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Int J Biol Macromol ; 72: 1323-8, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25450551

ABSTRACT

Ultrasound assisted extraction of pectin from waste pomegranate peel was investigated and optimized using Box-Behnken response surface design coupled with numerical optimization technique. The individual and interactive effect of process variables (solid-liquid ratio, pH, extraction time and temperature) on the pectin yield was studied. The experimental data obtained were analyzed by Pareto analysis of variance (ANOVA) and second-order polynomial models were developed using multiple regression analysis. The models developed from the experimental design were predictive and good fit with the experimental data with high coefficient of determination (R(2)) value. The optimal extraction condition was found to be 1:17.52 g/ml of solid-liquid ratio, 1.27 of pH, 28.31 min of extraction time and 61.90 °C of extraction temperature respectively. Under the optimal conditions, experimental yield was very close to the predicted values.


Subject(s)
Lythraceae/chemistry , Pectins/isolation & purification , Ultrasonics/methods , Waste Products/analysis , Analysis of Variance , Models, Theoretical , Regression Analysis , Reproducibility of Results
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