Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 23
Filter
1.
Vet Rec ; 167(19): 739-43, 2010 Nov 06.
Article in English | MEDLINE | ID: mdl-21257509

ABSTRACT

Over a period of 11 years, 33 sheep or goat holdings lost their maedi-visna or caprine arthritis-encephalitis accredited status (mean 2.8 per year [0.09 per cent] of the accredited holdings in Great Britain). Of these, 22 sheep flocks and two goat herds eradicated the infection and regained their accredited status. In addition, 10 sheep flocks and two goat herds managed to eradicate infection, having joined the scheme with infected animals. In flocks and herds with a high initial prevalence of infection, the adoption of an indirect ELISA, with greater sensitivity than the agar gel immunodiffusion test, improved success rates. A strategy was devised to interpret the ELISA results depending upon the prevalence of infection at the time. Eighteen of the 33 flocks/herds (54.5 per cent) that had introductions of infection also owned non-accredited stock.


Subject(s)
Arthritis-Encephalitis Virus, Caprine/immunology , Goat Diseases/epidemiology , Lentivirus Infections/veterinary , Sheep Diseases/epidemiology , Visna-maedi virus/immunology , Animals , Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay/standards , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Goat Diseases/blood , Goat Diseases/diagnosis , Goats , Immunodiffusion , Lentivirus Infections/blood , Lentivirus Infections/epidemiology , Lentivirus Infections/prevention & control , Male , Sensitivity and Specificity , Sheep , Sheep Diseases/blood , Sheep Diseases/diagnosis , United Kingdom/epidemiology
2.
Vet J ; 174(3): 554-64, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17977035

ABSTRACT

The prevalence of verocytotoxin-producing Escherichia coli (VTEC) O157 in 12-30-month-old beef finishing cattle in Scotland was determined using 1g faeces samples enriched in buffered peptone water, followed by immunomagnetic separation (IMS) and isolation on sorbitol MacConkey agar with cefixime and tellurite supplement (CT-SMAC). A validated questionnaire was used to collect information that could be associated with the samples. Generalised Linear Models and Generalised Linear Mixed Models were used to identify factors associated with shedding both between and within groups. A total of 14,856 samples were collected from 952 farms, of which 1231 were positive for VTEC O157. Prevalence levels were calculated with 95% confidence intervals as follows: 7.9% (6.5%, 9.6%) of animals sampled were estimated to be shedding VTEC O157, while 22.8% (19.6%, 26.3%) of farms were estimated as having at least one animal shedding in the group sampled. The median percentage of animals shedding in positive groups was 25% (20%, 32%). An increased probability of a group containing a shedding animal was associated with larger numbers of finishing cattle, the presence of pigs on the farm, or the farm being classed as a dairy unit stocking beef animals. Farms that spread slurry on grazing land were more likely to have shedding animals, while those that spread manure were at lower risk. Groups with older animals were less likely to be identified as positive. There was no significant regional difference in group shedding probabilities, but the proportion of positive groups dropped over two successive years of the study. Higher mean levels of shedding in positive groups were associated with animals being housed rather than at pasture, and this effect was stronger in groups which had recently had a change in housing or diet. Farms with animals at pasture had lower mean prevalence where water was supplied from a natural source, as had farms with higher numbers of finishing cattle. There remained unexplained variability in mean prevalence levels on positive farms in different areas of Scotland.


Subject(s)
Cattle Diseases/microbiology , Escherichia coli Infections/veterinary , Escherichia coli O157/isolation & purification , Escherichia coli O157/metabolism , Shiga Toxins/metabolism , Animals , Cattle , Escherichia coli Infections/microbiology , Feces/microbiology , Prevalence , Scotland
3.
Appl Environ Microbiol ; 70(10): 5737-43, 2004 Oct.
Article in English | MEDLINE | ID: mdl-15466508

ABSTRACT

The distribution of Escherichia coli O157 in bovine feces was examined by testing multiple samples from fecal pats and determining the density of E. coli O157 in immunomagnetic separation (IMS)-positive fecal samples. The density of E. coli O157 in bovine feces was highly variable, differing by as much as 76,800 CFU g(-1) between samples from the same fecal pat. The density in most positive samples was <100 CFU g(-1), the limit of reliable detection by IMS. Testing only one 1-g sample of feces per pat with IMS may result in a sensitivity of detection as low as 20 to 50%. It is therefore probable that most surveys have greatly underestimated the prevalence of E. coli O157 shedding in cattle and the proportion of farms with shedding cattle. The sensitivity of the detection of E. coli O157 in bovine feces can be as much as doubled by testing two 1-g samples per pat rather than one 1-g sample.


