ABSTRACT
Cultures of mesenchymal cells from human decidual tooth pulp were derived. The phenotype and capacity to osteogenic and adipogenic differentiation of these cells are close to those of bone marrow mesenchymal stem cells. Decidual tooth pulp mesenchymal cells populate biodegraded polylactide scaffolds and hence, can be used for the creation of tissue engineering transplants for bone defect repair. Storage of decidual tooth pulp mesenchymal cells in the stem cell cryobanks together with umbilical blood will appreciably extent the periods of age for collection of juvenile autologous stem cells for use throughout the life span.
Subject(s)
Bone and Bones , Dental Pulp/cytology , Mesenchymal Stem Cells/cytology , Tissue Engineering/methods , Tooth, Deciduous/cytology , Cell Culture Techniques/methods , Cell Differentiation/physiology , Flow Cytometry , Humans , Immunohistochemistry , Microscopy, Fluorescence , Polyesters , Tissue ScaffoldsABSTRACT
The results of the development and introduction of universal production standards for cell products of mesenchymal origin are presented: technology for obtaining and culturing of primary cell cultures from human postnatal organs and tissues, cell product quality and safety control procedures, methods for cell product storage and transportation, and the necessary files.
Subject(s)
Biological Products/isolation & purification , Biological Products/standards , Biomedical Engineering/standards , Mesenchymal Stem Cells/cytology , Biological Products/biosynthesis , Bone Marrow Cells/cytology , Cell Culture Techniques , Cell Separation/methods , Cell Separation/standards , Cellular Structures , Fetal Blood/cytology , Fibroblasts/cytology , Humans , Infection Control/methods , Quality Control , Skin/cytology , Specimen Handling/methods , Specimen Handling/standards , TransportationABSTRACT
Cell-mediated cytotoxicity was studied in vitro during the interaction of bone marrow mesenchymal stem cells, fibroblast-like cells from newborn umbilical cord, and skin fibroblasts of an adult donor with peripheral blood mononuclear cells. Independently on the origin, mesenchymal cells were not lysed with allogeneic natural killer cells and cytotoxic lymphocytes. Mixed cultures of mesenchymal cells had no cytotoxic effect on peripheral blood mononuclear cells and did not activate proliferation of T and B lymphocytes, natural killer cells, and CD14+ lymphocytes. In vitro experiments showed that mesenchymal cells of different origin and allogeneic immunocompetent cells are tolerant to each other.
