Intra-Annual Variation of Eddy Diffusion (k zz ) in the MLT, From SABER and SCIAMACHY Atomic Oxygen Climatologies.

Atomic oxygen (O) in the mesosphere and lower thermosphere (MLT) results from a balance between production via photo-dissociation in the lower thermosphere and chemical loss by recombination in the upper mesosphere. The transport of O downward from the lower thermosphere into the mesosphere is preferentially driven by the eddy diffusion process that results from dissipating gravity waves and instabilities. The motivation here is to probe the intra-annual variability of the eddy diffusion coefficient (k zz ) and eddy velocity in the MLT based on the climatology of the region, initially accomplished by Garcia and Solomon (1985, https://doi.org/10.1029/JD090iD02p03850). In the current study, the intra-annual cycle was divided into 26 two-week periods for each of three zones: the northern hemisphere (NH), southern hemisphere (SH), and equatorial (EQ). Both 16 years of SABER (2002-2018) and 10 years of SCIAMACHY (2002-2012) O density measurements, along with NRLMSIS® 2.0 were used for calculation of atomic oxygen eddy diffusion velocities and fluxes. Our prominent findings include a dominant annual oscillation below 87 km in the NH and SH zones, with a factor of 3-4 variation between winter and summer at 83 km, and a dominant semiannual oscillation at all altitudes in the EQ zone. The measured global average k zz at 96 km lacks the intra-annual variability of upper atmosphere density data deduced by Qian et al. (2009, https://doi.org/10.1029/2008JA013643). The very large seasonal (and hemispherical) variations in k zz and O densities are important to separate and isolate in satellite analysis and to incorporate in MLT models.

A photometer array for atmospheric gravity wave measurements on the Lower Atmosphere Ionosphere Coupling Experiment (LAICE) mission.

The photometer payload of the lower atmosphere ionosphere coupling experiment CubeSat mission will observe and characterize atmospheric gravity wave (AGW) propagation through the mesosphere/lower thermosphere region of Earth's atmosphere on a global scale. AGW characteristics will be measured via passive observation of airglow emission from atmospheric O2( b 1 Σ g + ) at 762.0 nm (O2A) and Herzberg I O2( A 3 Σ u + - X 3 Σ g - ) at 277.0 nm (O2HI) under nighttime conditions. The photometer payload consists of a seven-element array of photomultiplier tubes grouped into four channels, which will measure O2A intensity at two emission wavelengths, O2HI band intensity at a single emission wavelength, and ambient background intensity at 770.0 nm. AGW horizontal wavelength will be measured from O2A band airglow perturbations related to rotational temperature and density, while vertical wavelength will be determined from the phase relationship between the O2HI and O2A bands. Wave number and wave amplitude will be used to determine the extent of energy and momentum flux associated with the wave. This is important in understanding the global distribution of high frequency waves which carry the bulk of the influence of wave energy and momentum flux from lower altitudes into the mesosphere. This can only be measured from space by nadir viewing. To our knowledge, nadir viewing of mesospheric airglow to quantify intrinsic properties of gravity waves from space has not been performed to date.

The Ionospheric Connection Explorer Mission: Mission Goals and Design.

The Ionospheric Connection Explorer, or ICON, is a new NASA Explorer mission that will explore the boundary between Earth and space to understand the physical connection between our world and our space environment. This connection is made in the ionosphere, which has long been known to exhibit variability associated with the sun and solar wind. However, it has been recognized in the 21st century that equally significant changes in ionospheric conditions are apparently associated with energy and momentum propagating upward from our own atmosphere. ICON's goal is to weigh the competing impacts of these two drivers as they influence our space environment. Here we describe the specific science objectives that address this goal, as well as the means by which they will be achieved. The instruments selected, the overall performance requirements of the science payload and the operational requirements are also described. ICON's development began in 2013 and the mission is on track for launch in 2017. ICON is developed and managed by the Space Sciences Laboratory at the University of California, Berkeley, with key contributions from several partner institutions.

Extraction of motion parameters of gravity-wave structures from all-sky OH image sequences.

We describe the application of a motion model to OH images collected by CCD cameras during three nights in February and April 1995 (3 February, 1 April, and 4 April) at the StarFire Optical Range, New Mexico. We used the instrument to take broadband images of OH Meinel bands at an altitude of 87 km to record the footprints of dynamics created by acoustic gravity waves in the mesosphere. We used the motion model to extract the velocity of the gravity waves from the images. The results of a total of 181 observations with the motion model were compared with a total of 189 observations obtained by manual estimation. We used these results to extract the intrinsic properties of the gravity waves. The mean intrinsic velocity for the three nights under consideration was 61.5 +/- 17.0 m/s with the motion model and 61.1 +/- 23.3 m/s with manual estimation.

Imaging spectroscopy for two-dimensional characterization of auroral emissions.

A large throughput transmission spectrometer, with a grating on a prism as the diffraction element, has been developed to study altitude distributions of auroral emissions. The imaging spectrometer disperses spectrally in one dimension while spatial information is preserved in the orthogonal direction. The image is projected onto a CCD array detector. Image processing methods have been developed to calibrate for wavelength, uniform field, spectral sensitivity, curvature of field, and spatial mapping. Single images are processed to represent a measured signal brightness in a unit of Rayleighs/pixel, from which area integrations can be made for desired spatial-spectral resolution. System performance is ~1.5-nm resolution over a 450-nm bandwidth (420-870 nm). Two spectrometer systems of this design were operated simultaneously, one with additional optical instruments and an incoherent scatter radar at Sondrestrom, Greenland, and the other at Godhavn, Greenland, which lies 290 km to the northwest and nearly in the magnetic meridian of Sondrestrom. The developed system, calibration method, and examples of performance results are presented.

