[Effect of intraoperative lavage of the large intestine with hyperoxygenated solution on the healing of large intestinal anastomosis]. / Vliianie intraoperatsionnogo lavazha tolstoi kishki giperoksigenirovannym rastvorom na zazhivlenie tolsto-tolstokishechnogo anastomoza.

In the experiment on 80 rats, the intraoperative lavage of the colon (IL) in its perforation and acute obstructive ileus was performed. Lavage was performed before wound closure and placing the anastomosis. In using IL with hyperoxigenated solution, the favourable course of the postoperative period, increase in number of the animals survived, decrease in incidence of complications were noted. The wound and intestinal anastomosis healed by first intention. The method is recommended for the use in clinical practice.

[Perivascular lymphoid nodules]. / Perivaskuliarnye limfoidnye uzelki.

As a result of investigation of lymphoid formations in the walls of the human tracheobronchial tree and the pulmonary stroma by means of morphological and morphometrical techniques, certain regularity in arrangement of the perivascular lymphoid noduli has been stated along the course of the lympho- and hemomicrocirculatory bed links. The periarterial lymphoid noduli of the tracheobronchial tree have certain morphological similarity and some differences, when comparing them with usual lymphoid noduli.

[The morphofunctional changes in the perivascular and lymphoepithelial nodes of the respiratory organs following antigenic stimulation]. / Morfofunktsional'nye izmeneniia perivaskuliarnykh i limfoépitelial'nykh uzelkov organov dykhaniia posle antigennogo stimulirovaniia.

Morphological, experimental-immunological methods with mathematical analysis were used in 108 Wistar line rats in the pre- and postnatal ontogenesis to study the lymphoid structures of the trachea, bronchi and stroma of the lungs. Two types of lymphoid nodules were detected in the respiratory organs of rats and man: 1. perivascularly located in the wall of the trachea, bronchi and stroma of the lungs; 2. lymphoid nodules of the mucous membrane of the respiratory ways which have a different structure. The former are related to a common immune system of the internal milieu, the latter--to a common immune system of the mucous membranes. Antigenic stimulation revealed that formation of lymphoid structures is enhanced and more rapidly terminated as to functional maturation. Lymphoid structures play a decisive role in local immunity.

[The age-related characteristics of the lymphoid structures in the human respiratory organs]. / Vozrastnye osobennosti limfoidnykh struktur organov dykhaniia cheloveka.

It was found that the wall of the tracheo-bronchial tree and the lung stroma of man has two types of nodules: those located in the mucous membrane and adjoining the epithelium and those located in the fibrous-cartilagenous part of the wall of the trachea, bronchi and stroma of the lungs. The latter serve the purpose of defense of the internal medium of the body. It was established that lymphoid nodules of the respiratory organs undergo age-related involution. According to the authors' classification perivascular lymphoid nodules and lymphoid nodules of the mucous membrane of the tracheo-bronchial tree belong to a single immune system of the internal medium and mucous membranes participating in immune homeostasis.

[Experimental characteristics of the immune response of lymphoid organs to fungal and staphylococcal antigens]. / Osobennosti immunnogo otveta limfoidnykh organov na gribkovyi i stafilokokkovyi antigeny v éksperimente.

Increased proliferative and functional activities of the lymphoid organs and a high mitotic activity of the lymphocyte blast forms were recorded by autohistoradiography (3H thymidine and 35S-labeled sulfate) in 60 guinea pigs in acute experiments (a challenge with staphylococcal or fungal antigen). Degeneration of the lymphoid tissue and substitution of the lymphoid tissue with fatty tissue were observed in a chronic experiment. Discoordination of the immune response consisted in depression of the T-component, with the B-component retaining high activity; these shifts were the most manifest after challenge with fungal antigen.

[Duration of the mitotic cycle periods of lymphoid cells from the respiratory organs and the effect of the intranasal administration of gamma-globulin]. / Dlitel'nost' periodov mitoticheskogo tsikla kletok limfoidnoi populiatsii organov dykhaniia i vliianie intranazal'nogo vvedeniia gamma-globulina.

Parameter studies of mitotic cycles of the lymphoid population in the rats respiratory system showed that after gamma-globulin injection to the respiratory system the main mechanism of cellular division consists of synchronized entry of cells to the mitotic cycle, shortening of the parameters and time of cellular regeneration.