Subject(s)
Cattle/microbiology , Escherichia coli O157/isolation & purification , Feces/microbiology , Animal Husbandry , Animals , Colony Count, Microbial/methods , Colony Count, Microbial/statistics & numerical data , Cross-Sectional Studies , Immunomagnetic Separation/statistics & numerical data , Monte Carlo Method , Scotland , Sensitivity and Specificity
4.
Lett Appl Microbiol ; 39(2): 207-14, 2004.
Article in English | MEDLINE | ID: mdl-15242463

ABSTRACT

AIMS: To determine the prevalence and levels of zoonotic agents in livestock wastes. METHODS AND RESULTS: A proportionally weighted survey was undertaken and livestock waste samples analysed quantitatively for Escherichia coli O157, pathogenic Listeria, Salmonella, Campylobacter, Giardia and Cryptosporidium. A significant proportion of wastes contained at least one zoonotic agent. Relationships were found between dry matter content and the presence and levels of some zoonotic agents. CONCLUSIONS: British livestock wastes contain measurable levels of the zoonotic agents that cause most cases of gastroenteritis in the UK. SIGNIFICANCE AND IMPACT OF THE STUDY: Animal wastes are disposed of by spreading to agricultural land used for the production of crops and livestock grazing. As British wastes are contaminated with significant levels of zoonotic agents, the practice may represent a way for pathogens to travel further up the food chain.


Subject(s)
Animals, Domestic/microbiology , Manure/microbiology , Zoonoses/microbiology , Animals , Campylobacter/chemistry , Campylobacter/isolation & purification , Colony Count, Microbial , Cryptosporidium parvum/chemistry , Cryptosporidium parvum/isolation & purification , Escherichia coli O157/chemistry , Escherichia coli O157/isolation & purification , Giardia lamblia/chemistry , Giardia lamblia/isolation & purification , Humans , Listeria/chemistry , Listeria/isolation & purification , Salmonella/chemistry , Salmonella/isolation & purification , United Kingdom/epidemiology , Virulence Factors/isolation & purification , Zoonoses/epidemiology
5.
J Appl Microbiol ; 95(1): 155-9, 2003.
Article in English | MEDLINE | ID: mdl-12807466

ABSTRACT

AIMS: To compare the sensitivity of two pre-enrichment broth media prior to immunomagnetic separation for the isolation of Escherichia coli O157 from cattle faeces. METHODS AND RESULTS: One-gram portions of 721 cattle faeces collected from 43 farms were pre-enriched in buffered peptone water containing vancomycin, cefixime and cefsulodin (BPW-VCC) and buffered peptone water without additives (BPW-WOA), respectively. A total of 137 samples were positive for E. coli O157: 127 pre-enriched with BPW-WOA and 89 pre-enriched in BPW-VCC. Representative isolates were tested for phage type, verotoxin and eae (E. coli attaching and effacing) gene sequences, resulting in the recognition of eight different types. All the E. coli O157 types recognized were isolated by both methods except for three different strains, each of which were isolated only on a single occasion: two by BPW-WOA and another by BPW-VCC. CONCLUSIONS: The results clearly demonstrate, under the conditions of this study, that BPW without antibiotics was the superior pre-enrichment medium for the isolation of E. coli O157 from cattle faeces. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of BPW-WOA in preference to BPW-VCC for the isolation of E. coli O157 from cattle faeces in future research and outbreak studies should lead to a higher number of positive isolates.


Subject(s)
Culture Media , Escherichia coli O157/isolation & purification , Feces/microbiology , Immunomagnetic Separation/methods , Animals , Anti-Bacterial Agents , Bacteriological Techniques/methods , Bacteriophage Typing/methods , Cattle , Cefixime , Cefsulodin , Disease Reservoirs , Sensitivity and Specificity , Vancomycin
6.
Epidemiol Infect ; 130(2): 301-12, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12729199

