ABSTRACT
The study was an attempt to correlate phenotypic pathogenetic determinants of clinical Candida albicans strains with their genotype as determined by PCR fingerprinting. A total of 25 C. albicans strains was tested. Adherence capacity, hydrophobicity and proteinase production were compared with the genotypes of the particular Candida strains. The fungal strains represented eleven genotypes. No correspondence relationship was found between genotype and the markers of pathogenicity.
Subject(s)
Candida albicans/genetics , Candida albicans/pathogenicity , Candida albicans/classification , Candida albicans/isolation & purification , Genotype , Humans , PhenotypeABSTRACT
Evaluation regarded adherence of a strain of Candida albicans to epithelium of cheek mucous membrane, obtained from 31 children aged between one and 14 years with disturbance of immunity and from 10 healthy children. All children were tested immunologically by determination of total leukocytosis, granulocytosis, absolute lymphocytosis, active and total rosette tests, spontaneous and stimulated nitroblue-tetrazolium (NBT) tests and immunoglobulin A, G and M levels in serum. Adherence of C. albicans in vitro of epithelial cells of cheek mucous membrane was significantly higher in children with disturbance of immunity as compared with healthy control group (p < 0.001).
Subject(s)
Candida albicans/physiology , Immune System Diseases/microbiology , Mouth Mucosa/microbiology , Adolescent , Cells, Cultured , Cheek , Child , Child, Preschool , Female , Humans , Infant , MaleABSTRACT
An influence of mannan++, its component methyl-D-mannopyranoside+ and N-acetylglucosamine on in vitro adhesion of Candida albicans strains to buccal mucosal epithelium was studied. These substances inhibited adhesion when added to adherence test in a concentration of 10 mg/ml and 25 mg/ml despite whether were added to the test incubation medium or when preincubated with fungi or epithelial cells. Preincubation of fungal cells and epithelial cells with mannan had no influence on attachment; preincubation of epithelial cells with methyl-D-mannopyranoside+ and N-acetylglucosamine decreased adherence significantly. On the other hand preincubation of fungal calls with methyl-D-mannopyranoside+ increased their adhesive properties, having no influence on adherence after preincubation of fungi with N-acetylglucosamine.
Subject(s)
Acetylglucosamine/physiology , Candida albicans/physiology , Mannans/pharmacology , Mouth Mucosa/microbiology , Adhesiveness , Candida albicans/ultrastructure , Cell Wall/physiology , Epithelium/microbiology , Humans , In Vitro Techniques , Methylmannosides/pharmacology , Receptors, Virus/physiologyABSTRACT
The aim of this study was to evaluate hydrophobicity of fungal strains of Candida sp. isolated from clinical materials of patients with fungal infections. Two hundred and eighty one strains of C. albicans and 29 strains belonging to the other species of Candida were tested in salt aggregation test (SAT). Strong hydrophobicity (autoaggregation in the test) was found in 29.4% of tested strains. The majority of them was isolated from vagina. In 20 randomly selected strains hydrophobicity was measured not only by salt aggregation test but also by hydrophobic interaction chromatography (MIC). The ability to attach to buccal epithelial cells in vitro was compared between 50 strongly hydrophobic strains and 30 with low hydrophobicity. The former strains attached significantly stronger (p less than 0.001).
