, Physical , Culture Media , Dose-Response Relationship, Drug , Formaldehyde/pharmacology , Lassa virus/drug effects , Lassa virus/radiation effects , Mice , Serial Passage , Temperature , Time Factors , Ultraviolet Rays , Virus Cultivation/methods , Virus ReplicationSubject(s)
Antibodies, Viral/biosynthesis , Antigens, Viral/immunology , Arenaviridae/immunology , Lassa Fever/immunology , Lassa virus/immunology , Animals , Brain/microbiology , Hemagglutination, Viral , Immunoenzyme Techniques , Immunoglobulin M/analysis , Kidney/microbiology , Lassa Fever/microbiology , Liver/microbiology , Mice , Spleen/microbiologyABSTRACT
Pathogenicity for guinea pigs and white mice of various Lassa virus variants: native, having had 1 passage in Vero cell culture and 4 passages in newborn white mouse brain; a virus having gone through 10 passages in Vero cells and 8 mouse brain passages (variant No. 10); a small-plaque clone derived from variant No. 10 by the method of plaque-to-plaque cloning (variant No. 11k), was studied. Both the native virus and variant No. 10 were found to be similarly pathogenic for susceptible laboratory animals, while the small-plaque variant of Lassa virus became non-fatal for guinea pigs and white mice. The decline of pathogenic properties in variant No. 11k was shown not to be associated with the presence of temperature-sensitive mutants or defective interfering particles.
