[Results of the experiment with extended exposure of microorganisms in open space].

Purpose of the experiment was to define the hypothesized margins of phenotype adaptation and genotype modifications of bacteria-fungi associations that form the typical microbiota on space vehicle materials. The fundamental questions to answer were whether the selected test-cultures would remain vital after extended (comparable to the Mars mission duration) exposure in open space as part of microbesstructural material model systems, and how the space factors would affect microorganisms in the context of eco-safety of space vehicles and planetary quarantine precautions. The first extended (1.5 years) exposure of the model systems outside of the International space station proved survivability of bacterial and microfungal spores in open space. Ultrastructures of eukaryotic and prokaryotic organisms were found altered, and so were their biological properties.

[Main results of the Biorisk experiment on the International Space Station].

To get better appreciation of the margins of phenotypic adaptation and genotypic changes in bacteria-fungi associations within the typical microbiota residing on structural materials of space-flown equipment, developed were a program and hardware for a series of experiments under the general name BIORISK. Protocol of each experimental cycle is based on the well-proven method of exposure of "passive" samples of materials (Biorisk-KM), microorganisms-materials systems inside the ISS service module (Biorisk-MSV), and microorganisms-materials systems on the outside of the ISS SM (Biorisk-MSN). Each six months the samples are returned to the laboratory in conjunction with crew rotation. Already the first in-hand data from the experiment point to the dramatic effect of space flight on growth, reproduction, and biological properties of test microbes and fungi. Thus, the activity of enzymes that characterize the pathogenic potential (RNA-ase and DNA-ase), and resistance of microorganisms to aseptic agents were found increased.

[Studies on the growth and reproduction of bacterial communities on structural materials of the international space station].

Probability of microbial growth and reproduction on the ISS interior and equipment materials varying in chemical composition was studied with the strains of Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus saprophyticus, Pseudomonas putida etc. sampled from the ISS environment. Controls were ground reference strains of same bacterial species. Based on our results, some of the microorganisms are able to survive and proliferate on structural materials; the ability was greater in space isolates as compared with their ground analogs. The greatest ability to grow and proliferate on materials was demonstrated by Bacillus subtilis.