Land-use induced soil carbon stabilization at the expense of rock derived nutrients: insights from pristine Andean soils.

Soils contain significantly more carbon than the atmosphere, hence we should understand how best to stabilize it. Unfortunately, the role of human interventions on soil organic carbon (SOC) persistence in the Anthropocene remains vague, lacking adequate sites that allow unbiased direct comparisons of pristine and human influenced soils. Here we present data from a unique study system in the High Andes that guarantees pristineness of the reference sites by physical inaccessibility through vertical cliffs. By comparing the isotopic signatures of SOC, mineral related carbon stabilization, and soil nutrient status across grazed versus pristine soils, we provide counterintuitive evidence that thousands of years of pastoralism increased soil C persistence. Mineral associated organic carbon (MAOC) was significantly higher in pastures. Land use increased poorly crystalline minerals (PCM's), of which aluminum correlated best with MAOC. On the other hand, human's acceleration of weathering led to acidification and higher losses of cations. This highlights a dilemma of lower soil quality but higher persistence of SOC due to millennia of pastoralism. The dynamics of soil genesis in the Anthropocene needs better understanding, but if human-induced weathering proves generally to promote soil carbon persistence it will need to be included in climate-soil feedback projections.

More Than Words? How Highlighting Target Populations Affects Public Opinion about the Medicaid Program.

CONTEXT: The Medicaid program provides health insurance coverage to a diverse set of demographics. We know little about how the policy community describes these populations (e.g., on Medicaid-related websites or in public opinion polls and policy writings) or whether and how these descriptions may affect perceptions of the program, its beneficiaries, and potential policy changes. METHODS: To investigate this issue, we developed and fielded a nationally representative survey of 2,680 Americans that included an experiment for priming respondents by highlighting different combinations of target populations of the Medicaid program as found in the Medicaid policy discourse. FINDINGS: Overall, we find that Americans view Medicaid and its beneficiaries rather favorably. However, there are marked differences based on partisanship and racial animosity. Emphasizing citizenship and residency requirements at times improved these perceptions. CONCLUSIONS: Racial perceptions and partisanship are important correlates in Americans' views about Medicaid and its beneficiaries. However, perceptions are not immutable. In general, the policy community should shift toward using more comprehensive descriptions of the Medicaid population that go beyond the focus on low income and that include citizenship and residency requirements. Future research should expand this work by studying descriptions in the broader public discourse.

Vaccinating across the aisle: using co-partisan source cues to encourage COVID-19 vaccine uptake in the ideological right.

Overcoming the COVID-19 pandemic in the United States will require most Americans to vaccinate against the disease. However, considerable research suggests that a significant proportion of Americans intend to forego vaccination, putting pandemic recovery at risk. Republicans are one of the largest groups of COVID-19 vaccine hesitant individuals. Therefore, identifying strategies to reduce vaccine hesitancy within this group is vital to ending the pandemic. In this study, we investigate the effectiveness of messages from co-partisan sources in reducing vaccine hesitancy. In a large (N = 3000) and demographically representative survey, we find that exposing "Middle-of-the-Road" partisans to pro-vaccine messages from co-partisan source cues reduces vaccine hesitancy. However, for those who identify as "Strong" or "Weak" partisans, we find no statistically significant differences in vaccination intentions when exposed to pro-vaccine messages from co-partisan sources. We conclude by discussing how our findings are helpful for vaccine communication efforts.

Plastid genomes reveal evolutionary shifts in elevational range and flowering time of Osmanthus (Oleaceae).

Species of Osmanthus are economically important ornamental trees, yet information regarding their plastid genomes (plastomes) have rarely been reported, thus hindering taxonomic and evolutionary studies of this small but enigmatic genus. Here, we performed comparative genomics and evolutionary analyses on plastomes of 16 of the 28 currently accepted species, with 11 plastomes newly sequenced. Phylogenetic studies identified four main lineages within the genus that are here designated the: "Caucasian Osmanthus" (corresponding to O. decorus), "Siphosmanthus" (corresponding to O. sect. Siphosmanthus), "O. serrulatus + O. yunnanensis," and "Core Osmanthus: (corresponding to O. sect. Osmanthus + O. sect. Linocieroides). Molecular clock analysis suggested that Osmanthus split from its sister clade c. 15.83 Ma. The estimated crown ages of the lineages were the following: genus Osmanthus at 12.66 Ma; "Siphosmanthus" clade at 5.85 Ma; "O. serrulatus + O. yunnanensis" at 4.89 Ma; and "Core Osmanthus: clade at 6.2 Ma. Ancestral state reconstructions and trait mapping showed that ancestors of Osmanthus were spring flowering and originated at lower elevations. Phylogenetic principal component analysis clearly distinguished spring-flowering species from autumn-flowering species, suggesting that flowering time differentiation is related to the difference in ecological niches. Nucleotide substitution rates of 80 common genes showed slow evolutionary pace and low nucleotide variations, all genes being subjected to purifying selection.

