1.
IUCrJ
; 4(Pt 1): 50-64, 2017 Jan 01.
Article
in English
| MEDLINE
| ID: mdl-28250941
ABSTRACT
The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes (CADEE) is presented which allows a drastic reduction in the time necessary to prepare and analyze in silico semi-automated directed evolution of enzymes. A pedagogical example of the application of CADEE to a real biological system is also presented in order to illustrate the CADEE workflow.