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1.
Synapse ; 42(3): 185-92, 2001 Dec 01.
Article in English | MEDLINE | ID: mdl-11746715

ABSTRACT

The behavioral effects of psychostimulants, which are produced at least in part through inhibition of the dopamine transporter (DAT), are modulated by kappa-opioid receptors (KOR) in the nucleus accumbens (Acb). Using electron microscopic immunocytochemistry, we reveal that in the Acb KOR labeling is mainly, and DAT immunoreactivity is exclusively, presynaptic. From 400 KOR-labeled presynaptic structures, including axon terminals, intervaricosities, and small axons, 51% expressed DAT and 29% contacted another population of terminals exclusively labeled for DAT. Within axonal profiles that contained both antigens, DAT and KOR were prominently localized to plasma membrane segments that showed overlapping distributions of the respective immunogold-silver and immunoperoxidase markers. KOR labeling was also localized to membranes of small synaptic vesicles in terminals with or without DAT immunoreactivity. In addition, from 24 KOR-immunoreactive dendritic spines 42% received convergent input from DAT-containing varicosities and unlabeled terminals forming asymmetric, excitatory-type synapses. Our results provide the first ultrastructural evidence that in the Acb, KOR is localized to strategic sites for involvement in the direct presynaptic release and/or reuptake of dopamine. These data also suggest a role for KOR in the presynaptic modulation of other neurotransmitters and in the postsynaptic excitatory responses of single spiny neurons in the Acb. Dual actions on dopamine terminals and their targets in the Acb may account for KOR-mediated attenuation of drug reinforcement and sensitization.


Subject(s)
Axons/chemistry , Membrane Glycoproteins , Membrane Transport Proteins/analysis , Nerve Tissue Proteins , Nucleus Accumbens/chemistry , Receptors, Opioid, kappa/analysis , Animals , Axons/ultrastructure , Cell Membrane/chemistry , Cell Membrane/ultrastructure , Dendrites/chemistry , Dendrites/ultrastructure , Dopamine/physiology , Dopamine Plasma Membrane Transport Proteins , Immunohistochemistry , Membrane Transport Proteins/biosynthesis , Membrane Transport Proteins/immunology , Microscopy, Immunoelectron , Nucleus Accumbens/cytology , Rats , Receptors, Opioid, kappa/biosynthesis , Receptors, Opioid, kappa/immunology
2.
Synapse ; 41(3): 221-9, 2001 Sep 01.
Article in English | MEDLINE | ID: mdl-11418935

ABSTRACT

Mesocorticolimbic projections originating from dopaminergic and GABAergic neurons in the ventral tegmental area (VTA) play a critical role in opiate addiction. Activation of mu-opioid receptors (MOR), which are located mainly within inhibitory neurons in the VTA, results in enhanced dopaminergic transmission in target regions, including the medial prefrontal cortex (mPFC). We combined retrograde tract-tracing and electron microscopic immunocytochemistry to determine if neurons in the VTA that project to the mPFC contain MOR or receive input from MOR-containing terminals. Rats received unilateral injections of the retrograde tracer Fluoro-Gold (FG) into the mPFC. Tissue sections throughout the VTA were then processed for electron microscopic examination of FG and MOR. Immunoperoxidase labeling for FG was present in VTA cell bodies that contained immunogold-silver particles for MOR that often were contacted by profiles exclusively immunoreactive for MOR, including somata and axon terminals. The majority of dually labeled profiles were dendrites that received convergent input from unlabeled axon terminals forming either symmetric or asymmetric type synapses. Within retrogradely labeled cell bodies and proximal dendrites, MOR immunoreactivity was mainly sequestered within the cytoplasm. In contrast, distal retrogradely labeled dendrites contained MOR gold particles located along the plasma membranes. These data suggest that opiates active at MOR in the VTA modulate cortical activity through 1) presynaptic actions on MOR in terminals contacting mesocortical cell bodies, and 2) direct activation of MOR in distal dendrites of projection neurons.