Comparative analysis of the complete plastid genomes in Prunus subgenus Cerasus (Rosaceae): Molecular structures and phylogenetic relationships.

Prunus subgenus Cerasus (cherry) is an economically important group that distributed in temperate regions of the northern hemisphere. However, shared interspecific morphological traits and variability across taxa of Cerasus are among the impediments to taxonomic efforts to correctly delimit taxa. This is further complicated by a lack of genetic information on these taxa, with no focused genomic or phylogenetic studies being done on Cerasus. In this study, we conducted comparative analysis on the complete plastid genomes (plastomes) of 20 Cerasus species to gain a greater understanding of the attributes of the plastome of these taxa while helping resolve their phylogenetic placement in Prunus sensu lato and interspecific relationships within the subgenus. Our results displayed that (1) the plastomes of the 20 Cerasus species studied exhibited a typical quadripartite structure with conversed genome arrangement, structure, and moderate divergence. (2) The average size of complete plastomes for the Cerasus taxa studied was 157,861 bp, ranging from 157,458 to 158,024 bp. A total of 134 genes were annotated, including 86 protein-coding genes, 40 tRNAs, and 8 rRNAs across all species. In simple sequence repeat analysis, we found Cerasus had a comparable number of dispersed and tandem repeats to those identified in other angiosperm taxa, with only P. pseudocerasus found to contain trinucleotide repeats. Nucleotide diversity analysis revealed that the trnG-GCC gene and rpl32-trnL region had the highest Pi value showing potential as phylogenetic markers. (3) Two phylogenetic trees of the plastomes verified the monophyletic relationship of Cerasus and provided a more resolved species-level phylogeny. Our study provides detailed plastome information for exploring the phylogeny of subg. Cerasus taxa. We identified various types of repeats and nucleotide diversity hotspots, which can be a reference for species identification and reconstruction of phylogenetic relationships.

Past experiences with surprise medical bills drive issue knowledge, concern and attitudes toward federal policy intervention.

Scholars and journalists have devoted considerable attention to understanding the circumstances in which Americans receive surprise medical bills. Previous research on this issue has focused on the scope of the problem, including the conditions that are most likely to lead to surprise bills. However, the existing literature has almost exclusively relied on claims data, limiting our understanding of consumer experiences and attitudes toward policy changes to address surprise billing. Using a survey administered to a nationally representative sample of 4998 Americans, we analyze consumer experiences with surprise billing, knowledge of the issue, how concerned Americans are about receiving surprise bills and how past experiences influence policy preferences toward federal action on surprise billing. Our analysis demonstrates that knowledge and concern about surprise billing are the highest among the educated and those who have previously received a surprise bill. These factors also predict support for federal policy action, with high levels of support for federal policy action across the population, including among both liberals and conservatives. However, more detailed federal policy proposals receive significantly less support among Americans, suggesting that stand-alone policy action may not be viable. Our results show bipartisan support among American consumers for federal action on surprise billing in the abstract but no consistent views on specific policy proposals.

Evolutionary patterns of nucleotide substitution rates in plastid genomes of Quercus.

Molecular evolution, including nucleotide substitutions, plays an important role in understanding the dynamics and mechanisms of species evolution. Here, we sequenced whole plastid genomes (plastomes) of Quercus fabri, Quercus semecarpifolia, Quercus engleriana, and Quercus phellos and compared them with 14 other Quercus plastomes to explore their evolutionary relationships using 67 shared protein-coding sequences. While many previously identified evolutionary relationships were found, our findings do not support previous research which retrieve Quercus subg. Cerris sect. Ilex as a monophyletic group, with sect. Ilex found to be polyphyletic and composed of three strongly supported lineages inserted between sections Cerris and Cyclobalanposis. Compared with gymnosperms, Quercus plastomes showed higher evolutionary rates (Dn/Ds = 0.3793). Most protein-coding genes experienced relaxed purifying selection, and the high Dn value (0.1927) indicated that gene functions adjusted to environmental changes effectively. Our findings suggest that gene interval regions play an important role in Quercus evolution. We detected greater variation in the intergenic regions (trnH-psbA, trnK_UUU-rps16, trnfM_CAU-rps14, trnS_GCU-trnG_GCC, and atpF-atpH), intron losses (petB and petD), and pseudogene loss and degradation (ycf15). Additionally, the loss of some genes suggested the existence of gene exchanges between plastid and nuclear genomes, which affects the evolutionary rate of the former. However, the connective mechanism between these two genomes is still unclear.

