ABSTRACT
The influences of both being in a supine position for a prolonged period and food intake on cardiovascular variables were studied under clinical-pharmacological test conditions. In a randomized crossover design study without drug or placebo administration, 6 healthy male volunteers received a light standard meal before and during test A and fasted in test B. In both tests, while they were continuously supine for more than 8 h, a synchronous recording of cardiovascular variables was done at 24, 26 and 28 min after starting the supine position (first recordings) and 25 times from 2 to 480 min after the first recordings. Using a multifactorial statistical analysis, each parameter was evaluated regarding the factors eating and time of supine recording. Eating led to a significant decrease in diastolic and mean blood pressure, PQ time and QS2 time, a downward trend in systemic vascular resistance and an upward trend in systolic blood pressure and cardiac output. When the subjects remained in a supine position for prolonged periods, significant increases in systolic, diastolic, mean blood pressure and systemic vascular resistance were noted as well as significant decreases in cardiac output and QS2 time. Thus, eating and remaining in a supine position for prolonged periods should be considered as sources of bias in clinical-pharmacological studies on cardiovascular drug effects and accompanying placebo controls.
Subject(s)
Blood Pressure/physiology , Eating/physiology , Heart Rate/physiology , Supine Position/physiology , Adult , Cardiac Output/physiology , Cross-Over Studies , Data Interpretation, Statistical , Humans , Male , Time Factors , Vascular Resistance/physiology , Young AdultABSTRACT
OBJECTIVE: In this study the effect of locally administered trapidil on human hand veins was examined. SUBJECTS: 10 healthy male volunteers aged 20 - 30 years were included. METHOD: The dorsal hand vein compliance technique was used. In a crossover design the influence of locally infused trapidil (mainly 5 - 400 microg/min) on hand veins preconstricted with either norepinephrine (adrenoceptor agonist) or dinoprost (prostaglandin F2alpha) was investigated. Preconstriction reduced the vein diameter by about 80% with continuous local infusion of individually determined doses of norepinephrine in the range 11 - 1,000 ng/min and dinoprost in the range 90 - 5,600 ng/min. Blood pressure, cardiac function (electrocardiogram) and skin temperature of the hand infused were monitored. RESULTS: Locally applied trapidil produced a dose-dependent dilation of hand veins preconstricted with norepinephrine and dinoprost. The corresponding ED50 values of trapidil did not differ significantly on an intraindividual comparison. Clinically important side effects with the drugs used were not observed. CONCLUSIONS: The results indicate that trapidil has a direct dilating action on superficial veins in humans. This effect is apparently achieved without involvement of adrenoceptors or prostanoid receptors in venous smooth muscle.
Subject(s)
Hand/blood supply , Trapidil/pharmacology , Vasoconstriction/drug effects , Vasodilator Agents/administration & dosage , Veins/drug effects , Adult , Cross-Over Studies , Dinoprost/pharmacology , Dose-Response Relationship, Drug , Humans , Infusions, Intravenous , Male , Norepinephrine/pharmacology , Reference Values , Trapidil/administration & dosage , Trapidil/adverse effects , Vasoconstrictor Agents/pharmacology , Vasodilator Agents/adverse effects , Vasodilator Agents/pharmacologyABSTRACT
There has been limited experience with routine therapeutic monitoring of mycophenolic acid (MPA; CAS 24280-93-1), which is the active metabolite of the new immunosuppressive prodrug mycophenolate mofetil (MMF; CAS 115007-34-6). MMF was introduced with recommendation for fixed oral dosing (1 g twice daily) in combination with cyclosporin A (CSA) and a glucocorticoid for the prevention of renal allograft rejection. In the course of routine CSA monitoring a MPA monitoring was performed in adult renal transplant patients receiving MMF in combination with CSA and methylprednisolone (MEP). For 30 consecutive patients with 234 plasma samples the relationship of MMF doses used and MPA plasma through levels estimated at steady state (C88 min) to clinical outcome was evaluated retrospectively. The MPA concentrations were determined with the enzyme-multiplied immunoassay technique (EMIT mycophenolic acid assay) on a Cobas Mira Plus analyzer. The within-run (n = 10) and between-run (n = 10) coefficients of variation were 3.6%, 3.5%, 3.1% and 3.6%, 5.1%, 6.7% analysing three MPA level plasma controls (1.25 mg/l, 7.5 mg/l, 12.5 mg/l), respectively. The data analysis of the MPA plasma trough levels resulted in a high variability between patients (0.3 to 3.4 mg/l) received the recommended fixed MMF dose (2 g/day). There was a higher incidence of adverse reactions with increasing MPA plasma trough levels (2.13 +/- 1.35 mg/l in 13 patients with side effects versus 1.53 +/- 0.67 mg/l in 17 patients without side effects; p < 0.001), regardless of reduction of MMF dose (1.77 +/- 0.3 g/day versus 1.89 +/- 0.2 g/day; NS), respectively. No acute rejection episodes occured under MMF administration in combination with CSA and MEP. The study shows that the to date recommended MMF dose resulted in individual, quite different MPA plasma trough levels, which were associated with incidence of side effects rather than the MMF doses. Therefore, monitoring of plasma MPA trough levels and individual dose adjustment could be helpful to reduce the incidence of adverse reactions and to increase the safety of MMF therapy.
