ABSTRACT
NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.
Subject(s)
Cryopreservation , NADPH Oxidases/metabolism , Neutrophils/cytology , Pectins/metabolism , Phagocytosis , Cells, Cultured , Cryopreservation/methods , Enzyme Activation , Humans , Neutrophils/metabolism , Reactive Oxygen Species/metabolismABSTRACT
It was shown that the use of little toxic cold-protective containing the glycerol protector endocellular action, HES--protector exocellular action, and "restored" component of the succinate 3-hydroxy-6-methyl-2- ethylpyridine preserves nuclear blood cells in a viable condition at -40 degrees C.
Subject(s)
Blood Preservation/methods , Cryopreservation/methods , Cryoprotective Agents/pharmacology , Glycerol/pharmacology , Leukocytes/cytology , Picolines/pharmacology , Succinates/pharmacology , Female , Freezing , Humans , MaleABSTRACT
The intensive metabolism and fast exhaustion of an energy potential of nuclear blood cells at positive temperatures essentially reduce terms of their storage in a viable condition. However for the clinical purposes given thensfusion environment is the extremely necessary. The popular method of preservation of cells at ultralow temperature (-196 degrees C) is limited in application since assumes use of the liquid nitrogen, the special equipment, trained engineering personnel. The work is devoted to development effective and simple in execution of a method of preservation of leukocytes in conditions of temperatures about zero (+2 degrees--0 degrees C) with use of a household elecrorefrigerator and new cryopreservation a solution.
Subject(s)
Blood Preservation/methods , Leukocytes/cytology , Adult , Cell Survival , Cold Temperature , Female , Humans , Male , Middle AgedABSTRACT
Choline chloride in complex with the main cryophylactic (1,2-propanediol) preserves morphological integrity and functional activity of blood nuclears after freezing to -40 degrees C by the exponential program.
Subject(s)
Choline/pharmacology , Cryopreservation/methods , Cryoprotective Agents/pharmacology , Leukocytes , Humans , Leukocytes/cytology , Leukocytes/drug effects , Leukocytes/metabolism , Propylene GlycolSubject(s)
Araceae/chemistry , Cryoprotective Agents/isolation & purification , Pectins/isolation & purification , Cell Count , Cell Survival/drug effects , Cryopreservation/methods , Cryoprotective Agents/pharmacology , Cytapheresis , Humans , Leukocytes/cytology , Leukocytes/drug effects , Neutrophils/cytology , Neutrophils/drug effects , Pectins/pharmacology , Phagocytosis/drug effects , Time FactorsABSTRACT
AIM: To assess efficacy of vaccine against viral hepatitis B (VHB) in adults after achievement of acute leukemia (AL) remission. MATERIAL AND METHODS: Specific prevention of VHB was made in 30 AL patients with recombinant vaccine Engerix B by two schemes: 0-1-2-12 and 0-1-2-6 months with double adult dose 40 mcg. Efficacy of vaccination was evaluated by the titer of antibodies to HBs-Ag (HBs-Ab) 1 month after each injection of the vaccine. The control group consisted of 36 patients. Follow-up was 6 months to 10 years. RESULTS: Vaccine prophylaxis of VHB in AL remission resulted in appearance of the protective antibody titer after the forth vaccine injection in 50% patients. Out of 30 vaccinated patients 2 (6.7%) got infected with HBV 3 and 5 years after onset of the disease. Markers of HBV replication were detected in 8 (22%) of 36 patients. CONCLUSION: Vaccine prophylaxis of VHB in adult patients in AL remission was effective in 93.3% of the vaccinated patients.
Subject(s)
Hepatitis B Vaccines/therapeutic use , Hepatitis B/prevention & control , Leukemia, Myeloid, Acute/complications , Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications , Vaccination/methods , Adult , Female , Follow-Up Studies , Hepatitis B/complications , Hepatitis B Antibodies/immunology , Hepatitis B Surface Antigens/immunology , Hepatitis B e Antigens/immunology , Hepatitis B virus/immunology , Humans , Male , Treatment OutcomeABSTRACT
We propose a new effective, available, and economic method for preservation of leukocytes at near-zero temperatures for 6 days using an original protecting solution.
Subject(s)
Cell Survival , Cryopreservation/methods , Leukocytes , Humans , Leukocytes/cytology , Leukocytes/metabolismABSTRACT
A new effective and available method is proposed for preservation of human blood leukocytes at supercooling temperature (-10 degrees C). The method consists in induction of cold hypobiosis under protection of an original antifreeze protecting solution containing glycerol (cryoprotector), mexidol (antioxidant and membrane stabilizer), and gelatin preparation (modegel or gelatinol) as the solvent. No washing of the solution from the biological object before its intravenous injection is needed; the solution preserves morphological integrity of cells and their functional activity at a high level for 12 days.
Subject(s)
Blood Preservation/methods , Gelatin/chemistry , Leukocytes/cytology , Solutions/chemistry , Cryoprotective Agents/chemistry , Humans , Leukocytes/metabolism , Reproducibility of Results , Time FactorsABSTRACT
We studied the resistance of human blood T and B lymphocytes to cold anabiosis (-20 degrees C, -40 degrees C, and -80 degrees C) of different duration.
Subject(s)
B-Lymphocytes/cytology , Cell Survival/physiology , Cold Temperature , Cryopreservation/methods , T-Lymphocytes/cytology , Cryoprotective Agents , HumansABSTRACT
Investigation of effective and accessible method for administration of human blood leukocytes in the cold anabiosis condition at am oderately low temperature of minus 20 degrees C by the exponential program with cryopreservative which does not require washing from the bio-object after its thawing, has been proposed. Morphological and functional properties of the leukocytes after different periods of conservation in the state of cold anabiosis were studied. The data obtained suggest that optimal conservation of the morpho-functional properties of cells with the aid of this method equals 3 weeks.
Subject(s)
Blood Preservation , Cryopreservation , Leukocytes , Blood Preservation/methods , Cold Temperature , Cryopreservation/methods , Humans , Leukocytes/cytology , Leukocytes/physiologyABSTRACT
Morphological and functional indicators were investigated of donor leukocyte concentrates mixed at a ratio of 1 to 1 with three versions of cold-protective and kept at an undercooling temperature (-10 degree C) for 24 hours, and with a cryoprotector of optimal composition for 12 days,. An optimal version of a non-freezing cold-protective solution was found. It was determined that 76+/-4 % of the neutrophils retain there ability of phagocytosis among the viable cells (81+/-5 %) after a 12 day hypobiosis. Thus the results testify to a high morphofunctional safety of human blood leukocytes which experienced cold-hypobiosis (-10 degree C) in an original non-freezing solution not requiring washing after re-warming.
Subject(s)
Cryopreservation , Leukocytes/cytology , Cryoprotective Agents , Eosine Yellowish-(YS) , Fluorescent Dyes , Humans , Leukocytes/physiology , Neutrophils/physiology , PhagocytosisABSTRACT
We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40 degrees C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.
Subject(s)
Blood Preservation , Cryopreservation , Lymphocytes/physiology , Neutrophils/physiology , Phagocytosis , Cryoprotective Agents , Humans , In Vitro Techniques , Time FactorsABSTRACT
We propose a simple, effective, and economic method for introducing human blood leukocytes in cold anabiosis at moderately low temperature (-50 degrees Celsius).