COVID-19 and Motivated Reasoning: The Influence of Knowledge on COVID-Related Policy and Health Behavior.

Objective: The spread of misinformation about COVID-19 severely influences the governments' ability to address the COVID-19 pandemic. This study explores the predictors of accurate beliefs about COVID-19 and its influence on COVID-related policy and behavior. Methods: Data from an original survey collected by Lucid in July 2020 are used. Ordinary Least Squares regression (OLS) is used to predict accurate beliefs about COVID-19. Ordered logistic regression models are estimated to examine the relationship between COVID-19 knowledge, policy preferences, and health behavior intentions. Results: Ideology and education were found to have a positive effect on knowledge about COVID-19. Moreover, low levels of knowledge about COVID-19 were found to reduce support for mandatory vaccination policy and willingness to get a coronavirus vaccine when available. Conclusion: These findings will help policymakers develop communication strategies for the public on the coronavirus vaccination.

Lingering Legacies: Public Attitudes about Medicaid Beneficiaries and Work Requirements.

CONTEXT: This article provides a detailed picture of the mindset of Americans about Medicaid work requirements and the important roles that perception of deservingness and racial bias play in public attitudes. METHODS: The authors conducted a large original survey to investigate public attitudes toward work requirements. They analyzed the predictors of overall support for work requirements, correlates of who should be exempt from them, and attitudes toward work supports that make compliance with work requirements easier. FINDINGS: The authors found that public opinion is split relatively evenly when it comes to Medicaid work requirements in the abstract. When Americans are confronted with the complexities of the issue, important nuances emerge. The authors also found consistent evidence that support for work requirements is higher among conservatives, those who see Medicaid as a short-term program, and racially resentful non-Hispanic whites. They show that groups that have historically been framed as deserving see high levels of support for their exemption (e.g., the disabled and senior citizens). Finally, the authors found that Americans are supportive of policies that provide individuals with help when transitioning into the workforce. CONCLUSIONS: Americans' views of Medicaid and the populations it serves are complex and continue to be influenced by perceptions of deservingness and race.

A phylogeny of species near Agrostis supporting the recognition of two new genera, Agrostula and Alpagrostis (Poaceae, Pooideae, Agrostidinae) from Europe.

Based on a molecular DNA phylogeny of three plastid (rpl32-trnK, rps16 intron, and rps16-trnK) and nuclear ITS regions investigating 32 species of Agrostidinae, we describe two new genera, Agrostula gen. nov. with a single species and Alpagrostis gen. nov. with four species; provide support for five species in a monophyletic Podagrostis; and include a small sample of 12 species of a monophyletic Agrostis s.s. (including the type and most species of Neoschischkinia), that separates into two clades corresponding to A. subg. Agrostis and A. subg. Vilfa. Agrostula differs from Agrostis in having leaf blades with pillars of sclerenchyma which are continuous between the adaxial and abaxial surface of the blades, dorsally rounded glumes with blunt to truncate and erose to denticulate apices, florets ½ the length of the glumes, lemmas equally wide as long, widest at (or near) apex, apices broadly truncate, irregularly 5 to 7 denticulate to erose, awnless, anthers longer than the lemmas, and rugose-papillose caryopses. Alpagrostis differs from Agrostis in having geniculate basally inserted awns and truncate lemma apices with lateral veins prolonged from the apex in (2)4 setae. The following eight new combinations are made: Agrostula truncatula, Agrostula truncatula subsp. durieui, Alpagrostis alpina, Alpagrostis alpina var. flavescens, Alpagrostis barceloi, Alpagrostis setacea, Alpagrostis setacea var. flava, and Alpagrostis schleicheri. In addition, we provide a key separating Agrostula and Alpagrostis from Agrostis s.s. and other genera previously considered as synonyms of Agrostis; lectotypify Agrostis alpina Scop., A. schleicheri Jord. & Verl., A. truncatula Parl., and A. truncatula var. durieui Henriq.; and neotypify A. setacea Curtis.