ABSTRACT
The possible pharmacokinetic interaction between the new immunosuppressive mycophenolate mofetil (MMF) and tacrolimus (TAC), respectively, was assessed by comparing routinely estimated mycophenolic acid (MPA) plasma trough levels of 15 consecutive renal transplant patients receiving MMF in combination with methylprednisolone (MEP) and cyclosporin A (CSA, 10 patients) or in combination with MEP and tacrolimus (TAC, 5 patients). Coadministration of TAC instead of CSA resulted in a significant increase of mean MPA plasma trough levels [3.4 +/- 1.3 microg/mL (n = 22) versus 1.87 +/- 1.1 microg/mL (n = 57); p < 0.001], despite lower MMF doses [1.5 +/- 0.5 g/d versus 1.7 +/- 0.3 g/d (not statistically significant)]. This elevation in MPA levels is possibly caused by an interaction between MMF and TAC and could lead to a recommendation to monitor MPA plasma levels with appropriate dose adjustment.
Subject(s)
Immunosuppressive Agents/pharmacokinetics , Kidney Transplantation/physiology , Mycophenolic Acid/analogs & derivatives , Mycophenolic Acid/blood , Tacrolimus/pharmacokinetics , Adult , Anti-Inflammatory Agents/pharmacology , Cyclosporine/pharmacology , Dose-Response Relationship, Drug , Drug Interactions , Female , Humans , Male , Methylprednisolone/pharmacology , Middle Aged , Mycophenolic Acid/pharmacokineticsSubject(s)
Anti-Infective Agents , Anti-Bacterial Agents , Drug Prescriptions , Drug Utilization , Female , Germany , Humans , MaleABSTRACT
The synthesis of the vasodilator prostacyclin (PGI2) depends on the intracellular calcium concentration. For this reason the purpose of this study was to document the effects of verapamil (1), a slow calcium channel blocking agent on venous tissue. Human varicose veins were studied which were removed by a stripping operation. The veins were investigated ex vivo and in vitro. For ex vivo studies 11 patients were given 0.01 g 1 i.v. during the operation. In vitro experiments were carried out by an addition of 22 mumol/l 1 to the incubate. The synthesis of PGI2 was studied according to the inhibition of the ADP induced platelet aggregation without and with addition of substrate (prostaglandin endoperoxide H2). 1 did not induce changes in the synthesis of PGI2, neither after administration to patients nor after addition to the incubation fluid compared to control vessels without addition of 1.
Subject(s)
Epoprostenol/biosynthesis , Veins/metabolism , Verapamil/pharmacology , Adenosine Diphosphate/pharmacology , Humans , In Vitro Techniques , Platelet Aggregation/drug effects , Varicose Veins/metabolism , Veins/drug effectsABSTRACT
In continuation of investigations on primary hyperlipidaemias, we determined serum thromboxane (TX) B2 and prostaglandin (PG) F2 alpha after standardized blood clotting in patients without hyperlipidaemia and without (group 1, n = 11) or with coronary heart disease (CHD; group 2, n = 5), in patients with familial combined hyperlipoproteinaemia and without (group 3, n = 4) or with CHD (group 4, n = 5), as well as in patients with familial hypercholesterolaemia and CHD (group 5, n = 5). TXB2 was detected by gas chromatography and PGF2 alpha by means of radioimmunoassay. The TXB2 level did not differ significantly between the groups, but there was a tendency to higher values in hyperlipidaemia, while in group 5 the level tended to decrease with rising serum LDL-cholesterol and in group 3 it tended to increase with rising serum apolipoprotein B. The PGF2 alpha level was significantly lower in group 4 than in groups 1 and 3. It showed in group 5 a negative correlation with serum LDL-cholesterol and in group 3 a positive correlation with serum triglycerides.
