ABSTRACT
In vitro antiviral effect of myramistin on influenza virus (MDCK cell culture) was studied. The drug showed significant dose-dependent antiviral activity against the virus. When used prophylactically (1 hour before exposure to the virus) in subtoxic doses, myramistin was effective in inhibiting replication of the influenza virus [strains A/Aichi/2/68 (H3N2) and A/Chicken/Suzdalka/Nov-11/2005 (H5N1)]. In urgent prophylaxis (1 hour after exposure to the virus) the protective effect was less pronounced, especially when the contamination dose was high. When the drug was added 12 hours after exposure to the virus, it had no protective effect on the MDSK cell monolayer. The prospects of the myramistin use as a prophylactic agent in grippe are discussed.
Subject(s)
Antiviral Agents/pharmacology , Benzalkonium Compounds/pharmacology , Influenza A Virus, H3N2 Subtype/drug effects , Influenza A Virus, H5N1 Subtype/drug effects , Animals , Cell Line , Dogs , Humans , Influenza A Virus, H3N2 Subtype/physiology , Influenza A Virus, H5N1 Subtype/physiology , Virology/methods , Virus ReplicationABSTRACT
The study was aimed at in vitro investigation of the Myramistin antiviral activity against the measles and mumps viruses in the Vero cell culture. The experiments with addition of myramistin simultaneously or at various periods after inoculation of the monolayer by the measles virus (Edmonson strain) or mumps virus (PetroNov/03 strain) revealed pronounced dose-dependent antiviral effect of the drug. It was shown that for prevention of replication of the measles and mumps viruses the optimal concentrations were 0.05 to 0.005%. The prospects of myramistin use as a prophylactic agent for infections caused by the measles and mumps viruses are discussed.
Subject(s)
Antiviral Agents/pharmacology , Benzalkonium Compounds/pharmacology , Measles virus/drug effects , Mumps virus/drug effects , Animals , Chlorocebus aethiops , Dose-Response Relationship, Drug , Measles virus/physiology , Microbial Sensitivity Tests , Mumps virus/physiology , Vero Cells , Virus Replication/drug effectsABSTRACT
To elucidate the combined effect of surfactants and antituberculous drugs, the surfactant myrastimine was tested. Two series of sputum cultures with multidrug-resistant Mycobacterium tuberculosis (MBT) strains were made. The experimental results confirmed that myramistine noticeably enhanced the sensitivity of multidrug-resistant MBT strains to antituberculous agents. The use of endobronchial and intracavernous myramistine in combination with antituberculous agents to patients with destructive pulmonary tuberculosis caused by multidrug-resistant MBT strains reduced the latter's resistance to antibiotics, cured tubendobronchitis in most patients, created conditions for obliteration of the cavernous cavity. Positive changes are noted in the first 4 months after the initiation of therapy, which can prepare patients for surgical treatment more rapidly.
Subject(s)
Antitubercular Agents/therapeutic use , Tuberculosis, Multidrug-Resistant/drug therapy , Tuberculosis, Pulmonary/drug therapy , Adult , Drug Resistance, Multiple, Bacterial , Humans , Russia/epidemiology , Tuberculosis, Multidrug-Resistant/epidemiology , Tuberculosis, Pulmonary/epidemiologySubject(s)
Aniline Compounds/toxicity , Water Pollutants, Chemical/toxicity , Water Pollutants/toxicity , Water Supply , Aniline Compounds/analysis , Animals , Female , Fetal Death/chemically induced , Hematopoiesis/drug effects , Male , Maximum Allowable Concentration , Mice , Organ Size/drug effects , Pregnancy , Rats , Time FactorsSubject(s)
Amino Acids/toxicity , Oxamic Acid/toxicity , Surface-Active Agents/toxicity , Water Pollutants, Chemical/toxicity , Water Pollutants/toxicity , Animals , Dose-Response Relationship, Drug , Female , Lethal Dose 50 , Male , Maximum Allowable Concentration , Mice , Oxamic Acid/analogs & derivatives , Rats , Time FactorsABSTRACT
The effect of prolonged (for 1 1/2 month) action of ethylene glycol and fatty acids ester on the bodily function was studied in experiments on rats by means of a complex of techniques. The threshold dose determined in a subacute experiment amounted to 1.8625 g/kg. The cumulative effect of the preparation is evaluated as little and medium cumulative.
Subject(s)
Ethylene Glycols/pharmacology , Fatty Acids/pharmacology , Animals , Blood/drug effects , Dose-Response Relationship, Drug , Esters , Female , Male , Ovum , Rats , Time FactorsABSTRACT
In tests staged on anesthetized cats the effects of reserpine, isobarine and dopegit upon the resistance of cerebral vessels were studied resistographically. In 60--120 minutes after their introduction these drugs raise the resistance of intracranial vessels, inhibiting their reaction to electric stimulation of sympathic nerves and bringing down at the same time the total arterial pressure. An inference is drawn to the effect that the mounting perfusion pressure in the cerebral vessels following the action of reserpine and isobarine occurs as a result of their alpha-adrenoreceptors stimulation with norepinephrine that is intensively secreted from the nerve endings. Dopegit exerts an analogous action through formation of alpha-methyl-norepinephrine, which produces, like norepinephrine, an effect on the cerebral vessels.