Subject(s)
Neurons/metabolism , Prefrontal Cortex/cytology , Receptors, Opioid, mu/metabolism , Stilbamidines , Ventral Tegmental Area/cytology , Ventral Tegmental Area/metabolism , Animals , Antibodies , Cell Membrane/chemistry , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Dendrites/chemistry , Dendrites/metabolism , Dendrites/ultrastructure , Dopamine/metabolism , Fluorescent Dyes , Immunoenzyme Techniques , Male , Microscopy, Electron , Neural Pathways , Neurons/chemistry , Neurons/ultrastructure , Presynaptic Terminals/chemistry , Presynaptic Terminals/metabolism , Presynaptic Terminals/ultrastructure , Rats , Rats, Sprague-Dawley , Receptors, Opioid, mu/analysis , Ventral Tegmental Area/chemistry , gamma-Aminobutyric Acid/metabolism
3.
Synapse ; 40(3): 184-92, 2001 Jun 01.
Article in English | MEDLINE | ID: mdl-11304756

ABSTRACT

Cholinergic interneurons in the nucleus accumbens shell (AcbSh) are implicated in the reinforcing behaviors that develop in response to opiates active at mu-opioid receptors (MOR). We examined the electron microscopic immunocytochemical localization of the vesicular acetylcholine transporter (VAChT) and MOR to determine the functional sites for storage and release of acetylcholine (ACh), and potential interactions involving MOR in this region of rat brain. VAChT was primarily localized to membranes of small synaptic vesicles in axon terminals. Less than 10% of the VAChT-labeled terminals were MOR-immunoreactive. In contrast, 35% of the cholinergic terminals formed symmetric or punctate synapses with dendrites showing an extrasynaptic plasmalemmal distribution of MOR. Membranes of tubulovesicles in other selective dendrites were also VAChT-labeled, and almost half of these dendrites displayed plasmalemmal MOR immunoreactivity. The VAChT-labeled dendritic tubulovesicles often apposed unlabeled axon terminals that formed symmetric synapses. Our results indicate that in the AcbSh MOR agonists can modulate the release of ACh from vesicular storage sites in axon terminals as well as in dendrites where the released ACh may serve an autoregulatory function involving inhibitory afferents. These results also suggest, however, that many of the dendrites of spiny projection neurons in the AcbSh are dually influenced by ACh and opiates active at MOR, thus providing a cellular substrate for ACh in the reinforcement of opiates.


Subject(s)
Acetylcholine/metabolism , Membrane Transport Proteins/metabolism , Nucleus Accumbens/metabolism , Presynaptic Terminals/metabolism , Receptors, Opioid, mu/metabolism , Animals , Dendrites/metabolism , Dendrites/ultrastructure , Immunohistochemistry , Interneurons/metabolism , Interneurons/ultrastructure , Male , Microscopy, Electron, Transmission , Narcotics/pharmacology , Neural Inhibition/physiology , Nucleus Accumbens/ultrastructure , Presynaptic Terminals/ultrastructure , Rats , Rats, Sprague-Dawley , Synaptic Membranes/metabolism , Synaptic Membranes/ultrastructure , Synaptic Transmission/physiology , Synaptic Vesicles/metabolism , Synaptic Vesicles/ultrastructure , Vesicular Acetylcholine Transport Proteins
4.
Synapse ; 36(3): 222-32, 2000 Jun 01.
Article in English | MEDLINE | ID: mdl-10819901

ABSTRACT

Dopamine D(4) receptors in the nucleus accumbens shell (AcbSh) are thought to play a key role in mediating the locomotor and sensitizing affects of psychostimulants, as well as the therapeutic efficacy of atypical antipsychotic drugs. We used electron microscopic immunocytochemistry to determine the functional sites for endogenous and exogenous D(4) receptor activation in this region. Of 1,090 D(4) receptor-labeled profiles observed in the AcbSh of rat brain, 65% were axons and axon terminals, while 22% were dendrites and dendritic spines. Within axons and terminals, D(4) receptor immunoreactivity was localized to segments of the plasma membrane and membranes of nearby vesicles. The axon terminals were morphologically heterogenous, varying in size and content of either all small synaptic vesicles (ssv), or ssv and large dense-core vesicles. The labeled terminals occasionally formed asymmetric excitatory-type axospinous synapses, but the majority were without recognizable synaptic specializations. In a separate series of tissue sections that were processed for dual-labeling of the D(4) receptor and the catecholamine synthesizing enzyme, tyrosine hydroxylase (TH), 56% of all observed associations were appositions between differentially labeled axonal profiles, and 17% were terminals that contained immunoreactivity for both antigens. Dendritic spines containing D(4) receptor-labeling also received convergent input from TH-immunoreactive terminals and unlabeled terminals forming asymmetric synapses. These results provide the first ultrastructural evidence for a major presynaptic, and a more minor postsynaptic, involvement of D(4) receptors in dopaminergic modulation of excitatory transmission in the AcbSh.