Subject(s)
Dinoprost/blood , Hyperlipidemia, Familial Combined/blood , Hyperlipoproteinemia Type II/blood , Lipids/blood , Thromboxane B2/blood , Adult , Aged , Apolipoproteins A/blood , Apolipoproteins B/blood , Cholesterol/blood , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Coronary Disease/blood , Coronary Disease/genetics , Female , Humans , Male , Middle Aged , Triglycerides/bloodSubject(s)
Adrenergic beta-Antagonists/pharmacology , Propanolamines/pharmacology , Propranolol/pharmacology , Thromboxane B2/blood , Adrenergic beta-Antagonists/administration & dosage , Blood Pressure/drug effects , Chromatography, Gas , Heart Rate/drug effects , Humans , Injections, Intravenous , Propanolamines/administration & dosage , Propranolol/administration & dosage , Reference ValuesABSTRACT
In a prospective randomized trial the anti-thrombotic effect of dipyridamole was studied in 64 patients after cadaveric kidney allotransplantation. The frequency of early graft function was significantly higher in the control group, whereas the incidence of arterial and venous thromboses were not different. One-year graft survival could not be improved by dipyridamole. Therefore, dipyridamole should not be recommended in cadaveric kidney allotransplantation.
Subject(s)
Dipyridamole/therapeutic use , Graft Occlusion, Vascular/drug therapy , Graft Rejection/drug effects , Kidney Transplantation/immunology , Cadaver , Humans , Infusions, Intravenous , Kidney Function Tests , Prospective Studies , Randomized Controlled Trials as Topic , Renal Artery/surgery , Renal Veins/surgeryABSTRACT
Trapidil was given orally (200 mg, Rocornal dragees 100 mg) and intravenously (100 mg) to 12 healthy volunteers. Serum samples were analyzed for trapidil by HPLC. Complete bioavailability was obtained and a mean absorption time of 66.4 min was calculated using the method of curve moments.
Subject(s)
Pyrimidines/pharmacokinetics , Trapidil/pharmacokinetics , Administration, Oral , Adolescent , Adult , Biological Availability , Humans , Injections, Intravenous , Male , Tablets , Trapidil/administration & dosageABSTRACT
The influence of pentoxifylline on the synthesis of prostacyclin (PGI2) was studied using human varicose veins removed by a stripping operation. Patients got 100 mg pentoxifylline iv. during the operation. The synthesis of PGI2 was studied according to the inhibition of the ADP-induced platelet aggregation without and with addition of substrate (100 ng prostaglandinendoperoxide H2) to the incubation fluid. There were no changes in the synthesis of PGI2 compared to control values.
Subject(s)
Epoprostenol/biosynthesis , Muscle, Smooth, Vascular/metabolism , Pentoxifylline/pharmacology , Saphenous Vein/metabolism , Theobromine/analogs & derivatives , Varicose Veins/metabolism , Humans , Kinetics , Muscle, Smooth, Vascular/drug effects , Saphenous Vein/drug effectsABSTRACT
In 17 patients with cardiac extrasystoles, prostaglandin (PG) F2a (= alpha) administered intravenously at consecutive infusion rates of 25 to 100 micrograms/min showed the following effects in a dose-dependent manner: prolongation of total electromechanical systole, pre-ejection period, and left ventricular ejection time, indicating a negative inotropic activity, increase in systolic and diastolic blood pressure, and incidence of side effects. An additional 2 patients were excluded from the uniform evaluation because the PGF2a dosage was changed owing to a clear antiarrhythmic action in one and serious side effects in the other. The results supplement clinical-pharmacological findings with PGF2a.
