ABSTRACT
Fragments of the RNA polymerase beta-subunit genes from representatives of picoplankton of Lake Baikal were cloned using polymerase chain reaction (PCR), and the resulting recombinant clones were sequenced. An analysis of 93 clones revealed 40 different sequences. A comparison of these nucleotide sequences and the corresponding amino acid sequences deduced with their homologues from databanks confirmed the high conservation of the RNA polymerase beta-subunit region involved in the formation of the enzyme active site.
Subject(s)
DNA-Directed RNA Polymerases/genetics , Phytoplankton/genetics , Amino Acid Sequence , Animals , Base Sequence , Cloning, Molecular , DNA Primers , Humans , Molecular Sequence Data , Phytoplankton/enzymology , Polymerase Chain Reaction , Sequence Homology, Amino Acid , SiberiaABSTRACT
A fragment of cDNA and an intron-containing fragment of the human L19 ribosomal protein (RPL19) gene, and introns of the human ribosomal proteins S26 (RPS26) and L32 (RPL32) genes were cloned and sequenced. The intron-containing genes of these ribosomal proteins were mapped to human chromosomes by means of polymerase chain reaction (PCR) using a human/rodent hybrid DNA panel.
Subject(s)
Chromosomes, Human , Ribosomal Proteins/genetics , Animals , Base Sequence , Chromosome Mapping , Cloning, Molecular , DNA, Complementary , Humans , Hybrid Cells , Introns , Molecular Sequence Data , Polymerase Chain Reaction , Rodentia , Sequence Homology, Nucleic AcidABSTRACT
The 5'-terminal region of the mink S26 ribosomal protein cDNA was cloned using polymerase chain reaction. The nucleotide sequences of the 5'-UTR (24 bp) and a 120-bp fragment of the coding region of RPS26 mRNA were determined. The homology between the coding regions of the human and mink RPS26 mRNAs proved to be 90.8%. The nucleotide sequences of the 5'-UTRs of mink, human, and rat RPS26 mRNAs, as well as mRNAs of the Drosophila S31 protein and Neurospora crp-5 protein, which are homologous to mammalian RPS26, were compared. A highly conserved 9-bp sequence located immediately upstream of the AUG codon was revealed in the 5'-UTR of the RPS26 mRNAs from different species.