Subject(s)
Nucleus Accumbens/metabolism , Presynaptic Terminals/metabolism , Receptors, Dopamine D2/metabolism , Animals , Axons/metabolism , Axons/ultrastructure , Dendrites/metabolism , Dendrites/ultrastructure , Immunohistochemistry , Male , Microscopy, Electron , Nerve Endings/metabolism , Nerve Endings/ultrastructure , Nucleus Accumbens/ultrastructure , Rats , Rats, Sprague-Dawley , Receptors, Dopamine D4 , Tissue Distribution , Tyrosine 3-Monooxygenase/metabolism
5.
Synapse ; 34(1): 1-10, 1999 Oct.
Article in English | MEDLINE | ID: mdl-10459166

ABSTRACT

Opiate- and psychostimulant-induced modulation of dopamine transmission in the nucleus accumbens shell (AcbSh) is thought to play a key role in their potent reinforcing and locomotor effects. To investigate the cellular basis for potential functional interactions involving opiates active at the delta-opioid receptor (DOR) and psychostimulants that bind selectively to the dopamine transporter (DAT), we examined the electron microscopic localization of their respective antisera in rat AcbSh. DOR immunoperoxidase labeling was seen primarily, and DAT immunogold particles exclusively, in axon terminals. In these terminals, DOR immunoreactivity was prominently associated with discrete segments of the plasma membrane and the membranes of nearby small synaptic and large dense core vesicles. DAT immunogold particles were almost exclusively distributed along nonsynaptic axonal plasma membranes. Thirty-nine percent DOR-labeled profiles (221/566) either apposed DAT-immunoreactive terminals or also contained DAT. Of these 221 DOR-labeled profiles, 13% were axon terminals containing DAT and 15% were dendritic spines apposed to DAT-immunoreactive terminals. In contrast, 70% were morphologically heterogeneous axon terminals and small axons apposed to DAT-immunoreactive terminals. Our results indicate that DOR agonists in the AcbSh can directly modulate the release of dopamine, as well as postsynaptic responses in spiny neurons that receive dopaminergic input, but act principally to control the presynaptic secretion of other neurotransmitters whose release may influence or be influenced by extracellular dopamine. Thus, while opiates and psychostimulants mainly have differential sites of action, cross-sensitization of their addictive properties may occur through common neuronal targets.


Subject(s)
Carrier Proteins/physiology , Central Nervous System Stimulants/pharmacology , Dopamine/metabolism , Membrane Glycoproteins , Membrane Transport Proteins , Narcotics/pharmacology , Nerve Tissue Proteins , Nucleus Accumbens/metabolism , Receptors, Opioid, delta/physiology , Animals , Antibodies, Monoclonal , Axons/physiology , Axons/ultrastructure , Dendrites/physiology , Dendrites/ultrastructure , Dopamine Plasma Membrane Transport Proteins , Immunoenzyme Techniques , Immunohistochemistry , Male , Microscopy, Electron , Nerve Endings/physiology , Nerve Endings/ultrastructure , Nucleus Accumbens/drug effects , Rats , Rats, Sprague-Dawley , Receptors, Opioid, delta/drug effects , Receptors, Presynaptic/drug effects
7.
J Neurosci ; 19(5): 1804-13, 1999 Mar 01.
Article in English | MEDLINE | ID: mdl-10024364

ABSTRACT

The nucleus accumbens (Acb) is prominently involved in the aversive behavioral aspects of kappa-opioid receptor (KOR) agonists, including its endogenous ligand dynorphin (Dyn). We examined the ultrastructural immunoperoxidase localization of KOR and immunogold labeling of Dyn to determine the major cellular sites for KOR activation in this region. Of 851 KOR-labeled structures sampled from a total area of 10,457 microm2, 63% were small axons and morphologically heterogenous axon terminals, 31% of which apposed Dyn-labeled terminals or also contained Dyn. Sixty-eight percent of the KOR-containing axon terminals formed punctate-symmetric or appositional contacts with unlabeled dendrites and spines, many of which received convergent input from terminals that formed asymmetric synapses. Excitatory-type terminals that formed asymmetric synapses with dendritic spines comprised 21% of the KOR-immunoreactive profiles. Dendritic spines within the neuropil were the major nonaxonal structures that contained KOR immunoreactivity. These spines also received excitatory-type synapses from unlabeled terminals and were apposed by Dyn-containing terminals. These results provide ultrastructural evidence that in the Acb shell (AcbSh), KOR agonists play a primary role in regulating the presynaptic release of Dyn and other neuromodulators that influence the output of spiny neurons via changes in the presynaptic release of or the postsynaptic responses to excitatory amino acids. The cellular distribution of KOR complements those described previously for the reward-associated mu- and delta-opioid receptors in the Acb shell.


