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Cloning and expression in Escherichia coli of the Azospirillum brasilense Sp7 gene encoding ampicillin resistance.
Verreth, C; Cammue, B; Swinnen, P; Crombez, D; Michielsen, A; Michiels, K; Van Gool, A; Vanderleyden, J.
Appl Environ Microbiol ; 55(8): 2056-60, 1989 Aug.
Article in English | MEDLINE | ID: mdl-2675764

ABSTRACT

The Azospirillum brasilense ATCC 29145 gene coding for beta-lactamase was cloned in Escherichia coli. The gene was expressed in E. coli from its own promoter as a 30-kilodalton protein, conferring resistance to high levels of beta-lactam antibiotics. The DNA sequence containing the beta-lactamase gene was found to be highly amplified in the Azospirillum genome, scattered in the chromosomal as well as in the plasmidic DNA.


Subject(s)
Ampicillin Resistance/genetics , Escherichia coli/genetics , Gene Expression Regulation , Gram-Negative Bacteria/genetics , beta-Lactamases/genetics , Blotting, Southern , Centrifugation, Density Gradient , Cloning, Molecular , DNA, Bacterial/genetics , Escherichia coli/drug effects , Escherichia coli/enzymology , Genes, Bacterial , Gram-Negative Bacteria/drug effects , Gram-Negative Bacteria/enzymology , Nucleic Acid Hybridization , Restriction Mapping , beta-Lactamases/biosynthesis
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