ABSTRACT
Boron neutron capture therapy (BNCT) can become an instrument for patients with malignant neoplasms of the rectum and colon. Here we evaluate the effectiveness of BNCT performed at the accelerator based epithermal neutron source at G. I. Budker Institute of Nuclear Physics, Siberian Division of Russian Academy of Sciences, in relation to subcutaneous xenografts of human colon adenocarcinoma SW-620 in SCID mice. Utilization of BNCT with boronоphenylalanine (BPA) and sodium borocaptate (BSH), which were injected intravenously into the retroorbital sinus, resulted in a significant decrease in tumor volumes compared to the control group (no radiation).
Subject(s)
Adenocarcinoma , Boron Neutron Capture Therapy , Brain Neoplasms , Colorectal Neoplasms , Adenocarcinoma/radiotherapy , Animals , Boron Neutron Capture Therapy/methods , Colorectal Neoplasms/radiotherapy , Heterografts , Humans , Mice , Mice, SCID , Sulfhydryl CompoundsABSTRACT
Objective: To investigate the impact of a neutron beam formed with the accelerator-based epithermal neutron source designed at the G.I. Budker Institute of Nuclear Physics (INP) on the viability of human and animal tumor cells cultured in the presence of boron-10 isotope. Material and Methods: Human U251 and T98G glioma cells and Chinese hamster CHO-K1 and V-79 cells were incubated at various concentrations in the culture medium containing 10B-enriched L-boronophenylalanine. The cells were irradiated with a neuron beam using the accelerator-based epithermal neuron source. A clonogenic assay was used to evaluate the viability of the irradiated cells. The absorbed doses obtained from elastic scattering of fast neutrons by substance nuclei and the doses obtained from boron neutron capture were calculated using the NMS code. The absorbed doses of gamma-radiation were measured with a mixed radiation dosimeter. Results: The viability of boron-containing and intact human U251 and T98G cell lines and Chinese hamster CHO-K1 and V-79 cells was analyzed after neutron beam radiation. Irradiation of all four cell lines were cultured in the presence of 10B was shown to reduce their colony-forming capacity compared with the control. Elevated boron levels in the culture medium resulted in a significant decrease in the proportion of survived cells. Radiation had the most pronounced impact on the proliferative capacity of the human U251 glioma cell lines. Conclusion: The cultures of human tumor cells and mammalian cells demonstrated that the neutron beam formed with the accelerator-based epithermal neutron source designed at the INP, was effective in reducing the viability of tumor cells in the presence of 10B.
Subject(s)
Boron Neutron Capture Therapy/methods , Boron/pharmacology , Isotopes/pharmacology , Animals , CHO Cells/radiation effects , Cell Line, Tumor/radiation effects , Cell Survival , Cricetulus , Dose-Response Relationship, Radiation , Glioma/radiotherapy , HumansABSTRACT
Half-body irradiation at a single dose of 400-800 cGy was delivered to patients with disseminated fast-growing types of lymphomas and large aggregates of tumors and vital dysfunction of the body (the compression syndrome). Active detoxification therapy including heparinization, hemodilution with enforced diuresis and hemosorption was performed to prevent the syndrome of acute tumor lysis and postradiation complications. This treatment modality resulted in the elimination of clinical manifestations of the compression syndrome, normalization of the body homeostasis, a considerable reduction of the volume of tumor aggregates on a short-term basis, entering the patients into a curable state; the 5-year follow-up results were evaluated.
