The hemagglutinating activity of Pseudomonas aeruginosa strains isolated from humans and animals.

The presence and type of adhesins occurring in Pseudomonas aeruginosa strains were determined by hemagglutination test with a 3% suspension of normal and trypsin-treated human group A erythrocytes, with or without the addition of sugar inhibitors (D-mannose, D-glucose, L-fucose, D-galactose, D-fructose, lactose, N-acetylneuraminic acid, N-acetylglucosamine and N-acetylgalactosamine). This study showed that a low percentage of Pseudomonas aeruginosa strains caused the agglutination of normal erythrocytes. Trypsin treatment of erythrocytes did not affect the hemagglutinating properties, indicating that hemagglutination was not dependent upon a trypsin-sensitive protein on the erythrocytes surface. Most of the studied strains agglutinated RBCs at 37 degrees C. A great variability in the inhibiting activity on studied strains was observed among the carbohydrates tested. These results demonstrated the predominant role of N-acetylglucosamine, N-acetylgalactosamine and N-acetylneuraminic acid for Pseudomonas aeruginosa adhesion to RBCs.

[Clinical manifestations of extrinsic and intrinsic sinus node dysfunction (functional and organic factors)]. / Zespól niewydolnosci wezla zatokowego (postac czynnosciowa i organiczna). Obraz kliniczny.

UNLABELLED: We analysed the clinical data of 206 patients (pts) with sick sinus syndrome (group S). All the population was divided into two group; pts with normal (104 pts, group S1) and abnormal (102 pts, group S2) "intrinsic" properties of sinus node (SN). Intrinsic properties was defined as normal if intrinsic heart rate was normal and corrected sinus node recovery time after pharmacological heart denervation (propranolol 0.2 mg/kg body weight, atropine 0.04 mg/kg body weight) was no longer than 422 ms. In whole group S, in 131 pts we found organic heart disease (coronary heart disease, n = 102, hypertensive disease, n = 12, mitral valve prolapse, n = 10, history of myocarditis or mild mitral or aortic valve stenosis, n = 7). In groups S1 and S2 coronary heart disease was found in 31 and 71 pts, hypertensive disease in 6 and 6 pts respectively. All the patients with mitral valve prolapse were found in group S1. In 75 pts sinus node disease (SSS) was the only pathological finding, they were divided into group IS1 (n = 51) and IS2 (n = 24) according to the intrinsic properties of the SN. CONCLUSION: 1. In clinical practice sinus node dysfunction seems to be more frequent in men than in women, 2. In the large group of patients with sick sinus syndrome we analysed in this paper in about 36% we did not find any other diseases of the heart.(ABSTRACT TRUNCATED AT 250 WORDS)

[QT interval and the autonomic nervous system in coronary disease and sick sinus syndrome]. / Odstep QT elektrokardiogramu a uklad wegetatywny w chorobie wiencowej i niewydolnosci wezla zatokowego.

The autonomous nervous system influence on sinus node (SN) in both physiological and pathological states is the most important factor determining its function. There are only few publications about autonomous nervous system action on electrophysiological properties of the human heart (ventricles), specially in patients with different types of sick sinus syndrome (SSS). Electrocardiograms of 113 patients (pts) with coronary heart disease, suspected for SSS, recorded during transesophageal atrial pacing before and after pharmacological denervation of the heart, were analysed. For heart denervation propranolol (0.2 mg/kg body weights) and atropine (0.04 mg/kg body weights) intravenously were used. We analysed sinus cycle length (P-P), QT interval and QTc (QT interval corrected according to Bazett's formula) during both sinus and paced rhythm (100 bpm), before and after denervation of the heart. During clinical observations (including Holter monitoring) and after electrophysiological testing we excluded SSS in 28 pts (group A) and confirmed diagnosis of SSS in 85 pts (group B). Group B was divided in two parts; pts with normal (group B1) and abnormal (group B2) intrinsic properties of the SN. Group B1 included 23 pts (15 men and 8 woman from 36 to 74, mean 53 +/- 9) with normal sinus cycle length after denervation (according to Joses's formula) and maximal, corrected sinus node recovery time after denervation less than 422 ms. Group B2 included remaining 62 pts (26 men and 36 woman from 36 to 80, mean 59 +/- 9). In groups A and B1 denervation of the heart shortens P--P, QT, QT100 and QTc100 significantly. Only QTc in A and B1 and all parameters in group B2 didn't change significantly.(ABSTRACT TRUNCATED AT 250 WORDS)

[Effect of selective and non-selective adrenergic beta receptor blockaders on selected electrophysiologic properties of the human heart]. / Wplyw selektywnej i nieselektywnej blokady receptorów adrenergicznych beta na wybrane wlasciwosci elektrofizjologiczne serca czlowieka.

Beta-2 adrenergic receptors were found in a human heart and their role in regulation of inotropic properties during last year was proved. The aim of our study was comparison of effects of selective (metoprolol) and nonselective (propranolol) beta-receptors blockade on cardiac electrophysiological properties. The study was carried out in 20 patients in the majority without organic heart injury and clinical symptoms of the sinus node dysfunction as well as atroioventricular conduction disorders. Method of the transoesophageal left atrial stimulation was utilized. Examinations were performed after drugs administrations in the same persons, during following days. Propranolol and metoprolol were intravenously administrated in a dose of 0.2 mg/kg b.w. Both drugs statistically significant lengthened sinus rhythm cycle time, sinus node recovery time, sinus node and a-v node effective refraction and lowered Wenckebach's point. They did not significantly effect on sinoatrial conduction and the atrial effective refraction. There were no significant differences between examined beta-blockers. Obtained results allowed us to conclude that: 1) eletrophysiologic properties of propranolol and metoprolol are similar, 2) it seems that in physiological conditions the effects of the adrenergic nervous system on electrophysiologic properties of sinus node, atrio-ventricular node and atrium is mainly realized by beta-1 adrenergic receptors.