ResumenSobre la base de una filogenia molecular de ADN de tres regiones plastidiales (rpl32-trnK, rps16 intrón y rps16-trnK) e ITS nuclear de 32 especies de Agrostidinae, describimos dos nuevos géneros, Agrostula gen. nov. con una sola especie, y Alpagrostis gen. nov. con cuatro especies; mostramos el apoyo para las cinco especies dentro de Podagrostis monofilético; e incluimos una pequeña muestra de 12 especies de Agrostis s.s (que incluye el tipo y la mayoría de las especies de Neoschischkinia), este último dividido en dos subclados que corresponden a A. subg. Agrostis y A. subg. Vilfa. Agrostula se diferencia de otras especies de Agrostis por tener láminas foliares con haces de esclerénquima continuos entre las superficies adaxial y abaxial de los limbos, glumas de dorso redondeado y ápice embotado a truncado y eroso a denticulado, antecios de ½ de la longitud de las glumas, lemas tan anchas como largas, lo más ancho en o cerca del ápice, ápices anchamente truncados, irregularmente 5 a 7 denticulados o erosos, sin arista, anteras más largas que los lemas y cariopsis rugosa-papilosa. Alpagrostis se diferencia de otras especies de Agrostis por tener aristas geniculadas insertas basalmente y ápices de lema truncados con venas laterales que se prolongan en (2)4 arístulas apicales. Presentamos las siguientes ocho nuevas combinaciones: Agrostula truncatula, Agrostula truncatula subsp. durieui, Alpagrostis alpina, Alpagrostis alpina var. flavescens, Alpagrostis barceloi, Alpagrostis setacea, Alpagrostis setacea var. flava y Alpagrostis schleicheri. Además, proporcionamos una clave que separa Agrostula y Alpagrostis de Agrostis s.s. y otros géneros previamente considerados como sinónimos de Agrostis, lectotipificamos Agrostis alpina Scop., A. schleicheri Jord. & Verl., A. truncatula Parl. y A. truncatula var. durieui Henriq. y neotipificamos A. setacea Curtis.

Re-instatement of Sorbus harrowiana (Rosaceae), based on morphometric analysis.

Sorbus harrowiana (≡Pyrus harrowiana), previously considered a synonym of Sorbus insignis (≡Pyrus insignis) in the Flora of China, is re-instated here and shown to be distinct from S. insignis, based on morphometric analysis, coupled with herbarium and field investigation. We also present for the first-time full descriptions, distributional records and notes for S. harrowiana and S. insignis.

New records and key to Poa (Pooideae, Poaceae) from the Flora of Southern Africa region and notes on taxa including a diclinous breeding system in Poa binata.

Four species of Poa L. are newly reported for sub-Saharan Africa and southern Africa, Poa compressa L., P. iconia Azn., P. infirma Kunth and P. nemoralis L. This is the first report of P. iconia from Africa. Vouchers at PRE of P. bulbosa L. all belong to var. vivipara Koeler, those of P. iconia belong to var. iconia and the one of P. trivialis L. belongs to var. trivialis. Two subspecies are recognised in P. pratensis L.: subsp. irrigata (Lindm.) H.Lindb. and subsp. pratensis. We also designate a lectotype for P. iconia and second-step lectotype for P. leptoclada Hochst. ex A.Rich. and report the first recording of a diclinous breeding system in P. binata Nees. Our account updates the treatment in Identification Guide to Southern African Grasses (Fish et al. 2015) including a key to the taxa and notes on infrageneric taxonomy, DNA subtypes, ecology, chromosome numbers and breeding systems.

Festuca drakensbergensis (Poaceae): A common new species in the F. caprina complex from the Drakensberg Mountain Centre of Floristic Endemism, southern Africa, with key and notes on taxa in the complex including the overlooked F. exaristata.

