ABSTRACT
Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen and causes endophthalmitis, liver abscess, osteomyelitis, meningitis, and necrotizing soft tissue infections in both immunodeficient and healthy people. The acquisition of the antibiotic resistance genes of hvKp has become an emerging concern throughout the globe. In this study, a total of 74 K. pneumoniae isolates were collected and identified by VITEK2 and blaSHV gene amplification. Out of these, 18.91% (14/74) isolates were identified as hvKp by both phenotypic string test and genotypic iucA PCR amplification. The antibiotic susceptibility revealed that 57.14% (8/14) isolates were multidrug-resistant (MDR) and 35.71% (5/14) isolates were extremely drug-resistant (XDR). All the isolates were resistant to ß-lactam, ß-lactamase + inhibitor groups of antibiotics, and the least resistance to colistin. Of 14 hvKp isolates, all isolates are positive for iroB (100%), followed by iutA (92.85%), peg344 (85.71%), rmpA (57.14%), and magA (21.42%) genes. Among serotypes, K1 was the most prevalent serotype 21.4% (3/14), followed by K5 14.3% (2/14). The most common carbapenemase gene was blaOXA-48 (78.57%) followed by blaNDM (14.28%) and blaKPC (14.28%) which co-carried multiple resistance genes such as blaSHV (100%), blaCTX-M (92.85%), and blaTEM (78.57%). About 92.85% (13/14) of hvKp isolates were strong biofilm producers, while one isolate (hvKp 10) was the only moderate biofilm producer. The (GTG)5-PCR molecular typing method revealed high diversity among the hvKp isolates in the tertiary care hospital. Our findings suggest that MDR-hvKp is an emerging pathogen and a challenge for clinical practice. In order to avoid hvKp strain outbreaks in hospital settings, robust infection control and effective surveillance should be implemented.
ABSTRACT
Objectives: The study was done to assess the implant-abutment interface static compressive. strength of three design types and implant-abutment connection failure style. Materials and Methods: The implants at 306 with respect to the y-axis were aligned using a stainless steel holding device. Twenty specimens from each system totaled 60 total. A unidirectional vertical piston in a computer-controlled universal testing machine (MTS 810) generated static compression loading until failure. Specimens were macroscopically examined for fracture of the screw and implant, abutment looseness, and longitudinal displacement. Analysis of variance was used to analyze the data (ANOVA). Result: The screw-vent system had a mean compressive strength of 335.6 22.7 psi for the Unipost system, 384.3 37.1 psi for the screw-vent system, and 245.3 25.4 psi for the ITI-1 piece abutment connection. Conclusion: The connection between the Unipost implant and abutment showed a statistically significant variation.
