ABSTRACT
Changes in the effect of methotrexate (400 µg/kg nutrient medium) combined with high temperature (28°C) of ontogenesis were studied on two hybrid lines of Drosophila melanogaster heterozygous by the vestigial gene that underwent long-term target selection for cutting injury of the wing. Changes in the wing shape, mortality rate, and morphology of polytene chromosomes were studied.
Subject(s)
Methotrexate/pharmacology , Animals , Biological Ontologies , Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Drosophila melanogaster , Hot Temperature , Polytene Chromosomes/metabolismABSTRACT
To explore the influence of psychiatric distress and substance use on viral load suppression in HIV-infected patients taking ART we analyzed socio-demographic characteristics, CD4+ cells count and viral loads, the Symptom Check List-90 and the Addiction Severity Index of 75 patients who had taken ART for at least 6 month. Using viral load data as the marker of ART success, we divided the sample into two groups. Comparison of the groups showed that education, marriage, and female gender are predictors of optimal viral load suppression. Overall results failed to demonstrate substance use and psychiatric distress as predictors of viral load suppression.
Subject(s)
Anti-HIV Agents/therapeutic use , CD4-Positive T-Lymphocytes/immunology , Depression/diagnosis , HIV Infections/drug therapy , Stress, Psychological , Viral Load/drug effects , Adult , CD4 Lymphocyte Count , Depression/psychology , Female , HIV Infections/psychology , HIV Infections/virology , Humans , Male , Medication Adherence , Middle Aged , Russia/epidemiology , Socioeconomic Factors , Substance-Related Disorders/complications , Substance-Related Disorders/epidemiology , Substance-Related Disorders/psychology , Surveys and QuestionnairesABSTRACT
In the paper there are presented the main results of the performed study on comparative legal analysis of national legislative acts in the field of providing of children's rights for health care in institutions of various types for the delivery of recommendations for harmonization of legislation of States--members of the Eurasian Economic Community.
Subject(s)
Child Welfare/legislation & jurisprudence , Delivery of Health Care/legislation & jurisprudence , Patient Rights/legislation & jurisprudence , Schools , Adolescent , Asia , Child , Europe , Humans , International Cooperation/legislation & jurisprudenceABSTRACT
UNLABELLED: The frequency of micronuclei (MN) in the oral mucosal epitheliocytes, the health status, the environment, and living, upbringing, and education conditions were studied in 5-6-year-old children and 15-16-year-old adolescents living in Saint Petersburg. OBJECTIVE: to identify cytogenetic disorders in the somatic cells of the buccal epithelium in the children and adolescents residing in the areas with different degrees of toxic ambient air and soil pollution. METHODS: hygienic, sociological, mathematical-and-statistical, and cytogenetic (micronucleus test) studies. The method for studying the frequency of micronuclei in the buccal epithelial cells was used for the first time in the population-based studies to evaluate the cytogenetic effect of different levels of chemical ambient air and soil pollution. The genetic disorders have been identified in the somatic cells as the increased count of micronuclei-containing epitheliocytes, which characterize the adverse effect of the high content of toxicants in the air ground layer and soil, as well as a higher sensitivity to unfavorable exogenous factors in 5-6-year-old children, girls in particular.
Subject(s)
Air Pollutants/toxicity , Epithelial Cells/drug effects , Micronuclei, Chromosome-Defective/chemically induced , Mouth Mucosa/drug effects , Soil Pollutants/toxicity , Adolescent , Air Pollutants/analysis , Child , Child, Preschool , Cities , Epithelial Cells/ultrastructure , Female , Humans , Male , Micronucleus Tests , Mouth Mucosa/ultrastructure , Russia , Soil Pollutants/analysisABSTRACT
A novel mode of the tumor suppressor protein p53 regulation, mediated by recruitment of the linker histone H1 to the promoters of p53 target genes leading to specific repression of p53-dependent transcription, has recently been uncovered. Yet, how this repression could be relieved is not clear. Previously, a histone-binding nuclear protein prothymosin alpha (ProTa) was shown to trigger a p53 response. The histone-binding region of ProTa was found to be essential for this effect, raising a possibility that ProTa stimulates p53-dependent transcription by dissociating the p53-histone H1 repressive complex. Here, we have shown that ProTa interacts with the same C-terminal domain of histone H1 as p53 does and, therefore, ProTa and p53 could compete for binding to histone H1. Furthermore, ProTa, when competent for histone H1 binding, is able to liberate p53 from the histone H1-p53 complex in vitro. In vivo, stimulation of p53-dependent transcription by ProTa correlates with ability of ProTa to interact with histone H1. Ectopic expression of histone H1 or its C-terminal ProTa-binding domain specifically suppresses the stimulating effect of ProTa on transcription of the p53-responsive reporter gene in cultured cells. These results are consistent with the model that ProTa may enhance p53 transcription activity by displacement of histone H1 from p53-H1 repressive complex.