Subject(s)
Dynorphins/metabolism , Nucleus Accumbens/metabolism , Receptors, Opioid, kappa/metabolism , Animals , Axons/metabolism , Dendrites/metabolism , Male , Microscopy, Immunoelectron , Neuroglia/metabolism , Presynaptic Terminals/metabolism , Rats , Rats, Sprague-Dawley , Synapses/metabolism
8.
J Neurosci ; 18(19): 8003-15, 1998 Oct 01.
Article in English | MEDLINE | ID: mdl-9742167

ABSTRACT

GABAergic neurons in the ventral tegmental area (VTA) play a primary role in local inhibition of mesocorticolimbic dopamine (DA) neurons but are not physiologically or anatomically well characterized. We used in vivo extracellular and intracellular recordings in the rat VTA to identify a homogeneous population of neurons that were distinguished from DA neurons by their rapid-firing, nonbursting activity (19.1 +/- 1.4 Hz), short-duration action potentials (310 +/- 10 microseconds), EPSP-dependent spontaneous spikes, and lack of spike accommodation to depolarizing current pulses. These non-DA neurons were activated both antidromically and orthodromically by stimulation of the internal capsule (IC; conduction velocity, 2.4 +/- 0.2 m/sec; refractory period, 0.6 +/- 0.1 msec) and were inhibited by stimulation of the nucleus accumbens septi (NAcc). Their firing rate was moderately reduced, and their IC-driven activity was suppressed by microelectrophoretic application or systemic administration of NMDA receptor antagonists. VTA non-DA neurons were recorded intracellularly and showed relatively depolarized resting membrane potentials (-61.9 +/- 1.8 mV) and small action potentials (68.3 +/- 2.1 mV). They were injected with neurobiotin and shown by light microscopic immunocytochemistry to be multipolar cells and by electron microscopy to contain GABA but not the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH). Neurobiotin-filled dendrites containing GABA received asymmetric excitatory-type synapses from unlabeled terminals and symmetric synapses from terminals that also contained GABA. These findings indicate that VTA non-DA neurons are GABAergic, project to the cortex, and are controlled, in part, by a physiologically relevant NMDA receptor-mediated input from cortical structures and by GABAergic inhibition.


Subject(s)
Neurons, Afferent/physiology , Neurons, Efferent/physiology , Ventral Tegmental Area/cytology , gamma-Aminobutyric Acid/physiology , 2-Amino-5-phosphonovalerate/pharmacology , Action Potentials/drug effects , Action Potentials/physiology , Animals , Biotin/analogs & derivatives , Dopamine/physiology , Electrophysiology , Excitatory Amino Acid Antagonists/pharmacology , Extracellular Space/chemistry , Extracellular Space/enzymology , Male , Microscopy, Electron , Neural Inhibition/physiology , Neurons, Afferent/chemistry , Neurons, Afferent/ultrastructure , Neurons, Efferent/chemistry , Neurons, Efferent/ultrastructure , Nucleus Accumbens/cytology , Rats , Rats, Sprague-Dawley , Receptors, N-Methyl-D-Aspartate/analysis , Synapses/chemistry , Synapses/enzymology , Synapses/ultrastructure , Tyrosine 3-Monooxygenase/analysis , gamma-Aminobutyric Acid/analysis
9.
J Neurosci ; 18(5): 1923-33, 1998 Mar 01.
Article in English | MEDLINE | ID: mdl-9465017

