Subject(s)
Erythrocytes/physiology , Hemoglobinopathies/genetics , Malaria, Falciparum/genetics , Malaria, Vivax/genetics , Animals , Erythrocytes/parasitology , Hemoglobinopathies/parasitology , Host-Parasite Interactions , Humans , Malaria, Falciparum/blood , Malaria, Falciparum/parasitology , Malaria, Vivax/blood , Malaria, Vivax/parasitology , Plasmodium falciparum/physiology , Plasmodium vivax/physiologyABSTRACT
To date RPMI-1640 has been the best medium for cultivation of Plasmodium falciparum in vitro. In addition to this medium, several alternative media, essentially the ones used in animal and plant tissue culture, were employed for the cultivation of P. falciparum. Only the media rich in glucose content, viz. Nitsch medium and White's medium S-3, supported the parasite multiplication.
Subject(s)
Culture Media , Plasmodium falciparum/growth & development , Animals , Evaluation Studies as Topic , In Vitro Techniques , Parasitology/methodsSubject(s)
Plasmodium vivax/growth & development , Animals , Culture Media , In Vitro Techniques , Liver/chemistryABSTRACT
A number of growth promoting substances used in plant tissue culture were investigated for their effect on cultures of malaria parasites. In all, seven such growth promoting substances were employed. Out of these, only three substances, namely indoleacetic acid, indolebutyric acid and gibberellic acid improved P. falciparum growth in vitro.
Subject(s)
Erythrocytes/parasitology , Growth Substances/pharmacology , Plasmodium falciparum/drug effects , Animals , Cells, Cultured , Culture Media , Plasmodium falciparum/growth & developmentABSTRACT
A modified Rieckmann test was developed for assessing chloroquine sensitivity of Plasmodium vivax strains. This test envisages the evaluation of parasite growth with different concentrations of the drug and its comparison with controls, at the end of a 48-hour experimental period. Using this test, 16 strains were assessed for their chloroquine sensitivity. Twelve strains were found to be sensitive, and 4 were resistant to chloroquine.
