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Vestn Ross Akad Med Nauk ; (11-12): 10-3, 1992.
Article in Russian | MEDLINE | ID: mdl-1284212

ABSTRACT

HIV strains were isolated from HIV-infected patients and AIDS patients in CIS. A total of 81 HIV isolates were obtained. The isolates were identified by using immunofluorescence and enzyme immunoassay, by determining the activity of reverse transcriptase, immunoblot, electron microscopy, polymerase chain reaction. Of the 81 isolates 79 were HIV-1 and 2 HIV-2. The strains differed in their infectivity, the kinetics of virus antigen accumulation, and the spectrum of susceptible cell lines. The viruses isolated may be assigned as two groups: high and low infective. The biological properties of the national HIV isolates were shown to be similar to the prototype HIV strains isolated elsewhere.


Subject(s)
Acquired Immunodeficiency Syndrome/microbiology , HIV Infections/microbiology , HIV-1/isolation & purification , HIV-2/isolation & purification , Commonwealth of Independent States , Fluorescent Antibody Technique , HIV Antibodies/blood , HIV-1/classification , HIV-1/immunology , HIV-2/classification , HIV-2/immunology , Humans , Immunoenzyme Techniques , Lymphocytes/microbiology , Microscopy, Electron , Polymerase Chain Reaction , RNA-Directed DNA Polymerase/analysis
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