ABSTRACT

The shell compartment of the nucleus accumbens (AcbSh) is prominently involved in the rewarding aspects of delta-opioid receptor (DOR) agonists, including one of its putative endogenous ligands, Met5-enkephalin (Enk). We examined the ultrastructural immunocytochemical localization of an antipeptide DOR antiserum and an antibody against Enk to determine the major cellular sites for DOR activation and the spatial relationship between DOR and Enk in this region. Sixty percent of DOR-immunoreactive profiles were axon terminals and small unmyelinated axons, whereas the remainder were mainly dendrites and dendritic spines. In axons and terminals, DOR labeling was distributed along plasma and vesicular membranes. DOR-containing terminals were mainly large and primarily formed symmetric synapses or occasionally asymmetric synapses. DOR immunoreactivity also was associated with terminals that were small and formed punctate symmetric or nonrecognizable synapses. Dual immunoperoxidase and immunogold labeling showed that 35% of DOR-labeled axons apposed other terminals that contained Enk. In addition, 25% of the DOR-labeled terminals contained Enk. Thirty-five percent of DOR labeling was observed within dendrites and dendritic spines. DOR-labeled spines showed intense immunoreactivity within asymmetric postsynaptic junctions, which were formed by terminals that lacked Enk immunoreactivity. DOR-labeled spines, however, were apposed to Enk-containing terminals in 13% of all associations between dually labeled profiles. These results provide ultrastructural evidence that activation of DOR in the AcbSh is primarily involved in modulating the presynaptic release of mainly inhibitory, but also excitatory, neurotransmitters. These data also suggest that DOR plays a role in determining the postsynaptic response to excitatory afferents.


Subject(s)
Enkephalin, Methionine/metabolism , Nucleus Accumbens/metabolism , Receptors, Opioid, delta/metabolism , Animals , Dendrites/metabolism , Dendrites/ultrastructure , Immunohistochemistry , Male , Microscopy, Electron , Nucleus Accumbens/cytology , Nucleus Accumbens/ultrastructure , Presynaptic Terminals/metabolism , Presynaptic Terminals/ultrastructure , Rats , Rats, Sprague-Dawley
10.
J Neurosci ; 17(12): 4839-48, 1997 Jun 15.
Article in English | MEDLINE | ID: mdl-9169542

ABSTRACT

The effectiveness of NMDA antagonists in modulating the motor and motivational effects of opiates is attributed, in part, to functional associations involving NMDA receptors and micro-opioid receptors (MORs) in the shell of the nucleus accumbens (Acb). To determine the subcellular sites for potential functional interactions between opiate ligands and NMDA receptors in this region, we examined the ultrastructural localization of antipeptide antisera against MOR and the R1 subunit of the NMDA receptor in the Acb shell of the adult rat brain. MOR-like immunoreactivity (MOR-LI) was seen primarily in dendrites, whereas NMDAR1-like immunoreactivity (NMDAR1-LI) was detected more often in axon terminals forming asymmetric synapses. In these profiles, MOR labeling was localized mainly to extrasynaptic plasma membranes, whereas NMDAR1-LI was associated with both synaptic and extrasynaptic sites. Of 307 MOR-labeled processes, 17.9% of the dendrites and 9.4% of the axon terminals also contained NMDAR1-LI. In addition, 24.7% of the dendrites containing only MOR-LI were apposed to NMDAR1-labeled axons or terminals. We conclude that in the shell of the Acb, the output of single neurons can be dually modulated by (1) activation of MOR and NMDA receptors in the same dendrites or (2) combined activation of presynaptic NMDA receptors in afferents contacting dendrites containing MOR. In addition, the colocalization of MOR and NMDAR1 in certain axon terminals in the Acb suggests their dual involvement in the presynaptic release of neurotransmitters in this region.


Subject(s)
Axons/ultrastructure , Dendrites/ultrastructure , Nerve Endings/ultrastructure , Neurons/ultrastructure , Nucleus Accumbens/ultrastructure , Receptors, N-Methyl-D-Aspartate/analysis , Receptors, Opioid, mu/analysis , Animals , Cell Membrane/ultrastructure , Male , Microscopy, Immunoelectron , Neuroglia/ultrastructure , Rats , Rats, Sprague-Dawley
11.
J Neurosci ; 17(7): 2585-94, 1997 Apr 01.
Article in English | MEDLINE | ID: mdl-9065518

