ABSTRACT
AIM: It is hoped that nanoparticles will become ever more useful in the development of nanomedicine. To evaluate the behavior of nanoparticles in solution, we aimed to establish a single optical fiber-illumination method that is easy to integrate with a conventional microscope at low cost. METHODS: Solutions of gold nanoparticles and carbon nanotubes were analyzed in a single optical fiber-illuminated video microscope and the tracks of Brownian motion of these nanoparticles were traced using video images. Their diffusion coefficient was measured by the mean square displacement of the movement. Using the diffusion coefficient in the Stokes-Einstein equation, the hydrodynamic diameter of the nanoparticles in solution was evaluated. RESULTS: The visualization of gold nanoparticles clearly in a high signal-to-noise ratio was achieved. The evaluated particle sizes of gold nanoparticles were similar to those obtained by a transmission electron microscope and the aggregation process of the carbon nanotubes following incubation was also observed and similar size estimation of the aggregates was performed. CONCLUSION: The single fiber-illumination method was applicable to visualize nanoparticle movement clearly and to estimate their sizes in solution. This simple method is suitable for the in situ observation of the nanoparticle-binding process to target cells.
Subject(s)
Colloids/chemistry , Fiber Optic Technology/instrumentation , Image Interpretation, Computer-Assisted/methods , Microscopy, Video/instrumentation , Microscopy, Video/methods , Nanoparticles/ultrastructure , Pattern Recognition, Automated/methods , Algorithms , Artificial Intelligence , Colloids/analysis , Equipment Design , Equipment Failure Analysis , Image Interpretation, Computer-Assisted/instrumentation , Optical Fibers , Particle Size , Reproducibility of Results , Sensitivity and Specificity , Signal Processing, Computer-Assisted/instrumentation , SolutionsABSTRACT
This study examined whether a hypothermic environment reduces experimentally-induced atrophy of skeletal muscle, as judged by histochemical findings. The hind limbs of hamsters in a hypothermic group were suspended and flexed into plantar positions at the ankle joint, and housed for one week at 8 to 12 degree celsius in a temperature-controlled room, while the normothermic group was housed at 23 to 25 degree celsius. Hypothermia did not significantly alter the average caloric intake, and the animals from the hypothermic group lost a significant amount of body weight when compared with the normothermic group. The hypothermic group retained more muscle wet-weight and myofibers cross-sectional area in the soleus and gastrocnemius muscles compared with the normothermic group. Our results indicate that a hypothermic environment inhibits short-term muscle atrophy. This inhibition may be caused by the increased caloric intake combined with a state similar to hibernation in low-temperature environments.
Subject(s)
Muscular Disorders, Atrophic/physiopathology , Temperature , Animals , Cricetinae , Extremities , Hindlimb Suspension/physiology , Hypothermia, Induced , MesocricetusABSTRACT
The nano-kinetic movement of a single DNA molecule was observed and analyzed by a newly developed video-microscope system with an optical fiber, called a pin-fiber video scope. A single lambda-DNA molecule was put in focus using fiber-illumination, and the stretching and shrinking motion was measured. The molecule's kinetics were analyzed by numerical calculations and are discussed. A photocleavage phenomenon of DNA molecules was also visualized by the pin-fiber video scope. The new video-microscope system has the potential to observe and analyze the nano-kinetics of a single molecule.
Subject(s)
DNA/metabolism , Nanotechnology/methods , Fiber Optic Technology , Humans , Microscopy, Video , Optical FibersABSTRACT
The nano-kinetics of colloidal particles and living cells with the colloidal particles were visualized by a newly developed video scope. The system of the new video scope has a feature of fine controlling the illumination conditions by using a single optical fiber. This characteristic enables one to obtain clear images of living cells and the motions of colloidal particles by light-scattering effects. In the experiments, RBL-2H3 cells and gold colloidal particles were observed. Scattering images with high contrast and a dark background like in dark-field observations could be attained. In the experiments, a pulsed laser was also applied. The results obtained in this study could validate the effectiveness and possibility of a new video scope for applications to biological and biomedical fields.
