ABSTRACT
Examination procedures have to be written for each examination according to the standard requirements. Using CE marked devices, technical inserts can be used, but because of their lack of homogeneity, it could be easier to document their use as a standard procedure. Document control policy applies for those procedures, the content of which could be as provided in this document. Electronic manuals can be used as well.
Subject(s)
Clinical Laboratory Techniques/standards , Quality Assurance, Health Care/legislation & jurisprudence , Clinical Laboratory Techniques/methods , Humans , Information Storage and Retrieval/methods , Quality Assurance, Health Care/standardsABSTRACT
The results obtained using automatic analysers have to be reviewed before release to the laboratory information system (LIMS) for definitive validation by the authorised person. The objective of such a review is to ensure that quality indicators are in agreement with the defined requirements and that the procedures have been carefully fulfilled.
Subject(s)
Clinical Laboratory Information Systems/legislation & jurisprudence , Clinical Laboratory Information Systems/standards , Clinical Laboratory Techniques/standards , Validation Studies as Topic , Humans , Quality Control , Quality Indicators, Health Care , Reproducibility of ResultsSubject(s)
Anesthetics, Local/adverse effects , Hydroxyquinolines/adverse effects , Methemoglobinemia/chemically induced , Oxyquinoline/adverse effects , Anesthetics, Local/administration & dosage , Child, Preschool , Drug Combinations/administration & dosage , Drug Combinations/adverse effects , Female , Humans , Oxyquinoline/administration & dosage , Tooth EruptionABSTRACT
A technique using alternatively dithiothreitol reduction, sonication and pepsin digestions permits the extraction of 75% of the insoluble collagen from human common iliac arteries. The pattern of the collagen types obtained by acrylamide gel electrophoresis is far different from that of the aortic wall: type I averages 44 +/- 14% of the total, type III 48.5 +/- 14% and a supplementary fraction, probably belonging to type V, 9 +/- 5% of the total in females and 6 +/- 2% in males.
Subject(s)
Arteries/analysis , Collagen/isolation & purification , Amino Acids/analysis , Collagen/analysis , Elastin/analysis , Female , Humans , Iliac Artery/analysis , MaleABSTRACT
A fully automatic method permits fractionation in a Biogel P2 column of the hydroxyproline-containing peptides into two fractions. The alkaline hydrolysis and the colorimetric evaluation of the liberated hydroxyproline are also completely automatic and allow calculation of the percentages of the two fractions. The first one, termed F1 fraction, contains the peptides of molecular weight larger than 1500, while the other, termed F2, contains the smaller peptides. The method was used for 223 assays. The F1 fraction is decreased in cases of Paget's bone disease. It is increased in cases of metastatic cancer of bone. Statistical analysis of the data demonstrates that this techique greatly improves the certainty of diagnosis when coupled to the assay of total hydroxyproline. When both total urinary hydroxyproline and the F1 fraction percentage are increased over threshold values of 485 mumol per 24 h and 28.4% respectively, the probability of the presence of a bone metastasis is 100%.
Subject(s)
Hydroxyproline/urine , Peptides/urine , Adolescent , Adult , Autoanalysis/instrumentation , Bone Neoplasms/diagnosis , Bone Neoplasms/secondary , Chemical Fractionation , Child , Female , Humans , Hydrolysis , Middle Aged , Pregnancy , Statistics as TopicABSTRACT
After hydrolysis of the urine by 6 M HCl, 3-hydroxyproline is purified from many interfering substances by 2 steps of chromatography on Dowex 1 X8 resin equilibrated first in the acetate form and secondly in the OH- form. The amino acid is finally evaluated by chromatography on Dowex 50 M82 resin in a Beckman multichrom amino acid analyzer. In 23 normal adult subjects the mean level was 12.5 +/- 3.5 mumol/24 h. In 8 normal children the level was 6.0 +/- 5 mumol/24 h and in 8 teenagers 15.2 +/- 2.85 mumol/24 h. The ratio of 3-hydroxyproline to 4-hydroxyproline seems indicative of a semiological value of this evaluation in cases of basement membrane collagen deterioration.