Localization of an RNA binding element of the iron responsive element binding protein within a proteolytic fragment containing iron coordination ligands.

The iron responsive element binding protein (IRE-BP) regulates iron storage and uptake in response to iron. This control results from the interaction of the IRE-BP with the iron responsive element (IRE), a conserved sequence/structure element located near the 5' end of all ferritin mRNAs and in the 3' UTR of transferrin receptor mRNAs. Proteolysis was used to probe for functional elements of the IRE-BP. Partial chymotrypsin digestion generates a simple digestion pattern yielding fragments of 68, 56, 41, and 30 kDa. The 68 and 30 kDa fragments are derived from a single cleavage at Trp623. Further cleavages of the 68 kDa polypeptide yield the 56 and 41 kDa peptides. A combination of UV-crosslinking and chymotrypsin digestion was used to localize an RNA binding element within the C-terminus of the 68 kDa fragment, between amino acid residues 480 and 623. This region includes cysteine residues 503 and 506 which have been shown to be required for iron-sulfur cluster assembly and for iron regulation of the IRE-BP. Proteolytic fragments of the IRE-BP that contain this RNA binding region can be crosslinked to the IRE but do not bind with high affinity, suggesting that elements within the IRE-BP, in addition to those located between residues 480 and 623, are required for high affinity binding to the IRE.

Hadamard spectroscopy with a two-dimensional detecting array.

In a conventional grating spectrograph consisting of a single entrance slit, a grating, and a multichannel (imaging) detector, considerable light throughput advantage can be realized by replacement of the single entrance slit with a mask. This replacement can yield a signal-to-noise ratio increase because of increased light collection over an extended area of the mask when compared with a single slit. The mask produces a spectrum on the detector, which is the convolution of the mask pattern and the spectral distribution of the light source. To retrieve the spectrum, the spectrum has to be inverted. In special cases in which emission spectra are superimposed on weak backgrounds, the signal-to-noise advantage is preserved through the inversion process. Thus this technique is valuable in the observation of light sources that are produced by atomic or molecular emissions such as aurora, airglow, some interstellar emission, or laboratory spectra. Considerable signal-to-noise advantages can also be realized when the background noise of the imaging detector is not negligible. The spectral mixing of the light from the mask on the detector causes high photon fluxes on the detector, which tend to swamp the detector noise. This is a particularly important advantage in the application of CCD's as detectors because they can have significant background noise. The technique was demonstrated by computer simulations and laboratory tests.

Characteristics of the interaction of the ferritin repressor protein with the iron-responsive element.

The iron-responsive regulation of ferritin mRNA translation is mediated by the specific interaction of the ferritin repressor protein (FRP) with the iron-responsive element (IRE), a highly conserved 28-nucleotide sequence located in the 5' untranslated region of ferritin mRNAs. The IRE alone is necessary and sufficient to confer repression of translation by FRP upon a heterologous message, chloramphenicol acetyltransferase, in an in vitro translation system. The activity of FRP is sensitive to iron in vivo. Cytoplasmic extracts of rabbit kidney cells show reduction of FRP activity when grown in the presence of iron, as detected by RNA band shift assay. Using a nitrocellulose filter binding assay to examine the interaction of FRP with the IRE in more detail, we find that purified FRP has a single high-affinity binding site for the IRE with a Kd of 20-50 pM. Hemin pretreatment decreases the total amount of FRP which can bind to the IRE. This effect is dependent on hemin concentration. Interestingly, the FRP which remains active at a given hemin concentration binds to the IRE with the same high affinity as untreated FRP. A variety of hemin concentrations were examined for their effect on preformed FRP/IRE complexes. All hemin concentrations tested resulted in rapid complex breakdown. The final amount of complex breakdown corresponds to the concentration of hemin present in the reaction. The effect of hemin on FRP activity suggests that a specific hemin binding site exists on FRP.

Space plasma physics: atmospheric emissions photometric imaging experiment.

The atmospheric emissions photometric imaging experiment was flown on Spacelab 1 to study faint natural and artificial atmospheric emission phenomena. The instrument imaged optical emission in the region 2000 to 7500 angstroms with a television system consisting of two optical channels, one wide-angle and one telephoto. A third optical channel imaged onto the photochathode of a microchannel plate photomultiplier tube that has 100 discrete anodes. A hand-held image intensifier camera with an objective grating permitted spectral analysis of the earth's airglow and the shuttle glow. Preliminary data show magnesium ion emission features in the lower ionosphere as well as the spececraft glow spectrum.

Fabry-Perot-interferometer imaging system for thermospheric temperature and wind measurements.

Doppler-broadened Fabry-Perot fringes of weak (<1-kR) auroral [OI] 5577- and 6300-A emissions have been detected in real time (1/60 sec) with the aid of a low-light level image-orthicon TV camera coupled to a two stage image intensifier. The imaging scheme permits static-mode operation of a Fabry-Perot interferometer. For maximum use of all the information contained in every TV frame, each circular fringe may be sectioned into several annuli, and the corresponding annuli from all rings are summed to yield an intensity value. This procedure for deriving a fringe profile requires a video digitizer coupled to a digital processing system and should provide fast real-time (1/60 sec) measurements of E- and F-region temperatures and winds. A more restricted analysis of the TV images involves analog display of the intensity distribution along a few raster lines. Since only a minute fraction of the total signal is thus utilized to derive a fringe profile, image integration on the TV camera is necessary to assure high SNR of the profile. With [OI] 5577-A intensity of ~750 R, visually prominent TV images of the Fabry-Perot fringes were recorded in 0.5 sec, and the temperature of the emitting region was determined from ~2% of the total information in the TV image.