ABSTRACT
A new method of labelling short ragweed pollen antigens with carbon-14 is described. The known ragweed antigens AgE, Ra3, Ra5 and several other antigens are radiolabelled. These components are biologically active, have a high specific radioactivity and a long half-life.
Subject(s)
Antigens , Carbon Radioisotopes , Isotope Labeling/methods , Pollen , Trypanosoma cruzi , Antigens/analysis , Antigens/isolation & purification , Histamine Release , Plant Extracts/immunology , Plant Extracts/standards , Pollen/immunologySubject(s)
HeLa Cells/metabolism , Hot Temperature , Cell Line , Cell Nucleolus , Cell Nucleus/analysis , Chromosomes/analysis , Cytoplasm/drug effects , Dactinomycin/pharmacology , Endoplasmic Reticulum/analysis , Fibroblasts/cytology , Golgi Apparatus/analysis , Histocytochemistry , Humans , Lysosomes/analysis , Methods , Microtubules , Mitochondria/analysis , Nucleic Acids/analysis , Pepsin A/pharmacology , RNA, Neoplasm/biosynthesis , Ribonucleases/pharmacology , Ribosomes/analysis , Ribosomes/drug effects , Time Factors , Tritium , UridineSubject(s)
Antibiotics, Antineoplastic/pharmacology , Avian Leukosis/metabolism , RNA, Viral/biosynthesis , Animals , Antibiotics, Antineoplastic/therapeutic use , Avian Leukosis/pathology , Avian Leukosis Virus , Centrifugation, Density Gradient , Chick Embryo , Fibroblasts/metabolism , In Vitro Techniques , RNA, Neoplasm/biosynthesis , Ribosomes/metabolismABSTRACT
Strain MC29 avian leukosis (myelocytomatosis) virus induced infection, elaboration of virus, and morphological alteration in chick embryo cells in vitro. Virus liberation began within 18 hr, morphological change was detectable at about 40 hr, and the cultures could be completely altered within 80 hr after infection. Altered cells were about half the volume and grew at approximately twice the rate of uninfected elements. The output of virus estimated by electron microscopy was about 140 particles per cell per hr. Deoxyribonucleic acid remained constant, but ribonucleic acid increased in both infected and control cells in adjustment to culture environment. The rates of uptake and incorporation of (3)H-uridine and the incorporation of (3)H-thymidine increased in the infected cells with onset of morphological change but were unaffected by processes of infection and virus elaboration per se. Incorporation of a (14)C-amino acid mixture was slightly greater in the infected than in control cells. The speed of continuity of infection and massive morphological alteration constitute a unique response to avian tumor viruses, and the system gives promise of singular value for detailed studies of the processes of infection and morphological change.
