Alternations of Fertility Parameters by Graded Dose of Inorganic Arsenic in Adult Male White Pekin Ducks.

A significant health issue, reproductive toxicity is mostly linked to exposure to various environmental heavy metals. A pervasive toxin that occurs naturally in the environment is arsenic (As). This research was done to determine the effects of various doses of inorganic As supplements on the reproductive organs of adult male white Pekin ducks. A total of 240 numbers of 14-days-old male white Pekin ducks were weighed and randomly assigned into 4 experimental groups with six replicates (10 ducklings in each replicate). The experimental groups were as follows: (T-1) basal diet along with normal drinking water (control group); (T-2 to T-4) basal diet along with As in the form of sodium-meta-arsenite at 7, 14, and 28 ppm of drinking water respectively. The results showed reduction in body weight and testicular weight, disruption of spermatogenesis, reduction in follicular-stimulating hormone (FSH), leutinizing hormone (LH), and testosterone levels and histopathological alterations as compared to control. Additionally, there was not only a significant decrease in various antioxidant parameters in testis tissue, like catalase (CAT), reduced glutathione (GSH), super oxide dismutase (SOD), and ferric-reducing antioxidant power (FRAP), but also a significant increase in oxidative parameters of testis like lipid peroxidation (LPO), myloperoxidase (MPO), nitric oxide (NO), and super oxide anion radical (O2-) in As-treated groups, in comparison with T-1. A significantly higher level of As content in testis was observed in all the 3 As-treated groups, with highest level recorded in T-4 birds. Besides that, there was upregulation of nuclear factor kappa B (NF-κB), heat shock proteins (Hsps) and pro-inflammatory cytokines like interlukin (IL) series, i.e., IL-2, IL-6, IL-18, IL-1ß and tumor necrosis factor- α (TNF-α) levels, whereas anti-inflammatory parameters like IL-4 and IL-10 levels showed downregulation in testis of As-treated groups. Together, these findings provide deeper understandings of the roles played by oxidative stress, NF-κB and Hsps in the progression of testicular injury, which may help to explain how the As induced male sterility, in ducks, due to exposure.

Hepatorenal Toxicity of Inorganic Arsenic in White Pekin Ducks and Its Amelioration by Using Ginger.

The toxic metalloid arsenic is known to cause liver and kidney injury in many humans and animals. The goal of this paper was to exemplify the antagonism of ginger against arsenic (As)-induced hepato-renal toxicity. In addition, the pathways Nrf2/Keap1 and NF/κB were studied to reveal the molecular mechanism of the stress. One hundred twenty 7-day-old White Pekin ducks were randomly allocated into five groups, having 24 birds in each. Each group contained three replicates having 8 birds in each replicate and maintained for 90 days. The groups were as follows: T-1 [control-basal diet with normal water], T-2 [T1 + As at 28 ppm/L of water], T-3 [T2 + ginger powder at 100 mg/kg feed], T-4 [T2 + ginger powder at 300 mg/kg feed], and T-5 [T2 + ginger powder at 1 g/kg feed]. It was observed that there was a significant increase in oxidative parameters whereas a significant decrease in antioxidant parameters in hepato-renal tissues in T-2. The exposure to As not only decreased the mRNA expression of antioxidant parameters like Nrf2, SOD-1, CAT, GPX, and HO-1and anti-inflammatory markers like IL-4 and IL-10 but also increased the m-RNA expression of NF-κB, Keap-1 and pro-inflammatory markers like IL-2, Il-6, IL-18, IL-1ß, and TNF-α. There was also an accumulation of As in hepatic and renal tissue, confirmed by residual analysis of these tissues. By correlating the above parameters, As at 28 ppm showed significant toxic effects, and ginger powder at 1 g/kg feed effectively counteracted the toxic effects of As in ducks.