ABSTRACT
Three dibenzocyclooctadiene lignans: deoxyschizandrin (1), gomisin A (2) and schizandrin (3) were isolated from biomass extracts of Schisandra chinensis (Turcz.) Baill. shoot-differentiating callus cultures. The mentioned lignans were not isolated earlier from in vitro cultures of this plant species. This is the first report concerning on isolation of dibenzocyclooctadiene lignans from in vitro cultures of Schisandra chinensis.
Subject(s)
Lignans/chemistry , Schisandra/chemistry , Cells, Cultured , Chromatography, Thin Layer , Cyclooctanes/analysis , Dioxoles/analysis , Lignans/analysis , Magnetic Resonance Spectroscopy , Plant Leaves/chemistry , Plant Leaves/cytology , Plant Shoots/cytology , Polycyclic Compounds/analysisABSTRACT
Contents of schisandrol A and schisandrol B were determined in methanolic extracts of biomass from in vitro cultures of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) using an HPLC method. The biomass was cultured on six variants of Murashige and Skoog (MS) medium containing different concentrations of growth regulators, cytokinin (BAP) and auxin (NAA). The contents of both lignans were different and dependent on the MS medium variant. The maximal contents of schisandrol A (70.54 mg/100 g d.w.) and schisandrol B (86.41 mg/100 g d.w.) were considerable in comparison with plant material: leaves (29.69 and 34.50 mg/100 g d.w.) and fruits (132.39 and 109.40 mg/100 g d.w., respectively). This is the first report on the quantitative analysis of schisandrol A and schisandrol B in Schisandra chinensis in vitro cultures.
Subject(s)
Lignans/analysis , Schisandra/chemistry , Chromatography, High Pressure Liquid , Cyclooctanes/analysis , Dioxoles , Fruit/chemistry , Plant Extracts/analysis , Plant Leaves/chemistry , Plant Shoots/chemistryABSTRACT
The retention behaviour of 15 sulphonamides was investigated by thin-layer reversed-phase ion-pair chromatography (RP-IPC). Optimization of the retention and selectivity of these compounds was carried out by changing the pH, the concentration of the ion-pairing counter-ion and the concentration of the organic solvent in the aqueous mobile phase. The effects of various cationic and anionic pairing reagents in the mobile phase and the stationary phase were investigated. The use of stepwise gradient elution improved the spot shape and the selectivity of the separation.
