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Appl Environ Microbiol ; 72(1): 963-7, 2006 Jan.
Article in English | MEDLINE | ID: mdl-16391146

ABSTRACT

The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.


Subject(s)
Bacillaceae/enzymology , Escherichia coli K12/enzymology , Methyltransferases/genetics , Methyltransferases/metabolism , Selenium Compounds/metabolism , Bacillaceae/genetics , Culture Media , Escherichia coli K12/drug effects , Escherichia coli K12/genetics , Organoselenium Compounds/metabolism , Selenic Acid , Volatilization
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