[Effect of intratracheal gamma-globulin administration on the mitotic activity and proliferative pool of lymphoid cells of the respiratory tract]. / Vliianie intratrakheal'nogo vvedeniia gamma-globulina na mitoticheskuiu aktivnost' i proliferativnyi pul limfoidnykh kletok organov dykhaniia.

After injection of gamma-globulin into respiratory tracts of rats a proliferative pool of lymphoid cells in the lungs increases and remains unchanged in the spleen. The increase in the proliferative pool was accompanied by a decrease in a mitotic cycle in the S-period. Immunization was followed by intensification of division processes in a group of medium lymphocytes and processes of blast cells differentiation.

[Morphofunctional changes in the lymphoid tissue of the respiratory organs in gamma-globulin administration]. / Morfofunktsioanl'nye izmeneniia limfoidnoi tkano organov dykhaniia pri vvedenii gamma-globulina.

By means of macro-microscopic, histologic, radioautographic and immunomorphologic methods, three forms of the lymphoid tissue structural organization have been revealed in the rat respiratory organs: a lymphoid cluster, a lymphoid follicle and a lymphoepithelial nodule. After gamma-globulin is administered intranasally, the ratio of the lymphoid cells in the organs mentioned changes: the number of small lymphocytes decreases, while that of middle, large lymphocytes and blast cells increase. The lymphoid follicles consist of a central and a marginal zones, in the lymphoepithelial nodules the subepithelial and paranodular zones also exist. The gamma-globulin administration stimulates DNA synthesis by the cells of the lymphoid type and their proliferation; that results in increasing number of the antibody-synthesizing cells and is accompanied with an increasing titre of antibodies in washing from the bronchial tree. At a repeated stimulation, the processes are more intensive and develop more quickly.

[Morphofunctional changes in the glands of the tracheobronchial system following gamma globulin administration]. / Morfofunktsional'nye izmeneniia zhelez trakheobronkhial'noi sistemy posle vvedeniia gamma-globuliny.

Morpho-functional changes and synthesis of carbohydrate compounds in the mucous membrane of the rat and cat trachea and bronchi have been studied 2, 4, 7, 14 and 24 days after a single and twice administration of gamma-globulin into the respiratory tract and after repeated intrapleural and intramuscular administration following sensibilization with a complete Freund's adjuvant. Dynamic of the changes observed depends, to a great extent, on the mode and multiplicity of administration of the adjuvant. When gamma-globulin is administered into the respiratory tract or intrapleurally, a more active incorporation of 3H-glucose and 35S-sodium sulfate into chondroitinsulphate A, C, sialic acids and glycogen, which are synthesized by cells of the tracheobronchial system glands, occurs. In 14-24 days after gamma-globulin has been administered by any mode, sulfated glycosamineoglycans (of chondroitinsulfate B and heparin type), as well as proteoglycans and sialic acids resistive to treatment with sialidase are accumulating in discharge from the glands. The accumulation of the sulfated glycosamin sulfates is accompanied with an increased level of serum antibodies specific for the administered antigen.

[Radioautographic study of acid glycoprotein synthesis by tracheal and bronchial glands during ontogeny]. / Radioautograficheskoe issledovanie sinteza kislykh glikoproteinov zhelezami trakhei i bronkhov v ontogeneze.

The synthesis of acid glycoproteins in the glands of the respiratory pathway has been studied radioautographically by means of sodium sulfate in the cat ontogenesis. 35S-sulfate incorporation into the fetal gland has been stated to increase by the time of delivery. After birth and in young kittens, 35S-sulfate incorporation into the gland decreases. Acid glycoproteins are intensively synthesized by the tracheal and bronchial gland in young and adult animals. In old animals this process is lowered. The synthesis of acid glycoproteins by the tracheal and bronchial glands in ontogenesis changes both quantitatively and qualitatively.

[Histoautoradiographic study of sulfated mucopolysaccharides in glandular epithelial cells of the lower respiratory passages]. / Gistoavtoradiograficheskoe izuchenie sul'fatirovannykh mukopolisakharidov kletok zhelezistogo épiteliia nizhnikh dykhatel'nykh putei

It is established that mucous and serous cells of trachea and bronchia uptake radio-active sulphate with different rate. The intensity of radioactive sulphate incorporation by the mucous cells is higher than by the serous ones. The greater intensity of the incorporation is observed 12-24 hours after isotope's injection.