[Action of high hydrostatic pressure on the fluorescence of respiratory metabolic components and on the staining capacity of the skeletal muscles in the frog]. / Deistvie vysokogo gidrostaticheskogo davleniia na fluorestsentsiiu komponentov dykhatel'nogo metabolizma i tinktorial'nuiu sposobnost' skeletnykh myshts liagushki.

The effect of high hydrostatic pressure (100-1400 at) on frog skeletal muscle was studied. Superthreshold pressures (above 600 at) accompanied by muscle contracture results in the intensification of sorption of both basic (neutral red) and acid (phenol red) dyes, and in the growth of the number of oxidated forms of respiratory metabolic components--flavoproteins and pyridine nucleotides under study. Subthreshold pressures reduce the tinctorial ability of muscles and decrease oxidative processes caused by the maintenance of muscle in small volume of the Ringer solution.

[Dependence of the rate of development of Zenker's necrosis in the fibers of the frog sartorius muscle on the concentration of chloral hydrate, urea and urethane]. / Zavisimost' skorosti razvitiia tsenkerovskogo nekroza v voloknakh portniazhnoi myshtsy liagushki ot kontsentratsii khloralgidrata, mocheviny i uretana.

The spreading necrosis in dissected m. sartorius of Rana temporaria changes its speed in the phasic mode in relation to the concentrations of chloral hydrate, urea and uretan, added to the Ringer solution. The number and size of the phases are also dependent on the temperature and the initial physiological state. The concentrations of the above chemicals decelerating necrosis are 2-4 times less than those that increase the survival time of isolated muscles.

[Effect of methanol, ethanol, propanol, chloral hydrate, and potassium chloride on the stability of rabbit skeletal muscle actomyosin]. / Vliianie metanola, étanola, propanola, khloralgidrata i khloristogo kaliia na stabil'nost' aktomiozina skeletnykh myshts krolika.

The denaturation time (DT) of rabbit natural actomyosin (AM) and of actomyosin thread models has been detected under conditions of storage in the presence of methanol, ethanol, propanol, chloralhydrate or potassium chloride. DT--is the period of time during which, with the addition of ATP, actomyosin preparations maintain their ability to superprecipitation, and thread models--to contraction. All the organic substances examined taken in definite concentrations, were shown to increase the actomyosin DT as compared with the control probes. The greatest increase of DT was observed with the concentrations of methanol, ethanol and propanol being 8, 4 and 2% (v/v), resp. A dependence of the actomyosin DT on KCl concentration was noted. The thread model DT increased in the presence of ethanol--4% (v/v), and chloralhydrate--0.1 and 0.2% (v/v).

[Dependence of frog epithelial cell and neuron survival time on the concentration of certain substances]. / Zavisimost' vremeni perezhivaniia épitelial'nykh kletok i neironov liagushki ot kontsentratsii nekotorykh veshchestv.

The frog cornea epitelial cells, nervous cells of spinal ganglia and of urinary bladder have been found to survive in vitro, in the presence of subtoxical concentrations of chloral hydrate, urea, ethanol or methanol added to the Ringer solution, longer than control preparations. The above drugs added both in lower and higher concentrations lead to cell damages, thus reducing the cell survival time, which was tested by the capacity to deposit granules of a vital dye - neutral red.

[Increase in stability of isolated tissues and proteins caused by the action of chemical stimulants]. / Uvelichenie ustoichivosti izolirovannykh tkanei i belkov, vyzvannoe deistviem khimicheskikh razdrazhitelei.

Chemical agents in subtoxic concentrations prolongate the survival time of living muscles and the time of contractile ability of glycerinated muscles. They preserve also the native property of actomyosine over the control time. The increase of resistance of living muscles and their models may be explained by stabilization of proteins or of their complexes. It is accompanied by the change of the indices of the functional state of the living muscle--by the decrease of the binding of vital dyes, by the hyperpolarization, by reducing the consumption of O2 and by the conservation of the quantity of SH groups at the high level.

[Increase in the time of conservation of contractility of glycerinated muscles exposed to subthreshhold concentrations of chemical agents]. / Uvelichenie vremeni sokhraneniia sokratimosti glitserinizirovannykh myshts liagushki pod vozdeistviem khimicheskikh agentov podporogovoi kontsentratsii

The response of glycerinized frog muscles to the action of chemical agents at subthreshold concentrations was the same as that of living muscles. Preservation of contractility (up to 50--200% over the control time) in response to ATP was achieved by incubation of the muscle models in solutions of chloral hydrate (0.0038--0.015M), urethan (0.09--0.35M), urea (0.5M) and sodium chloride (0.035--0.070M). Concentrations of these agents were always about 15--45% of the toxicity threshold. The prolongation of the "survival time" is suggested to depend on changes in the contractile proteins of muscles.