Protein and Long-Chain n-3 Polyunsaturated Fatty Acids Recovered from Herring Brines upon Flocculation and Flotation-A Case Study.

A novel integrated process for recovery of protein-enriched biomasses from 5% presalting brines and spice brines of herring (Clupea harengus) was investigated by combining carrageenan- and/or acid-driven flocculation (F) plus dissolved air flotation (DAF). The F-DAF technique with carrageenan resulted in protein and lipid recoveries from 5% presalting brine of 78 and 38%, respectively. Without flocculation or with only acidification, protein and lipid recoveries in DAF were only 13 and 10%, respectively. Low protein and lipid recoveries, 8-12 and 1.8-8.2%, respectively, were also obtained when spice brine was subjected to only acidification and DAF. The protein content in dry biomasses from 5% presalting brine and spice brine was 36-43 and 13-16%, respectively. The corresponding lipid levels were 23-31 and 9-18%, respectively, with ash levels of 11-20 and 38-45%, respectively. Biomass proteins contained ≤45% essential amino acids, and the lipids had ≤16% long-chain n-3 polyunsaturated fatty acids. Freeze-dried spice brine biomasses were characterized by anchovy- and spice-related sensory attributes. 5% presalting brine biomasses were connected to fish and seafood attributes and showed gel forming capacity. The outlined F-DAF recovery system can thus recover both nutrients and interesting flavors from the herring process waters, which are currently lost from the food chain.

Changes in patterns of persistent halogenated compounds through a pelagic food web in the Baltic Sea.

The concentrations and patterns of persistent halogenated compounds (PHCs), including polychlorinated biphenyls (PCBs), DDT, hexachlorocyclohexanes (HCHs), hexachlorobenzene (HCB) and polybrominated diphenyl ethers (PBDEs) were examined in a pelagic food web from the southern Baltic Sea consisting of sediment, zooplankton, sprat, Atlantic salmon and anadromous brown trout. Lipid-normalized concentrations generally increased from low trophic levels to high trophic levels, with the exception of HCHs. Due to high concentrations of PBDEs in some zooplankton samples, biomagnification of BDE-47 was only observed for salmon/sprat and trout/sprat. Sprat collected individually and from salmon stomach had significantly different lipid-normalized concentrations and varied in their PHC pattern as well, possibly indicating a large natural variation within the Baltic Sea. The highest lipid-normalized concentrations were found in brown trout. Salmon and brown trout were similar in their PHC pattern suggesting similar food sources. Variation in PHC patterns among trophic levels was not smaller than that among geographically distinct locations, confirming the importance of comparable trophic levels for the assessment of PHC patterns, e.g. for tracing migratory fish.

Polychlorinated biphenyls, organochlorine pesticides and polycyclic aromatic hydrocarbons in a one-off global survey of bivalves.

During the Danish Galathea 3 expedition, bivalve samples were collected at the Faroe Islands, Greenland, Ghana, South Africa, Australia, Solomon Islands, New Zealand, Chile, US Virgin Islands, Boston, Newfoundland and Shetland Islands and analysed for organochlorines and PAHs. Concentration differences of up to three orders of magnitude were observed, with the highest concentrations at Boston harbour (SPCB 338 ng g(-1) dw, ΣSPAH 5966 ng g(-1) dw) and the Sydney estuary (ΣSPCB 282 ng g(-1) dw, SPAH 1453 ng g(-1) dw). Local impacts were also found for the Greenland capital Nuuk in terms of PCB and PAH levels, while other Greenland samples came closest to representing PAH background levels. Several locations had undetectable organochlorine levels, including Hobart and Chile, which had the lowest SPAH concentrations (<200 ng g(-1) dw). It was possible to group the stations according to their pyrogenic/petrogenic influence using Principal Component Analyses, and indications of petroleum sources were found at Nuuk.

Adhesion characteristics of nitrifying bacteria in activated sludge.

Nitrifiers are known to form relatively dense and strong microcolonies in activated sludge flocs, but little is known about their adhesion characteristics and how these are relative to other floc components. The size distribution of ammonia and nitrite-oxidizing bacteria (Nitrosomonas oligotropha and Nitrospira spp.) in activated sludge from a nutrient removal plant showed that the majority of N. oligotropha cells formed microcolonies with a diameter of 13-22.5 microm, and most Nitrospira spp. cells formed microcolonies with a diameter of 9-22.5 microm. By applying high shear forces (2200 s(-1)), the largest microcolonies of N. oligotropha fragmented to a level well below the Kolmogorov microscale (approx. 15-25 microm). Only very little erosion of single cells took place. Nitrospira spp. microcolonies were generally slightly stronger than N. oligotropha. In order to characterize the adhesion/binding mechanisms for the individual microcolonies, a number of different physico-chemical treatments were combined with shear, and even though this did not lead to any explicit characterization of the species-specific adhesion mechanisms, entanglement of extracellular polymers was proposed as a plausible important adhesion mechanism. When compared to other floc components, the deflocculated fractions of N. oligotropha and Nitrospira spp. were much lower than those of cells in general (total cell count, DAPI) or the organic matter. Deflocculation of N. oligotropha ranged from 3% to 11% of the total N. oligotropha biovolume, Nitrospira spp. from 1% to 4% of the total Nitrospira spp. biovolume, whereas the number of deflocculated cells was 9-54% of the total cell count, and the deflocculated organic matter constituted 8-43% of the total amount of organic matter. These results clearly showed that activated sludge contained a large pool of loosely attached cells and extracellular polymeric substances, and that the nitrifiers and some other microcolony formers were very strong and remained almost intact even under extreme physical and chemical conditions.

Body burdens of persistent halogenated compounds during different development stages of anadromous brown trout (Salmo trutta).

Polychlorinated biphenyls (PCBs), DDTs, and polybrominated diphenyl ethers (PBDEs) were followed through the five life stages of a wild population of anadromous brown trout and related to variations in lipid content and exposure situations. Anadromous brown trout exhibits great variations in lipid content during its life cycle in the freshwater and marine environments. The results indicated substantial differences in PBDE and organochlorine exposure, with apparently more recent sources of PBDEs in the freshwater environment relative to the marine environment. Lipid and contaminant transfer were not always identical: The concentrations of PCBs, DDTs, and PBDEs (ng/g lipid weight) were about 15 times lower in the eggs compared to the muscle of their mother (e.g., 823 ng PCB/g Iw vs. 12,565 ng PCB/g lw, respectively). During the starving period from maiden to spawning trout the contaminant load increased by a higher factor than the lipid use. The data suggest a decoupling between lipid content and organohalogen concentrations for anadromous brown trout, which may contribute positively to reduce any potential negative effects of the transferred contaminants on eggs and fry.