We present taxonomic notes on the Festuca caprina complex from southern Africa that includes description and illustration of the new species F. drakensbergensis from the Drakensberg Mountain Centre of Floristic Endemism of South Africa and Lesotho. Festuca drakensbergensis can be differentiated from F. caprina s.l. by forming lax short tufts with extravaginally-branching tillers and lateral-tending cataphyllous shoots or rhizomes present, basal foliage reaching < ½ the length of the culms, with generally shorter leaves and shorter anthers, 0.8-1.6(-1.8) mm long. The species also differs from the overlooked species F. exaristata - currently known from two collections from Lesotho - by its fibrous basal sheaths, usually sharp, keel-like leaf blade midrib, drooping panicle with lightly to densely scabrous pendent panicle branches, longer lemmas, 4.5-5.8 mm long, with awns usually present, 0.5-3 mm long, ovary apices sparsely to densely hairy and anthers 0.8-1.6(-1.8) mm long. Taxonomic notes on the different taxa of the F. caprina complex in southern Africa are also provided, including images, key, and lectotypification of F. caprina var. curvula. This research adds a further two endemic species (F. drakensbergensis and F. exaristata) and two endemic varieties (F. caprina var. irrasa and F. caprina var. macra) to the Drakensberg Mountain Centre of Floristic Endemism.

Agrostis and Podagrostis (Agrostidinae, Poaceae) from páramos of Boyacá, Colombia: synoptic taxonomy including a key to Colombian species.

We present taxonomic notes, including updated species descriptions and images, for the nine species of Agrostis and one species of Podagrostis found in páramos of Departamento Boyacá, Colombia (A. boyacensis, A. breviculmis, A. capillaris, A. foliata, A. cf. imberbis, A. mertensii, A. perennans s.l., A. stolonifera, A. tolucensis, Podagrostis trichodes). Agrostis cf. imberbis, previously known from austral South America, is newly recorded for Colombia, A. capillaris is a new regional record for Boyacá, and the name Agrostis stuebelii is lectotypified. We include keys in English and Spanish to distinguish the 15 species of Agrostis and two species of Podagrostis that are cited as occurring in Colombia.

ResumenSe presentan notas taxonómicas, además de descripciones e imágenes actualizadas, para las nueve especies de Agrostis y una especie de Podagrostis encontradas en los páramos del departamento de Boyacá, Colombia (A. boyacensis, A. breviculmis, A. capillaris, A. foliata, A. cf. imberbis, A. mertensii, A. perennans s.l., A. stolonifera, A. tolucensis, Podagrostis trichodes). Agrostis cf. imberbis previamente conocido de Sudamerica austral, se presenta como un nuevo registro para Colombia y A. capillaris se presenta como un nuevo registro regional para Boyacá con el nombre de Agrostis stuebelii también lectotipificado. Se incluyen claves en inglés y español para distinguir las 15 especies de Agrostis y dos especies de Podagrostis citadas para Colombia.

New combinations and updated descriptions in Podagrostis (Agrostidinae, Poaceae) from the Neotropics and Mexico.

Based on morphological study and corroborated by unpublished molecular phylogenetic analyses, five grass species of high-mountain grasslands in Mexico, Central and South America, Agrostis bacillata, A. exserta, A. liebmannii, A. rosei, and A. trichodes, are transferred to Podagrostis and bring the number of species of this genus recognized in the New World to ten. The name Apera liebmannii is lectotypified and epitypified. We provide an updated genus description for Podagrostis, and updated species descriptions, images, and notes on the new combinations. The diagnostic characteristics differentiating Podagrostis from Agrostis are: a) palea that reaches from (2/3) ¾ to almost the apex of the lemma; b) florets that usually almost equal the length of the glumes or are at least ¾ the length of the glumes; c) rachilla extension present and emerging from under the base of the palea as a slender short stub (rudimentary or up to 1.4 mm long, sometimes obscure in most florets in P. rosei), smooth or scaberulous, glabrous or distally pilulose (hairs < 0.3 mm long); d) lemmas usually awnless, sometimes with a short straight awn 0.2-0.6 mm long, inserted medially or in the upper 1/3 of the lemma, not surpassing the glumes (awn well-developed, straight or geniculate and inserted in lower 1/3 of lemma, not or briefly surpassing glumes in P. rosei). We include a generic key to distinguish the species of Podagrostis from other similar genera in Latin America and a key to distinguish the species of Podagrostis now accepted as occurring in these areas.