ABSTRACT

The activation of mu-opioid receptors in the nucleus accumbens (Acb) produces changes in locomotor and rewarding responses that are believed to involve neurons, including local gamma-aminobutyric acid (GABA)ergic neurons. We combined immunogold-silver detection of an antipeptide antiserum against the cloned mu-opioid receptor (MOR) and immunoperoxidase labeling of an antibody against GABA to determine the cellular basis for the proposed opioid modulation of GABAergic neurons in the rat Acb. MOR-like immunoreactivity (MOR-LI) was localized prominently to plasma membranes of neurons having morphological features of both spiny and aspiny cells, many of which contained GABA. Of 351 examples of profiles that contained MOR-LI and GABA labeling, 65% were dendrites. In these dendrites, MOR-LI was seen mainly along extrasynaptic portions of the plasma membrane apposed to unlabeled terminals and/or glial processes. Dually labeled dendrites often received convergent input from GABAergic terminals and/or from unlabeled terminals forming asymmetric excitatory-type synapses. Of all profiles that contained both MOR and GABA immunoreactivity, 28% were axon terminals. MOR-containing GABAergic terminals and terminals separately labeled for MOR or GABA formed synapses with unlabeled dendrites and also with dendrites containing MOR or GABA. Our results indicate that MOR agonists could modulate the activity of GABA neurons in the Acb via receptors located mainly at extrasynaptic sites on dendritic plasma membranes. MOR ligands also could alter the release of GABA onto target dendrites that contain GABA and/or respond to opiate stimulation.


Subject(s)
Axons/ultrastructure , Neurons/cytology , Nucleus Accumbens/cytology , Receptors, Opioid, mu/analysis , Synapses/ultrastructure , Animals , COS Cells , Cell Membrane/ultrastructure , Immunohistochemistry , Male , Microscopy, Immunoelectron , Nerve Endings/ultrastructure , Neurons/ultrastructure , Nucleus Accumbens/ultrastructure , Rats , Rats, Sprague-Dawley , Receptors, Opioid, mu/immunology , Recombinant Proteins/immunology , Transfection
12.
J Neurosci ; 16(13): 4162-73, 1996 Jul 01.
Article in English | MEDLINE | ID: mdl-8753878

ABSTRACT

mu-Opioid receptors and their endogenous ligands, including Leu5-enkephalin (LE), are distributed abundantly in the nucleus accumbens (NAC), a region implicated in mechanisms of opiate reinforcement. We used immunoperoxidase and/or immunogold-silver methods to define ultrastructural sites for functions ascribed to mu-opioid receptors and potential sites for activation by LE in the NAC. An antipeptide antibody raised against an 18 amino acid sequence of the cloned mu-opioid receptor (MOR) C terminus showed that MOR-like immunoreactivity (MOR-LI) was localized predominantly to extrasynaptic sites along neuronal plasma membranes. The majority of neuronal profiles containing MOR-LI were dendrites and dendritic spines. The dendritic plasma membranes immunolabeled for MOR were near sites of synaptic input from LE-labeled terminals and other unlabeled terminals forming either inhibitory or excitatory type synapses. Unmyelinated axons and axon terminals were also intensely but less frequently immunoreactive for MOR. Observed sites for potential axonal associations with LE included coexistence of MOR and LE within the same terminal, as well as close appositions between differentially labeled axons. Astrocytic processes rarely contained detectable MOR-LI, but also were sometimes observed in apposition to LE-labeled terminals. We conclude that in the rat NAC, MOR is localized prominently to extrasynaptic neuronal and more rarely to glial plasma membranes that are readily accessible to released LE and possibly other opioid peptides and opiate drugs. The close affiliation of MOR with spines receiving excitatory synapses and dendrites receiving inhibitory synapses provides the first direct morphological evidence that MOR selectively modulates postsynaptic responses to cortical and other afferents.


Subject(s)
Enkephalin, Leucine/metabolism , Nucleus Accumbens/metabolism , Nucleus Accumbens/ultrastructure , Receptors, Opioid, mu/metabolism , Animals , Astrocytes/metabolism , Axons/metabolism , Cell Membrane/metabolism , Dendrites/metabolism , Immunohistochemistry , Male , Nerve Endings/metabolism , Rats , Rats, Sprague-Dawley , Tissue Distribution
13.
Brain Res ; 700(1-2): 25-39, 1995 Nov 27.
Article in English | MEDLINE | ID: mdl-8624718