ABSTRACT

A study was designed to investigate management factors that might influence the shedding of verocytotoxin-producing Escherichia coli (VTEC) O157 by beef cows in Scotland, where there is a particularly high rate of human infection. Thirty-two herds were visited at least monthly over approximately 1 year for collection of fresh faecal pat samples and information on management factors. The faecal pat samples were tested for VTEC O157 by established culture and immunomagnetic separation methods. Questionnaires were completed at the monthly visits to record management factors. Data were analysed using both univariate and multi-factor (GLMM) analysis. Changes in the number of cows in a group, dogs, wild geese, housing, and the feeding of draff (distillers' grains) were statistically significant as risk factors. The event of calving appeared to reduce the likelihood of shedding. Any effects of weaning or turnout were not statistically significant. It appears that the rate of shedding of VTEC O157 is influenced by several factors but possibly the most important of these are the circumstances of animals being housed, or, when outside, the presence of wild geese.


Subject(s)
Cattle/microbiology , Escherichia coli O157/isolation & purification , Meat/microbiology , Shiga Toxins/biosynthesis , Animal Husbandry , Animals , Escherichia coli O157/pathogenicity , Female , Risk Factors , Seasons
7.
J Appl Microbiol ; 93(6): 944-53, 2002.
Article in English | MEDLINE | ID: mdl-12452950

ABSTRACT

AIMS: Strains of Verocytotoxin-producing Escherichia coli (VTEC) from Scottish beef cattle on the same farm were isolated during four visits over a period of eight months. Characteristics of these strains were examined to allow comparisons with strains of VTEC associated with human infection. METHODS AND RESULTS: Strains were characterized to investigate the relationship between these bovine isolates with respect to serotype, Verocytotoxin (VT) type, intimin-type, and presence or absence of the enterohaemolysin genes. VT genes were detected in 176 of 710 (25%) faecal samples tested using PCR, although only 94 (13%) VTEC strains were isolated using DNA probes on cultures. Forty-five different serotypes were detected. Commonly isolated serotypes included O128ab:H8, O26:H11 and O113:H21. VTEC O26:H11 and O113:H21 have been associated with human disease. Strains harbouring the VT2 genes were most frequently isolated during the first three visits to the farm and those with both VT1 and VT2 genes were the major type during the final visit. Of the 94 strains of non-O157 VTEC isolated, 16 (17%) had the intimin gene; nine had the gene encoding beta-intimin and seven strains had an eta/zeta-intimin gene. Forty-one (44%) of 94 strains carried enterohaemolysin genes. CONCLUSIONS: Different serotypes and certain transmissible characteristics, such as VT-type and the enterohaemolysin phenotype, appeared to be common throughout the VTEC population at different times. SIGNIFICANCE AND IMPACT OF THE STUDY: Detailed typing and subtyping strains of VTEC as described in this study may improve our understanding of the relationship between bovine VTEC and those found in the human population.


Subject(s)
Cattle Diseases/microbiology , Escherichia coli Proteins , Escherichia coli/metabolism , Shiga Toxins/biosynthesis , Adhesins, Bacterial/genetics , Animals , Base Sequence , Carrier Proteins/genetics , Cattle , Escherichia coli/classification , Escherichia coli Infections/microbiology , Feces/microbiology , Food Microbiology , Hemolysin Proteins/genetics , Humans , Molecular Sequence Data , Ribotyping , Scotland , Serotyping/methods
9.
Epidemiol Infect ; 126(1): 135-8, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11293673

ABSTRACT

An outbreak of E. coli O157 infection occurred in the Highland Region of Scotland in the summer of 1999. The source of the outbreak was traced to an untreated private water supply. All six cases identified arose in visitors to the area, and most had very limited exposure to the contaminated water. Permanent residents on the same supply were unaffected. The E. coli O157 isolates from the water, sheep faeces collected from around the source and the human stool samples were indistinguishable using pulsed field gel electrophoresis. Previously reported outbreaks of E. coli O157 linked to potable water supplies have resulted from structural or treatment failures, which allowed faecal contamination of source water. Here, contamination of the water supply and subsequent human infection was due to the use of an untreated, unprotected private water source in a rural area where animals grazed freely.