ResumenBasado en estudios morfológicos y corroborado por datos no publicados de análisis filogenéticos, las cinco gramíneas de alta montaña de los pastizales en México, Centroamérica y Sur América Agrostis bacillata, A. exserta, A. liebmannii, A. rosei, y A. trichodes, son transferidos a Podagrostis incrementando a diez el número de especies en el nuevo mundo de este reconocido género. El nombre de Apera liebmannii también es lectotipificado y epitipicado. Proporcionamos una descripción actualizada del género Podagrostis, y descripciones actualizadas de las especies, imágenes y comentarios sobre las nuevas combinaciones. Las características diagnósticas que diferencian a Podagrostis de Agrostis son: a) pálea de 2/3-¾ hasta casi el ápice de la lemma; b) espiguillas, generalmente casi iguales en longitud a las glumas o de al menos ¾ la longitud de las glumas; c) extensión de la raquilla presente y emergiendo desde debajo de la base de la pálea como un trozo corto y delgado (desde rudimentario hasta de 1.4 mm de largo, a veces oscuro en la mayoría de los flósculos en P. rosei), liso o escabérulo, glabro o piloso distalmente (pelos < 0.3 mm de largo); d) lemmas generalmente sin arista, o a veces con arista corta y recta de 0.2­0.6 mm de largo, insertada medialmente o en el tercio superior de la lemma, sin sobrepasar las glumas (arista bien desarrollada, recta o geniculada e insertada en la parte inferior 1/3 de lemma, no o superando brevemente las glumas en P. rosei). Incluimos una clave para distinguir Podagrostis de otros géneros similares presentes en América Latina y una clave para distinguir las especies de Podagrostis ahora aceptadas por estar presentes en estas áreas.

Functional androdioecy in the ornamental shrub Osmanthus delavayi (Oleaceae).

Androdioecy is one of the rarest sexual systems among plants, characterized by males co-occurring with hermaphrodites. Osmanthus delavayi (Oleaceae), an ornamental shrub from southern China, is known to have both male and hermaphrodite individuals, but little is known regarding the breeding system of this species and whether it is functionally androdioecious, and how this potentially evolved. In this study, we explore the characteristics of the breeding system of O. delavayi through the study of phenology, sex ratio, floral organ morphology, pollen number, stigma receptivity, artificial pollination, pollinators, and gene flow within and between populations, while also discussing the evolution and maintenance of androdioecy within the genus. The proportion of males was less than 0.5 and the out-crossing index (OCI) was 5. Morphological androdioecy was observed, with hermaphrodite flowers having fertile pistils, while male flowers had degenerated pistils. Males and hermaphrodites both had large amounts of small and fertile pollen grains, although the pollen number of males was ca. 1.21 × more than that of hermaphrodites, and pollen was generally smaller. Self-pollination was found to produce a much lower fruit set than outcrossing under natural conditions. Gene flow between males and hermaphrodites within a population was greater (1.007) than that between populations (0.753). All these results indicate that O. delavayi is functionally androdioecious, which may be an intermediate state in the evolutionary transition from hermaphroditism to dioecy.

Parent psychology and the decision to delay childhood vaccination.

OBJECTIVE: The study of vaccine hesitancy identifies parental decisions to delay childhood vaccinations as an important public health issue, with consequences for immunization rates, the pursuit of nonmedical exemptions in states, and disease outbreaks. While prior work has explored the demographic and social underpinnings of parental decisions to delay childhood vaccinations, little is known about how the psychological dispositions of parents are associated with this choice. We analyze public opinion data to assess the role of psychological factors in reported parental decisions to delay childhood vaccination. RATIONALE: We anticipate that parents with certain psychological characteristics will be more likely to delay childhood vaccination. Specifically, we explore the roles of conspiratorial thinking, dispositions towards needle sensitivity, and moral purity; expecting that parents with high levels of any of these characteristics will be more likely to delay vaccinating their children. METHOD: In an original survey of 4010 American parents weighted to population benchmarks, we asked parents about delay-related vaccination behavior, demographic questions, and several psychological batteries. We then developed a vaccination delay scale and modeled delay as a function of conspiratorial thinking, needle sensitivity, moral purity, and relevant demographic controls. We then re-specified our models to look specifically at the predictors of delaying HPV vaccination, which has a low uptake rate in the United States. RESULTS: Controlling for other common predictors of hesitant behavior, we find that parents with high levels of conspiratorial thinking and needle sensitivity are more likely to report pursuing alternative vaccination schedules. When analyzing the specific decision by parents to delay HPV vaccination, we find that tendencies towards moral purity and, in turn, sexual deviance are also associated with vaccine seeking behavior. CONCLUSION: Parental decisions to delay childhood vaccinations are an important public health concern that are associated with conspiratorial thinking and needle sensitivity.

Páramo Calamagrostis s.l. (Poaceae): An updated list and key to the species known or likely to occur in páramos of NW South America and southern Central America including two new species, one new variety and five new records for Colombia.

