ABSTRACT
Identification of cloned cells is necessary for experimentation with them. This paper details a method for the identification of cultured human malignant prostatic epithelial cells derived from metastatic deposits of prostate cancer by localization of a specific rabbit antiserum to human prostatic acid phosphatase in the cells.
Subject(s)
Acid Phosphatase/immunology , Cells, Cultured , Prostatic Neoplasms/enzymology , Antibody Specificity , Clone Cells/enzymology , Epithelium/pathology , Fluorescent Antibody Technique , Humans , Male , Neoplasms, Experimental/enzymology , Prostatic Neoplasms/pathologyABSTRACT
Isolation of the human malignant prostatic epithelial cell in pure monolayer culture was accomplished by clonal growth of single cells derived from metastatic deposits of prostatic carcinoma. Identification of these cells was established by the fact that the acid phosphatase of the prostatic epithelial cell is not inhibited by immersion in 10 per cent neutral formalin up to 24 hours, whereas all other acid phosphatases are destroyed by the formalin.
