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Chem Asian J ; 1(4): 575-80, 2006 Oct 20.
Article in English | MEDLINE | ID: mdl-17441095

ABSTRACT

Branched tris-DNA, in which two oligonucleotides of the same sequence and one other oligonucleotide of a different sequence are connected with a rigid central linker, was prepared chemically by using a DNA synthesizer. Two branched tris-DNA molecules with complementary DNA sequences form dimer and tetramer as well as linear and spherical oligomer complexes. The complex formation was studied by UV/thermal denaturation, enzyme digestion, gel electrophoresis, and AFM imaging.


Subject(s)
DNA/chemistry , Nanostructures/chemistry , Tromethamine/chemistry , Base Pairing , Dimerization , Electrophoresis, Agar Gel , Hydrogen-Ion Concentration , Magnetic Resonance Spectroscopy , Microscopy, Atomic Force , Models, Chemical , Nucleic Acid Conformation , Oligonucleotides/chemistry , RNA/chemistry , Temperature , Ultraviolet Rays
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