Calamagrostis (syn. Deyeuxia), as traditionally circumscribed, is one of the most speciose genera from páramo grasslands of northwest South America and southern Central America and often dominates these high-elevation habitats. However, it remains difficult for researchers to accurately identify the species due to a lack of floristic treatments for most of the countries containing páramo, with the distribution of many species still very poorly known. In an effort to ameliorate this, we present an updated list and identification keys in English and Spanish (as electronic appendix) to the species of Calamagrostis s.l. known or likely to occur in the páramos of Peru, Ecuador, Colombia, Venezuela, Costa Rica and Panama. Fifty-four species are accepted, constituting 47 species currently circumscribed in Calamagrostis and seven species recently transferred to Deschampsia. Included within this are two new species, Calamagrostiscrispifolius and Deschampsiasantamartensis, which are described and illustrated. Both new species are found in páramos of the Sierra Nevada de Santa Marta (departamento Magdalena), on the northernmost tip of Colombia, with C.crispifolius also found in the Serrania de Perija on the border with Venezuela. Calamagrostiscrispifolius differs from all other species of Calamagrostis s.l. by the presence of strongly curled, readily deciduous leaf blades, amongst numerous other characteristics including open inflorescences with generally patent branches, small spikelets, (3.5-)4-5.5 mm long, with sessile florets and a rachilla prolongation reaching from 2/3 to almost the apex of the lemma, with short hairs (< 1 mm long). Deschampsiasantamartensis is similar to Deschampsiahackelii (=Calamagrostishackelii) from austral South America but differs by its broad, rigid and erect, strongly conduplicate blades, 1.5-2.5 mm wide when folded, ligules of innovations 0.5-1 mm long, truncate or obtuse, ligules of upper flowering culms 3-4 mm long, broadly shouldered with an attenuate central point, ellipsoid spike-like panicle, 3-5.5 long × 1.5-2.5 cm wide, lemma surfaces moderately to lightly scabrous between the veins, lemma apex acute to muticous, entire, rachilla extension often absent and inside of the floret often with hyaline shiny sinuous trichomes to 1 mm long, emerging from the base of the ovary. We also present a broader circumscription of the common species Deschampsiapodophora (=Calamagrostispodophora), with the new variety D.podophoravar.mutica described and illustrated. Deschampsiapodophoravar.mutica principally differs from var. podophora by florets lacking awns and larger habit i.e. multiple taller culms with longer and wider leaf blades forming tussocks, with inflorescences often held within sheaths. Nomenclatural changes are presented, with Deyeuxiamacrostachya newly synonymised under C.macrophylla and C.pittieri, C.pubescens and Deyeuxiapubescens newly synonimised under C.planifolia. Lectotypes are designated for Agrostisantoniana, Calamagrostispisinna, Deyeuxiamacrostachya and Deyeuxiasodiroana. We also document and give notes on five new records of Calamagrostis for Colombia: C.carchiensis, C.guamanensis, C.heterophylla, C.pisinna and C.rigida.