ABSTRACT

The insular cortex has been implicated in the reinforcing properties of opiates as well as in the integration of responses to sensory-motor stimulation. Moreover, the delta-opioid receptor (DOR) and the endogenous opioid ligand, Met5-enkephalin (ENK) are known to be prominently distributed in insular limbic cortex. To examine the anatomical sites for opioid activation of DOR in rat insular cortex, we used immunoperoxidase for detection of an antiserum raised against a peptide sequence unique to the DOR alone, and in combination with immunogold-silver labeling for ENK. Light microscopy showed intense DOR-like immunoreactivity (DOR-LI) in pyramidal cells and interneurons in deep laminae, and in varicose processes in both superficial and deep layers of the insular cortex. Ultrastructural analysis of layers V and VI in insular cortex showed that the most prominent immunoperoxidase labeling for DOR was in dendrites. This labeling was associated with asymmetric excitatory-type junctions postsynaptic to unlabeled terminals. Dendritic DOR-LI was also distributed along selective portions of non-synaptic plasma membranes and subsurface organelles. In dually labeled sections, dendrites containing DOR-LI sometimes received synaptic input from ENK-labeled terminals or more infrequently colocalized with ENK. Other axon terminals were exclusively immunolabeled for DOR or more rarely contained both DOR and ENK immunoreactivity. Within labeled axon terminals, distinct segments of the plasma membrane and membranes of immediately adjacent synaptic vesicles showed the largest accumulation of the peroxidase reaction product for DOR. These results indicate that in rat insular cortex DOR is primarily heteroreceptive, but also serves an autoreceptive function on certain ENK-containing neurons. Our results also provide the first ultrastructural evidence that in rat insular cortex endogenous opioids interact through the DOR (1) to modulate the postsynaptic responses to other excitatory afferents and (2) to presynaptically regulate the release of other neurotransmitters. The modulatory actions on both ENK-containing and non-ENK-containing neurons may contribute significantly to the reinforcing properties of exogenous opiates acting on the DOR in limbic cortex.


Subject(s)
Cerebral Cortex/chemistry , Enkephalin, Methionine/analysis , Neuropeptides/analysis , Peptide Fragments/analysis , Receptors, Opioid, delta/analysis , Animals , Axons/chemistry , Cerebral Cortex/ultrastructure , Dendrites/chemistry , Immunoenzyme Techniques , Male , Microscopy, Electron , Myelin Sheath , Nerve Endings/chemistry , Rats , Rats, Sprague-Dawley
14.
J Neurosci ; 15(9): 5976-88, 1995 Sep.
Article in English | MEDLINE | ID: mdl-7666182

ABSTRACT

Opioid peptides, Met5- and Leu5-enkephalin, are known endogenous ligands for the delta-opioid receptor (DOR) associated with opioid analgesia at the spinal level. To determine the cellular sites for DOR-mediated actions, we examined the ultrastructural localization of DOR and Met5-enkephalin (ME) in the spinal cord by combining immunoperoxidase and immunogold-silver labeling for antibodies against DOR and ME, respectively. Antibodies for DOR localization were raised in guinea pig against peptide 34-47 (p34), an amino acid sequence within the extracellular N-terminus of the DOR recently cloned from mouse neuroblastoma glioma (NG-108) cells. Selective immunoperoxidase labeling for DOR was detected by light microscopy in NG-108 cells and in the lamina I and II of the dorsal horn of the spinal cord (C2-C4). Electron microscopy of these spinal laminae revealed that the majority of the punctate varicosities seen by light microscopy were axon terminals. delta-opioid receptor-like immunoreactivity (DOR-LI) in axon terminals was most prominently associated with large dense core vesicles, and sometimes seen along the membranes of small clear vesicles and segments of the plasmalemma. A semiquantitative analysis of dually labeled sections revealed that of the terminals showing DOR-LI, 23/102 (23%) also contained Met5-enkephalin-like immunoreactivity (ME-LI). Conversely, 23/35 (66%) of the terminals showing ME-LI also showed DOR-LI. In addition to the presynaptic localization, selective postsynaptic densities within dendrites were also occasionally (9%) immunolabeled for the opioid receptor. These results provide the first ultrastructural evidence that DOR may serve autoreceptor functions on ME terminals as well as presynaptic modulation of other transmitters in the dorsal horn of the rat spinal cord. Additionally, the vesicular localization of DOR-LI in axon terminals suggests the involvement of these organelles in the transport of the receptors to the plasma membrane.


