ABSTRACT
We studied the possibility of performing peripheral blood stem cell (PBSC) harvests during the course of ABVD therapy by adding G-CSF to the treatment regimen. Six patients with high-risk Hodgkin's disease (HD) (5 untreated cases with bulky mass and 1 relapsed case) received G-CSF (5 micrograms/kg) subcutaneously from day 8 to day 13 of their first course of ABVD treatment; the numbers of CD34+ cells and CFU-GM were monitored. PBSC harvests were performed on day 12 and day 13 of subsequent ABVD plus G-CSF treatment courses. For all patients tested, we were able to harvest CFU-GM (3.78 +/- 1.19 x 10(5) colonies/kg) for peripheral blood stem cell transplants (PBSCT) by performing 2 to 4 cycles. of apheresis, without any modification to the original ABVD protocol. These findings suggest that ABVD plus G-CSF therapy is a strong candidate for the treatment of patients with high-risk HD who may undergo autologous PBSCT.
Subject(s)
Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Blood Component Removal , Granulocyte Colony-Stimulating Factor/administration & dosage , Hematopoietic Stem Cell Transplantation , Hematopoietic Stem Cells/cytology , Hodgkin Disease/drug therapy , Adult , Bleomycin/administration & dosage , Dacarbazine/administration & dosage , Doxorubicin/administration & dosage , Female , Hematopoietic Stem Cell Mobilization , Humans , Male , Vinblastine/administration & dosageABSTRACT
The amount of phosphoric acid liberated from ATP by Ca2+ (Mg2+)-ATPase in microsomal fraction of guinea-pig thoracic aorta decreased with decreasing concentrations of calcium ions from 20.0 to 2.5 mM in the mixture of the enzyme and substrate. When CaCl2 (2.5 mM) and MgCl2 (5.0 mM) were present in the substrate, both nitroglycerin (0.1 to 1.0 mM) and SIN-1 A (a molsidomine derivative, 0.05 to 1.0 mM) increased the liberated phosphoric acid in a concentration-dependent manner. The contractile tension of smooth muscle prepared from guinea-pig thoracic aorta, which was previously increased by the pretreatment with prostaglandin F2 alpha (5.0 microM), was relaxed by both nitroglycerin and SIN-1 A (0.01 to 100 microM each) in a concentration-dependent manner. From the results, it is assumed that the stimulation of Ca2+ (Mg2+)-ATPase [Ca2+-pump ATPase] activity induced by nitroglycerin and SIN-1 A in the microsome of thoracic aorta takes part in the relaxation of contractile tension in the tissue.