ResumenCalamagrostis (syn. Deyeuxia), como traditionalmente está delimitado, es uno de los géneros con mayor número de especies registradas para las zonas paramunas de Sudamérica y el sur de Centroamérica, en donde a menudo es un elemento dominante en hábitats de alta montaña tropical. Sin embargo, es aun difícil para investigadores identificar con precisión las especies de este género, principalmente por la falta de tratamientos florísticos para la mayoría de países que contienen páramo, con la distribución de muchas especies todavía poca conocida. Con el fin de realizar un aporte al conocimiento de las gramíneas tropicales, se presenta una lista actualizada de nombres y claves de identificación taxonómica en inglés y español (como appendice electrónico) de las especies de Calamagrostis s.l. conocidas o que probablemente se encuentran en los páramos de Perú, Ecuador, Colombia, Venezuela, Costa Rica y Panamá. Como resultado, 54 especies son aceptadas, de las cuales 47 especies están circunscritas en Calamagrostis y siete especies están recientemente transferidas a Deschampsia. Como novedades taxonómicas, se presentan dos nuevas especies, Calamagrostiscrispifolius y Deschampsiasantamartensis, las cuales están descritas e ilustradas. Estas nuevas especies crecen en páramos de la Sierra Nevada de Santa Marta (departamento de Magdalena), en la punta más al norte de Colombia, con C.crispifolius también presente en la Serranía de Perijá en la frontera con Venezuela. Calamagrostiscrispifolius difiere de las otras especies de Calamagrostis s.l. por la presencia de láminas foliares fuertemente crispadas y fácilmente caducas, entre otras características incluyendo inflorescencias abiertas con ramas generalmente patentes, espiguillas pequeñas, (3.5­)4­5.5 mm long., con antecios sésiles y una prolongación de la raquilla llegando desde 2/3 hasta casi el ápice de la lema, con tricomas cortos (< 1 mm long.). Deschampsiasantamartensis es parecida a Deschampsiahackelii (=Calamagrostishackelii) de Sudamérica austral, pero difiere por sus láminas foliares siendo anchas, rígidas y rectas, y fuertemente conduplicadas, 1.5­2.5 mm de ancho cuando plegada, lígulas de las innovaciones 0.5­1 mm long., truncadas u obtusas, lígulas de la parte superior de las cañas floríferas 3­4 mm long., anchas con un punto central atenuado, panojas elipsoides y espiciforme, 3­5.5 long. × 1.5­2.5 cm de ancho, superficies de las lemas moderada a levemente escabroso entre las venas, ápices de las lemas agudas a múticas, enteras, prolongación de la raquilla a menudo ausente, y la presencia de tricomas hialinos, brillantes, y sinuosos de hasta 1 mm long., los cuales salen de la base del ovario. Por último, se presenta una circunscripción más amplia de la especie común Deschampsiapodophora (=Calamagrostispodophora), con la nueva variedad D.podophoravar.mutica, la cual es descrita e ilustrada. Deschampsiapodophoravar.mutica difiere principalmente de la var. podophora porque en sus antecios las aristas están ausentes, y su hábito es más grande i.e. con múltiples cañas largas con láminas foliares más largas y anchas, con inflorescencias a menudo escondidas dentro de las vainas. Los cambios nomenclaturales que se presentan son Deyeuxiamacrostachya sinonimizada bajo C.macrophylla, y C.pittieri, C.pubescens y Deyeuxiapubescens sinonimizada bajo C.planifolia. Designamos los lectotipos para Agrostisantoniana, Calamagrostispisinna, Deyeuxiamacrostachya y Deyeuxiasodiroana. También documentamos y damos notas de cinco nuevos registros de Calamagrostis para la flora de Colombia: C.carchiensis, C.guamanensis, C.heterophylla, C.pisinna, y C.rigida.

Autism spectrum disorder, politics, and the generosity of insurance mandates in the United States.

The study of Autism Spectrum Disorder (ASD) in the United States has identified a growing prevalence of the disorder across the country, a high economic burden for necessary treatment, and important gaps in insurance for individuals with autism. Confronting these facts, states have moved quickly in recent years to introduce mandates that insurers provide coverage for autism care. This study analyzes these autism insurance mandates and demonstrates that while states have moved swiftly to introduce them, the generosity of the benefits they mandate insurers provide varies dramatically across states. Furthermore, our research finds that controlling for policy need, interest group activity, economic circumstances, the insurance environment, and other factors, the passage of these mandates and differences in their generosity are driven by the ideology of state residents and politicians-with more generous benefits in states with more liberal citizens and increased Democratic control of state government. We conclude by discussing the implications of these findings for the study of health policy, politics, and autism in America.

The Complete Plastid Genome of Magnolia zenii and Genetic Comparison to Magnoliaceae species.

Magnolia zenii is a critically endangered species known from only 18 trees that survive on Baohua Mountain in Jiangsu province, China. Little information is available regarding its molecular biology, with no genomic study performed on M. zenii until now. We determined the complete plastid genome of M. zenii and identified microsatellites. Whole sequence alignment and phylogenetic analysis using BI and ML methods were also conducted. The plastome of M. zenii was 160,048 bp long with 39.2% GC content and included a pair of inverted repeats (IRs) of 26,596 bp that separated a large single-copy (LSC) region of 88,098 bp and a small single-copy (SSC) region of 18,757 bp. One hundred thirty genes were identified, of which 79 were protein-coding genes, 37 were transfer RNAs, and eight were ribosomal RNAs. Thirty seven simple sequence repeats (SSRs) were also identified. Comparative analyses of genome structure and sequence data of closely-related species revealed five mutation hotspots, useful for future phylogenetic research. Magnolia zenii was placed as sister to M. biondii with strong support in all analyses. Overall, this study providing M. zenii genomic resources will be beneficial for the evolutionary study and phylogenetic reconstruction of Magnoliaceae.