ABSTRACT
By optical microscopy, it has been shown that the addition of La3+ ions induced transformation in the shape of erythrocytes, their aggregation and fusion. After addition of La3+ erythrocytes transform into stomatocytes. It was found that the red cell shape recovered to discoid after addition of EDTA. Neither transformation of shape nor aggregation or fusion of erythrocytes could be detected after their treatment with glutaraldehyde. A possible mechanism and significance of the shape transformation in aggregation of erythrocytes is discussed.
Subject(s)
Cell Aggregation/drug effects , Cell Fusion , Cell Size/drug effects , Erythrocytes/drug effects , Lanthanum/pharmacology , Erythrocytes/cytology , Humans , In Vitro TechniquesABSTRACT
The effect of neuraminidase, trypsin and pronase on the electrophoretic mobility of human erythrocytes and their aggregation induced by La3+ was studied in vitro. The aggregation of intact red cells differs from that of cells treated with neuraminidase and proteolytic enzymes. The above differences are observed in the range of La3+ suspension concentration from 20 to 330 microM. A possible mechanism of aggregation is discussed.
Subject(s)
Erythrocytes/physiology , Lanthanum/metabolism , Neuraminidase/metabolism , Pronase/metabolism , Trypsin/metabolism , Cell Aggregation , Electricity , Electrophoresis , Erythrocytes/metabolism , Humans , In Vitro TechniquesABSTRACT
To evaluate the condition of red cell membranes, a new method is proposed basing on the degree of their aggregation after La ions stimulation. The aggregation of intact red cells differs from that of cells with low ATP, treated with glutaric aldehyde, proteolytic enzymes, in various pH. The above differences are observed in the range of LaCl suspension concentrations from 20 to 330 microM.