Subject(s)
Axons/metabolism , Enkephalin, Methionine/metabolism , Nerve Endings/metabolism , Presynaptic Terminals/metabolism , Receptors, Opioid, delta/metabolism , Spinal Cord/metabolism , Amino Acid Sequence , Animals , Dendrites/metabolism , Immune Sera/immunology , Immunoenzyme Techniques , Immunohistochemistry , Male , Molecular Sequence Data , Neck , Peptide Fragments/genetics , Peptide Fragments/immunology , Rats , Rats, Sprague-Dawley , Receptors, Opioid, delta/genetics , Spinal Cord/ultrastructure , Tissue Distribution
15.
Pharmacol Biochem Behav ; 45(4): 759-63, 1993 Aug.
Article in English | MEDLINE | ID: mdl-8415815

ABSTRACT

Pretreatment with the serotonin-3 (5-HT3) antagonist racemic (+/-)-Zacopride hydrochloride (ZAC, 0.1 mg/kg, IP) has been previously found to completely abolish the locomotor activity induced by cocaine (10 mg/kg, IP). To determine if this effect was mediated by fluctuations in the extracellular levels of forebrain dopamine (DA), we examined the ability of ZAC to attenuate cocaine-induced increases in extracellular DA levels. Microdialysis samples were collected from the nucleus accumbens region (NAS) of awake, male, Sprague-Dawley rats. ZAC treatment alone (0.1 mg/kg, IP) did not alter DA levels relative to baseline. However, this dose of ZAC given 1 h prior to cocaine challenge (10 mg/kg, IP) caused a 27% reduction in the peak level of extracellular DA produced by cocaine, relative to saline-pretreated control animals. These results suggest that the ability of ZAC to attenuate cocaine-induced increases in extracellular DA levels may contribute to ZAC's ability to suppress cocaine-induced locomotor activity in the rat. However, additional neurochemical mechanisms are likely to be important in mediating the robust behavioral effects previously reported.


Subject(s)
Benzamides/pharmacology , Bridged Bicyclo Compounds, Heterocyclic , Bridged Bicyclo Compounds/pharmacology , Cocaine/antagonists & inhibitors , Dopamine/metabolism , Nucleus Accumbens/metabolism , Serotonin Antagonists/pharmacology , 3,4-Dihydroxyphenylacetic Acid/metabolism , Animals , Chromatography, High Pressure Liquid , Cocaine/pharmacology , Dialysis , Extracellular Space/drug effects , Extracellular Space/metabolism , Homovanillic Acid/metabolism , Male , Motor Activity/drug effects , Nucleus Accumbens/drug effects , Prosencephalon/drug effects , Prosencephalon/metabolism , Rats , Rats, Sprague-Dawley
16.
Pharmacol Biochem Behav ; 43(3): 871-9, 1992 Nov.
Article in English | MEDLINE | ID: mdl-1448481

ABSTRACT

We report results in rats pretreated with (+/-)-zacopride (0.03 mg/kg, IP), ICS 205-930 (0.1 mg/kg, IP), and MDL 72222 (1.0 mg/kg, IP) 15 min before challenge with (-)-cocaine (10.0 mg/kg, IP). At a dose of 10 micrograms/kg, zacopride significantly inhibited (approximately 50%) cocaine-induced locomotion. We also investigated whether or not 5-hydroxytryptamine3 (5-HT3) antagonists block the cocaine binding site on the dopamine transporter and/or affect the ability of dopamine to regulate this binding site. In well-washed striatal membranes, neither zacopride nor ICS 205-930 (10(-9)-10(-5) M) inhibited [3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane ([3H]WIN 35,428) (0.3 nM) binding. Furthermore, neither of these compounds affected the ability of dopamine to block WIN 35,428 binding. To determine if 5-HT is required for the 5-HT3 antagonist effect, we examined the interaction between cocaine and zacopride in rats pretreated with p-chlorophenylalanine (PCPA) (3 days x 100 mg/kg/day). PCPA pretreatment shifted the cocaine dose-response curve to the right and blocked the ability of zacopride to reverse cocaine-induced activity.


Subject(s)
Bridged Bicyclo Compounds, Heterocyclic , Cocaine/antagonists & inhibitors , Fenclonine/pharmacology , Motor Activity/drug effects , Selective Serotonin Reuptake Inhibitors/pharmacology , Animals , Benzamides/pharmacology , Bridged Bicyclo Compounds/pharmacology , Cocaine/analogs & derivatives , Cocaine/metabolism , Dose-Response Relationship, Drug , Indoles/pharmacology , Male , Rats , Rats, Sprague-Dawley , Receptors, Serotonin/metabolism , Serotonin Antagonists/pharmacology , Tropanes/pharmacology , Tropisetron
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