Subject(s)
Anti-Arrhythmia Agents , Cardiac Complexes, Premature/drug therapy , Dinoprost/therapeutic use , Heart/physiopathology , Blood Pressure/drug effects , Cardiac Complexes, Premature/physiopathology , Carotid Arteries/drug effects , Carotid Arteries/physiopathology , Dinoprost/adverse effects , Dose-Response Relationship, Drug , Female , Heart/drug effects , Hemodynamics/drug effects , Humans , Male , Myocardial Contraction/drug effects , Pulse/drug effects , Systole/drug effectsABSTRACT
The influence of trapidil on serum levels and cardiac effects of digoxin was investigated in 10 healthy men, intraindividually compared with placebo. Each subject took digoxin orally for 9 days (0.375 mg daily on days 1 to 8, 0.25 mg on day 9) either combined with trapidil (400 mg daily on days 1 to 8, 200 mg on day 9) or combined with placebo in a cross-over design. Trapidil failed to change significantly the steady-state serum digoxin concentrations on day 9 of treatment. It did also not alter significantly the following digoxin effects: shortening in QS2c time indicating the cardiotonic digoxin effect, shortening in QTc time, increase in PTQ index. But trapidil prevented the negative chronotropic digoxin effect.
Subject(s)
Digoxin/blood , Pyrimidines/pharmacology , Trapidil/pharmacology , Adult , Digoxin/pharmacology , Double-Blind Method , Drug Interactions , Humans , Male , Myocardial Contraction/drug effectsABSTRACT
The conditioning of the donor by 1.0 microgram/kg X min Iloprost over 15 minutes leads to a considerable inhibition of the aggregation of thrombocytes in the domestic pig. 160% more ADP had to be added than before the application of Iloprost. When 0.5 microgram/ml Iloprost are added to the perfusion fluid and to the reperfusion fluid 0.22 microgram/ml and 0.27 microgram/ml, respectively, are to be proved in the venous efflux. Whether or not the difference is absorbed or metabolized must remain open. During the 72-hour storage of the kidney in a Euro-Collins-solution with 0.5 microgram/ml Iloprost after 24 hours a decrease of 0.34 microgram/ml, after 48 hours to 0.33 microgram/ml and after 72 hours to 0.20 microgram/ml takes place. Thus Iloprost is present in an effective concentration also after 72 hours. The decrease to one plateau rather speaks for a consumption by active metabolic effect or absorption than for a chemical decomposition.
Subject(s)
Epoprostenol/pharmacology , Kidney Transplantation , Organ Preservation/methods , Platelet Aggregation/drug effects , Animals , Epoprostenol/metabolism , Iloprost , SwineABSTRACT
Ten healthy and twenty diabetic volunteers (type 1) received 15 capsules (à 450 mg) cod liver oil for 2 weeks daily in addition to a "normal" diet. The levels of eicosapentaenoic acid in the plasma phospholipids of both groups were increased after the treatment. The inhibition of the prostacyclin formation by LDL was diminished when the LDL was isolated after the treatment in comparison to LDL taken in the same concentration and from the same donors before it. The thromboxane B2 (TXB2) synthesis capacity of clotting whole blood, thrombin-induced TXB2 formation by platelets as well as the 15(S)-hydroxy-11 alpha,9 alpha-epoxymethano-5Z, 13E-prostadienoic acid-induced platelet aggregation were not altered by the treatment in healthy volunteers, whereas in diabetics the TXB2 formation capacity of clotting whole blood was decreased after the treatment in comparison with before it.
Subject(s)
Blood Platelets/metabolism , Cod Liver Oil/administration & dosage , Diabetes Mellitus, Type 1/blood , Epoprostenol/antagonists & inhibitors , Fatty Acids/blood , Fish Oils/administration & dosage , Lipoproteins, LDL/blood , Thromboxane B2/blood , Adult , Bleeding Time , Epoprostenol/blood , Female , Humans , Male , Malondialdehyde/blood , Phospholipids/blood , Platelet Aggregation/drug effects , Whole Blood Coagulation TimeSubject(s)
Digoxin/pharmacology , Heart/physiology , Adult , Digoxin/administration & dosage , Digoxin/blood , Electrocardiography , Heart/drug effects , Humans , Infusions, Intravenous , Kinetics , MaleABSTRACT
Intraindividually compared to placebo, the possible influence of an oral pretreatment with the cyclooxygenase inhibitor indometacin on serum concentrations and cardiac effects of digoxin, administered by a controlled intravenous infusion, was evaluated in 6 healthy male humans. A significant pharmacokinetic or pharmacodynamic interaction between both drugs could not be found. Indometacin only tended to elevate serum digoxin levels, to strengthen the digoxin induced decrease in the electrocardiogram parameters heart rate and QTc time as well as to reduce the positive inotropic action of digoxin measured by systolic time intervals and PTQ index.