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1.
J Microsc ; 252(3): 286-94, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24118045

ABSTRACT

Recent studies have suggested that silver nanoparticles (AgNPs) may affect cell DNA structure in in vitro conditions. In this paper, we present the results indicating that AgNPs change nuclear complexity properties in isolated human epithelial buccal cells in a time-dependent manner. Epithelial buccal cells were plated in special tissue culture chamber / slides and were kept at 37°C in an RPMI 1640 cell culture medium supplemented with L-glutamine. The cells were treated with colloidal silver nanoparticles suspended in RPMI 1640 medium at the concentration 15 mg L⁻¹. Digital micrographs of the cell nuclei in a sample of 30 cells were created at five different time steps: before the treatment (controls), immediately after the treatment, as well as 15 , 30 and 60 min after the treatment with AgNPs. For each nuclear structure, values of fractal dimension, lacunarity, circularity, as well as parameters of grey level co-occurrence matrix (GLCM) texture, were determined. The results indicate time-dependent reduction of structural complexity in the cell nuclei after the contact with AgNPs. These findings further suggest that AgNPs, at concentrations present in today's over-the-counter drug products, might have significant effects on the cell genetic material.


Subject(s)
Cell Nucleus/drug effects , Epithelial Cells/cytology , Epithelial Cells/drug effects , Nanoparticles/metabolism , Silver/metabolism , Cells, Cultured , Humans , Microscopy , Time-Lapse Imaging
2.
Scand J Med Sci Sports ; 21(2): 260-7, 2011 Apr.
Article in English | MEDLINE | ID: mdl-19895385

ABSTRACT

The aim of this study was to describe qualitatively and quantitatively dietary supplements (DS) and medication use in elite athletes. Athletes (n=912; age 23.9 ± 6 years; 72% male) reported medications and DSs taken within 3 days before doping control. We analyzed data collected from 2006 to 2008, identified and classified substances. Total of 74.6% athletes reported use of at least one substance, 61.2% took DS (3.17 per user) and 40.6% took medications. Among users, 21.2% reported the use of six and more different products, and one took 17 different products at the same time. Majority of medication users took non-steroidal anti-inflammatory drugs (NSAID) (24.7%), and 22.2% used more than one NSAID. We found no gender differences in DS use (P=0.83). Individual sport athletes used more DS (P<0.01). Our study showed widespread use of DS and drugs by elite athletes. Consumption of DS with no evident performance or health benefits, demonstrated the need for specific educational programs focused on DS use. Amount, quantity and combination of the reported products raised concern about the risk of potential side effects.


Subject(s)
Athletes , Dietary Supplements/statistics & numerical data , Nonprescription Drugs , Prescription Drugs , Adolescent , Adult , Anti-Inflammatory Agents, Non-Steroidal , Female , Humans , Male , Middle Aged , Polypharmacy , Young Adult
3.
Physiol Res ; 42(1): 45-7, 1993.
Article in English | MEDLINE | ID: mdl-8329374

ABSTRACT

The effect of chronic hydrocortisone administration (0.5 mg/kg) on the liver and plasma lipid content was assessed in Wistar rats. It was found after that the liver cholesterol content was significantly increased 6 months of hydrocortisone treatment. At the same time, the distribution of liver phospholipid fractions was altered. The fatty acid composition of liver lipids showed a significant increase of 22:6 n-3. Decreased levels of cholesterol and LDL-cholesterol were found in the plasma of the hydrocortisone-treated rats.


Subject(s)
Hydrocortisone/pharmacology , Lipid Metabolism , Liver/drug effects , Administration, Oral , Animals , Cholesterol/blood , Cholesterol/metabolism , Fatty Acids/metabolism , Lipids/blood , Lipoproteins, LDL/blood , Liver/metabolism , Male , Phospholipids/metabolism , Rats , Rats, Wistar
4.
Physiol Bohemoslov ; 39(6): 532-5, 1990.
Article in English | MEDLINE | ID: mdl-2103638

ABSTRACT

The ratio of total concentrations or molar ratios (moles/1,000 amino acid residues) of three non-essential amino acids (glycine, alanine, serine-GAS) and three essential-branched chain amino acids (valine, isoleucine, leucine-BCAA) were investigated in rat systemic and portal vein plasma and jejunal and ileal gut contents after feeding normoprotein (NP) or protein-free (PF) diets for 7 days. Amino acid analysis of gut content showed that the GAS:BCAA ratio was not significantly altered by the PF diet either in the jejunum or in the ileum. On the contrary, the PF diet, caused a three and four-fold increase in this ratio in the portal and systemic plasma, respectively. The situation was produced by the higher concentrations of GAS, which remained near control levels (portal plasma) or exceeded these values (systemic plasma), in contrast to the decreasing levels of BCAA found in both plasmas of the PF group.


Subject(s)
Amino Acids/metabolism , Dietary Proteins , Gastrointestinal Contents , Protein Deficiency/metabolism , Amino Acids/blood , Amino Acids, Essential/metabolism , Animals , Intestine, Small , Portal Vein , Rats , Rats, Inbred Strains , Vena Cava, Inferior
6.
FEBS Lett ; 216(2): 287-90, 1987 Jun 01.
Article in English | MEDLINE | ID: mdl-3582678

ABSTRACT

Amino acids in rat systemic and portal vein plasma and jejunal and ileal gut contents after 7 days of feeding normoprotein (NP) and protein-free diet (PF) are investigated. Amino acid analyses revealed that ingestion of PF diet resulted in unusual amino acid patterns in both plasmas. Thus, while the levels and/or molar ratios of all indispensable amino acids were significantly decreased, those of several gluconeogenetic amino acids, especially of glycine and alanine, were increased in both plasmas, but particularly in portal. By contrast, the molar ratios of the majority of amino acids in jejunal and ileal contents were not changed by PF diet.


Subject(s)
Amino Acids/metabolism , Intestinal Mucosa/metabolism , Amino Acids/blood , Animals , Diet , Female , Homeostasis , Ileum/metabolism , Jejunum/metabolism , Male , Portal System , Rats
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