Subject(s)
Disease Outbreaks , Escherichia coli Infections/epidemiology , Escherichia coli O157 , Feces/microbiology , Water Microbiology , Water Supply , Adult , Animals , Child , Child, Preschool , Deer , Electrophoresis, Gel, Pulsed-Field , Escherichia coli Infections/transmission , Humans , Infant , Scotland/epidemiology , Sheep , Zoonoses
10.
Symp Ser Soc Appl Microbiol ; (29): 31S-37S, 2000.
Article in English | MEDLINE | ID: mdl-10880177

ABSTRACT

This overview places verocytotoxin-producing Escherichia coli (VTEC) in perspective with other E. coli types that cause disease in animals. VTEC O157 and other verocytotoxin-producing serotypes cause severe disease in man but to date, although other VTEC are found in animals, zoonosis appears to be associated with E. coli O157 only. The epidemiology of E. coli O157 in cattle has been studied in Scotland, and this work is described alongside current knowledge.


Subject(s)
Escherichia coli Infections/veterinary , Escherichia coli O157/metabolism , Shiga Toxins/metabolism , Animals , Cattle , Chickens , Disease Outbreaks , Dogs , Escherichia coli Infections/epidemiology , Escherichia coli Infections/microbiology , Humans , Mice , Rabbits , Scotland/epidemiology , Swine , Zoonoses/microbiology
11.
Vet Microbiol ; 64(2-3): 223-9, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10028174

ABSTRACT

A scheme to control and eradicate bovine virus diarrhoea (BVD) was initiated in 1994 in the Shetland Islands by local veterinary surgeons and funded by the Shetland Islands Council and Shetland Enterprise Company. Over a 3-year period every bovine animal on the islands was blood-sampled (heparinised) and laboratory tested using MAb-based ELISAs for BVD virus antibody and antigen detection for evidence of disease. A number of BVD virus positive animals (40) were found and culled. A total of 6150 animals were tested from 213 herds and 43% herds were found to be BVD naive. The remaining herds had experienced infection and contained many BVD antibody positive animals. Some repeat sampling of stock in infected herds determined further virus positive animals which were slaughtered and in 1997 the scheme ceased since it appeared that there were no persistent excretors present. The major risk to the Shetland Islands is from bought-in stock, especially animals which are imported in calf. It is vital that all bought-in animals are tested and proven to be free of BVD virus if these animals are in calf, the calves must be tested a birth to determine status. It is strongly advised that only bulls and bulling heifers or cows are bought into Shetland in future, thus, protecting the present stock. Continued surveillance will be required to claim eradication of BVD from Shetland.


Subject(s)
Bovine Virus Diarrhea-Mucosal Disease/prevention & control , Diarrhea Viruses, Bovine Viral/immunology , Agriculture , Animals , Antibodies, Monoclonal , Antibodies, Viral/blood , Antigens, Viral/blood , Bovine Virus Diarrhea-Mucosal Disease/epidemiology , Cattle , Disease Reservoirs , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Male , Pregnancy , Scotland/epidemiology , Seroepidemiologic Studies , Veterinarians
18.
19.
Vet Rec ; 124(15): 410-1, 1989 Apr 15.
Article in English | MEDLINE | ID: mdl-2728291
20.
Vet Rec ; 119(2): 31-4, 1986 Jul 12.
Article in English | MEDLINE | ID: mdl-3750766

ABSTRACT

Faeces samples were collected from 302 untreated calves on the day of onset of diarrhoea and from 49 healthy calves at 32 farms experiencing outbreaks of diarrhoea. At least four diarrhoeic calves were sampled on each farm, and samples were examined for rotavirus, coronavirus, cryptosporidium, enterotoxigenic Escherichia coli and Salmonella species. Although all these enteropathogens were excreted more frequently by the diarrhoeic than by the healthy calves, the difference was significant overall only for rotavirus. Rotavirus was excreted by 18 per cent of healthy calves, coronavirus by 4 per cent, cryptosporidium by 14 per cent, and no enterotoxigenic E coli or Salmonella species were detected. The most common enteropathogen in diarrhoeic calves was rotavirus, which was excreted by more than half the diarrhoeic calves on 18 farms. Coronavirus was excreted at a similar high prevalence on one farm, cryptosporidium on five farms and enterotoxigenic E coli on three farms. Concurrent infection with two or more microorganisms occurred in 15 per cent of diarrhoeic calves. There was no difference in the isolation rate of campylobacters between diarrhoeic and healthy calves.


Subject(s)
Cattle Diseases/etiology , Diarrhea/veterinary , Feces/microbiology , Animals , Cattle , Cattle Diseases/microbiology , Diarrhea/etiology , Diarrhea/microbiology
SELECTION OF CITATIONS
SEARCH DETAIL
...