1.
Analyst
; 141(18): 5329-38, 2016 Sep 21.
Article
in English
| MEDLINE
| ID: mdl-27332112
ABSTRACT
Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (â¼5.5 µm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (â¼0.4 µm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR(-/-) mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.