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1.
Pure Appl Chem ; 70(2): 263-6, 1998 Feb.
Article in English | MEDLINE | ID: mdl-11542721

ABSTRACT

A research program has applied the tools of synthetic organic chemistry to systematically modify the structure of DNA and RNA oligonucleotides to learn more about the chemical principles underlying their ability to store and transmit genetic information. Oligonucleotides (as opposed to nucleosides) have long been overlooked by synthetic organic chemists as targets for structural modification. Synthetic chemistry has now yielded oligonucleotides with 12 replicatable letters, modified backbones, and new insight into why Nature chose the oligonucleotide structures that she did.


Subject(s)
DNA/chemistry , Molecular Biology/trends , Nucleic Acids/chemistry , Oligonucleotides/chemical synthesis , Catalysis , Codon , Molecular Structure , Nucleic Acids/chemical synthesis , Oligonucleotides/chemistry , Phosphates/chemistry , Sulfones/chemistry
2.
Biochemistry ; 32(39): 10489-96, 1993 Oct 05.
Article in English | MEDLINE | ID: mdl-7691174

ABSTRACT

The ability of various polymerases to catalyze the template-directed formation of a base pair between isoguanine (iso-G) and isocytosine (iso-C) in duplex oligonucleotides has been investigated. A new procedure was developed for preparing derivatives of deoxyisoguanosine suitable for incorporation into DNA using an automated DNA synthesizer. T7 RNA polymerase, AMV reverse transcriptase, and the Klenow fragment of DNA polymerase all incorporated iso-G opposite iso-C in a template. T4 DNA polymerase did not. Several polymerases also incorporated iso-G opposite T, presumably through pairing with a minor tautomeric form of iso-G complementary to T. In a template, iso-G directs the incorporation of both iso-C and T when Klenow fragment is the catalyst and only U when T7 RNA polymerase is the catalyst. Further, derivatives of iso-C were found to undergo significant amounts of deamination under alkaline conditions used for base deprotection after automated oligonucleotide synthesis. Both the deamination reaction of iso-C and the ambivalent tautomeric forms of iso-G make it unlikely that the (iso-C).(iso-G) base pair was a part of information storage molecules also containing the A.T and G.C base pairs found in primitive forms of life that emerged on planet earth several billion years ago. Nevertheless, the extra letters in the genetic alphabet can serve useful roles in a contemporary laboratory setting.


Subject(s)
Base Composition , Cytidine/metabolism , DNA Polymerase I/metabolism , DNA-Directed RNA Polymerases/metabolism , Guanosine/metabolism , RNA-Directed DNA Polymerase/metabolism , Adenosine , Base Sequence , Molecular Sequence Data , Substrate Specificity , Viral Proteins
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