Subject(s)
Histones/metabolism , Protein Precursors/metabolism , Thymosin/analogs & derivatives , Transcriptional Activation , Tumor Suppressor Protein p53/metabolism , Genetic Vectors , HeLa Cells , Humans , Promoter Regions, Genetic , Protein Binding , Protein Precursors/genetics , Protein Structure, Tertiary , Thymosin/genetics , Thymosin/metabolism , Transcription, Genetic , Tumor Suppressor Protein p53/geneticsABSTRACT
The authors analyze their experience with diagnosis and treatment of 182 children with syndrome of portal hypertension (PH) from 1991 through 2010. Two groups of patients were considered. The first group included 74 newborns with high risk of the development of PH (infants after catheterization of the umbilical vein who endured omphalitis with USI diagnosed thrombosis of the portal vein, patients with cavernous transformation of the portal vein, hepatosplenomegaly). The second group consisted of 108 children aged from 6 months to 14 years with realized syndrome of PH. Investigation of hemostasis (international normalized ratio, fibrinogen, VIII and IX factors) immunogram of the 2nd level, determination of gene polymorphism (prothrombin, V factor, MTHFR, PAI-1), Bonacini index were included in the complex of examination, besides clinical and biochemical analyses of blood. The degree of disturbance of hemodynamics in the portal system was estimated by the data of USI, dopplerography, FEGS. It was established that children with extrahepatic portal hypertension have markers of hereditary thrombophilia in 98% of cases. Bonacini index allows determination of early signs of the development of secondary fibrosis of the liver in children with PH without using liver biopsy.
Subject(s)
Hypertension, Portal/diagnosis , Hypertension, Portal/therapy , Adolescent , Child , Child, Preschool , Female , Gastrointestinal Hemorrhage/etiology , Gastrointestinal Hemorrhage/prevention & control , Humans , Hypertension, Portal/complications , Infant , Infant, Newborn , Liver Cirrhosis/diagnosis , Liver Cirrhosis/etiology , Liver Cirrhosis/prevention & control , Male , Severity of Illness Index , Splenomegaly/diagnosis , Splenomegaly/etiology , Thrombophilia/complications , Thrombophilia/diagnosisABSTRACT
Influence of ion Mn2+ and Fe2+ on ability of flavonoids intercept free radical and reveal radioprotection activity towards yeast cells was investigated. In work have been used flavonols quercetin, rutin and anthocyanins allocated of flowers Hibiscus. As a measure of radiating damage of cages of yeast the exit of ions K+ served. It is shown that Fe2+ ions essentially reduce protection activity flavonoids, and the ions of manganese showing protection properties, at presence anthocyanins sensibilization radiation damage.
Subject(s)
Antioxidants/pharmacology , Flavonoids/pharmacology , Iron/pharmacology , Manganese/pharmacology , Radiation-Protective Agents/pharmacology , Saccharomyces cerevisiae/drug effects , Anthocyanins/isolation & purification , Anthocyanins/pharmacology , Drug Antagonism , Flowers/chemistry , Free Radicals/antagonists & inhibitors , Hibiscus/chemistry , Potassium/metabolism , Quercetin/pharmacology , Rutin/pharmacology , Saccharomyces cerevisiae/metabolismABSTRACT
The portion of group B streptococcal (GBS) sequence encoding the ABC transport system was cloned and sequenced. The genetic region under study included scaAB gene, which was capable of encoding for putative adherence and aggregation factor, and scaR, which was encoding for putative represser. After the insertional inactivation of scaAB gene on the GBS chromosome streptococci increased the tendency for aggregation. The level of GBS adherence to vaginal epithelium was also different after scaAB inactivation. GBS sea operon was compared to similar ABC encoding bacterial operons.
Subject(s)
Adhesins, Bacterial/genetics , Bacterial Proteins/genetics , Genes, Bacterial , Streptococcus agalactiae/genetics , Streptococcus agalactiae/pathogenicity , Bacterial Adhesion , Cloning, Molecular , Epithelium/microbiology , Female , Humans , Mutagenesis, Insertional , Sequence Analysis, DNA , Vagina/cytology , Virulence/geneticsABSTRACT
The paper deals with problems in teaching adolescents at different levels of education (medical lyceum--higher educational establishment (HEE)). Their health status, functional changes that can vary with the pattern of training are comprehensively assessed. The school-HEE program was found to be effective as the high progress of lyceum students causes worse health. The students' high process and low morbidity within an academic year is suggestive of their rapid adaptation to HEE studies.
Subject(s)
Health Status , Schools , Adolescent , Age Factors , Data Interpretation, Statistical , Humans , RussiaABSTRACT
The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
