Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Am J Pathol ; 163(5): 1921-8, 2003 Nov.
Article in English | MEDLINE | ID: mdl-14578191

ABSTRACT

Elevated semicarbazide-sensitive amine oxidase (SSAO) activity has been observed in several human conditions, eg, diabetes, and it has been speculated that SSAO contributes to the development of vasculopathies associated with this disease. To investigate in vivo consequences of elevated expression of SSAO in vascular tissues, we have developed a transgenic model for overexpression of human SSAO in mice. A smooth muscle-specific promoter, smooth muscle alpha-actin promoter 8 (SMP8) was used. Transgenic expression of human SSAO in tissues with a high content of smooth muscle cells was confirmed by Northern blot analysis. Enzymatic analysis of homogenates from transgenic tissues showed elevated levels of SSAO activity compared to non-transgenic littermates. Furthermore, when plasma SSAO activity was analyzed, much higher activity was detected compared to plasma from control mice, indicating that plasma SSAO may originate from smooth muscle cells. Histopathological evaluation of aorta and renal artery from transgenic mice revealed an abnormal structure of the elastin tissue. Instead of the regularly folded elastic laminae normally found in tunica media of sacrificed mice, the elastic laminae were straight and unfolded with irregularly arranged elastic fibers, forming tangled webs, between the intercalating elastic laminae. These alterations of the elastin structures suggest that overexpression of SSAO has led to a reduced elasticity of the arteries. Moreover, the mean femoral arterial pressure of the SMP8 SSAO transgenic mice was significantly lower in comparison to non-transgenic littermates. This suggests that the transgenic mice have a defect in their ability to regulate blood pressure.


Subject(s)
Amine Oxidase (Copper-Containing)/biosynthesis , Aorta/pathology , Myocytes, Smooth Muscle/enzymology , Amine Oxidase (Copper-Containing)/blood , Amine Oxidase (Copper-Containing)/genetics , Animals , Aorta/enzymology , Aorta/ultrastructure , Arteries/pathology , Arteries/physiopathology , Blood Pressure/genetics , Blood Pressure/physiology , Blotting, Northern , Elastin/ultrastructure , Humans , Mice , Mice, Transgenic , Promoter Regions, Genetic , Up-Regulation
2.
Glia ; 41(3): 276-89, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12528182

ABSTRACT

Platelet-derived growth factor (PDGF) influences the generation of neurons and glia during embryogenesis and in early postnatal life. In an attempt to determine the consequences of an overexpression of PDGF-B during the first weeks of life, we targeted transgenic expression of a human PDGF-B cDNA to myelinating tracts using the promoter region of the myelin basic protein (MBP) gene. Transgenic mRNA and protein were expressed in the brain and the expression profile of the human PDGF-B during early postnatal development closely paralleled that of the endogenous mouse MBP gene. The gross morphological appearance of transgenic brains was normal but at the cellular level several phenotypic alterations could be identified. In white matter tracts such as the corpus callosum and cerebellar medulla, there was a marked hypercellularity. The number of oligodendrocyte precursors was increased and astrocytes were more abundant. In adult mice carrying the MBP-PDGF-B transgene, however, myelination appeared normal and the amount of oligodendrocytes was similar to that of control littermates. In addition to the phenotypic alterations in the brain, investigation of eye structure revealed a striking disorganization of retinal architecture. The retina was folded with cells collected in papillar or follicular-like structures. Retinal whole mount preparations after India ink perfusion revealed capillary disorganization with large-caliber vessels supporting only a few fine branches. Our observations strengthen the notion that PDGF is an important effector molecule in postnatal CNS development.


Subject(s)
Oligodendroglia/pathology , Proto-Oncogene Proteins c-sis/genetics , Retina/abnormalities , Stem Cells/pathology , Animals , Astrocytes/pathology , Capillaries/abnormalities , Capillaries/pathology , Cell Count , Female , Gene Expression Regulation, Developmental , Male , Mice , Mice, Inbred C3H , Mice, Transgenic , Microglia/pathology , Nerve Fibers, Myelinated/pathology , Nerve Fibers, Myelinated/physiology , Pregnancy , RNA, Messenger/analysis , Retina/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...