Diameter and Taper Variability of Single-file Instrumentation Systems and Their Corresponding Gutta-percha Cones.

INTRODUCTION: Manufacturers offer single-file instrumentation systems with matching gutta-percha (GP) cones to simplify root canal preparation and obturation. The purpose of this study was to determine whether file diameters and tapers match with corresponding cone diameters and tapers (precision) as well as industry standards (accuracy). METHODS: Twenty files and corresponding GP cones from each size of F360 (#25, #35, #45, #55 with .04 taper) and Reciproc (#25, #40, #50 with variable tapers) instruments were examined by using optical microscopy (×32) to determine their diameter and taper. Precision was evaluated by using one-way analysis of variance (α = 0.05) with Scheffé post hoc tests and t tests with Bonferroni correction. Accuracy was calculated by subtracting the nominal values from the measured values of all files and GP cones, and mean diameter and taper differences were compared by using one-way analysis of variance (α = 0.05) and Scheffé post hoc test for pairwise comparison. RESULTS: For F360, the majority of file and cone diameters were within the tolerance levels, but most of the file diameters were significantly larger than GP cone diameters (P < .05), but the majority of all measured values were within the tolerance levels. For Reciproc, file and cone diameters at D1 and D3 mostly approached the nominal values. At the coronal end, file diameters #25 and #50 were significantly smaller than cone diameters (P < .05). For both instrumentation systems, almost all file and cone tapers matched with the preset tolerance ranges. For Reciproc, significant differences between file and GP cone demonstrated either smaller cone or smaller file diameters and tapers, depending on the size. Most of the measured values were within the acceptable range, but diameters at the coronal end exhibited the highest percent difference from the nominal values. CONCLUSIONS: Despite the call for standardization, variability in diameter and taper dimensions between single-file instrumentation systems and their corresponding GP cones can be expected.

Comparison of the antibacterial efficacy of sonic- and two ultrasonic-activated irrigation techniques in reducing intracanal Enterococcus faecalis populations.

OBJECTIVE: This in-vitro study evaluated the antibacterial efficacy of sonic and ultrasonic irrigation with either intermittent or continuous flush in root canals infected with Enterococcus faecalis. METHOD AND MATERIALS: Seventy single-rooted, extracted teeth with straight root canals were prepared with FlexMaster NiTi-instruments, sterilized, inoculated with E faecalis and incubated for 72 hours. Sixty roots were randomly divided into four experimental groups as well as into infection and sterile controls (n = 10 each). The remaining teeth were used for SEM analysis of smear layer removal (n = 5) and infection with E faecalis (n = 5). Irrigation in the experimental groups was performed with 1% NaOCl and syringe irrigation, sonic activation of the irrigant with EndoActivator, ultrasonic activation with intermittent flush, or ultrasonic activation with continuous flush. Microbial sampling was performed by collecting dentin shavings from the root canal walls. Colony-forming units were counted and statistical analysis was performed using nonparametric rank-based ANOVA-type tests for longitudinal data (P < .05). RESULTS: A significant reduction of E faecalis of 3 log10 steps compared to the infection control was achieved by all irrigation techniques (P < .0001). There were no significant differences in antibacterial efficacy between syringe irrigation, sonically, or ultrasonically activated irrigation (P > .45). CONCLUSION: Activation of 1% NaOCl with either sonic or ultrasonic devices did not increase the antimicrobial efficacy against E faecalis compared to conventional syringe irrigation in straight root canals.

Blechschmidt Collection: Revisiting specimens from a historical collection of serially sectioned human embryos and fetuses using modern imaging techniques.

Along with the Carnegie Collection in the United States and the Kyoto Collection in Japan, the Blechschmidt Collection (Georg-August-University of Göttingen, Germany) is a major historical human embryo and fetus collection. These collections are of enormous value to human embryology; however, due to the nature of the historical histological specimens, some stains are fading in color, and some glass slides are deteriorating over time. To protect these specimens against such degradation and ensure their future usefulness, we tried to apply modern image scanning and computational reconstruction. Samples of histological specimens of the Blechschmidt Collection were digitized into images using commercial flatbed scanners with a resolution of 4800 pixels per inch. Two specimens were reconstructed into three-dimensional (3D) images by using modern techniques to vertically stack two-dimensional images of the slices into 3D blocks. The larger specimen of crown-rump length (CRL) 64.0 mm, a series of very large histological sections in human embryology, was reconstructed clearly, with its central nervous system segmented before stacking. The smaller specimen of CRL 17.5 mm was also reconstructed into 3D images. The outer surface of the embryo was intact, and its development was classified according to the widely used Carnegie stages (CSs). The CS of the specimen was identified as the later half of CS 20. The invaluable Blechschmidt Collection can be revisited for further research with modern techniques such as digital image scanning and computational 3D reconstruction.

An Early Chick Embryo Culture Device for Extended Continuous Observation.

Appropriate mechanical tension of the vitelline membrane as the culture substrate for the early chick embryo is frequently reported to be required for successful in vitro development. Here we describe a modified device, made of anodized aluminum, for in vitro culture which we used for studies of left-right symmetry breaking with emphasis on morphology and gene expression as readouts. The technique allows for easy, high-throughput tissue handling and provides a suitable tension in a stable and easily reproducible manner proven to be suitable for correct molecular left-right patterning and heart looping after long-term culture.

Effect of fiber insertion depth on antibacterial efficacy of photodynamic therapy against Enterococcus faecalis in rootcanals.

OBJECTIVES: This in vitro study evaluated the effect of fiber insertion depth on antimicrobial efficacy of antimicrobial photodynamic therapy (aPDT) using a photosensitizer (PS; toluidine blue) and a red light-emitting diode (LED) in root canals infected with Enterococcus faecalis. MATERIALS AND METHODS: Single-rooted extracted teeth were prepared with nickel-titanium-instruments, sterilized, contaminated with E. faecalis, and incubated for 72 h. Roots were randomly divided into four experimental groups: PS only, LED only, aPDT with LED in the apical third, aPDT with LED in the coronal third, as well as into infection and sterile controls (each n = 10). Samples were taken by collecting standardized dentine shavings from the root canal walls. After serial dilution and culturing on blood agar, colony-forming units (CFU) were counted. RESULTS: Both aPDT groups showed a CFU reduction of 1-2 log10 steps compared with the infection control, whereas the effect of fiber insertion depth was negligible (<0.5 log10 steps). CFU reduction of approximately 0.5 log10 steps for PS alone was detected compared with the infection control, but PS alone was less effective than both aPDT groups. No antibacterial effect was detected for LED alone. CONCLUSIONS: aPDT reduced E. faecalis within the root canal, whereas fiber insertion depth had a negligible influence on antimicrobial effectiveness of aPDT. CLINICAL RELEVANCE: The insertion depth of the light-emitting diode may not influence the antibacterial efficacy of photodynamic therapy against E. faecalis in straight root canals.