T helper 2 inflammatory markers are associated with recurrence in chronic rhinosinusitis with nasal polyps after endoscopic sinus surgery.

BACKGROUND: Despite maximum medical treatment and endoscopic sinus surgery (ESS), chronic rhinosinusitis with nasal polyps (CRSwNP) can require revision surgery. With a growing literature on the diversity of cytokine inflammation patterns in CRSwNP, an endotype-driven approach could lead to the identification of cytokine profiles that predict recurrence. METHODS: A monocentric longitudinal study was carried out until June 2019 following CRSwNP patients who underwent surgery for the first time between December 2010 and January 2012. The biomarker profiles were established on blood and nasal secretions at the time of the first surgery (Interleukin (IL)-5, IgE, IgA, eosinophilic cationic protein (ECP) and eosinophilic- derived neurotoxin (EDN)). Profiles were compared between the patients still controlled by medical treatment and the patients requiring revision surgery during the course of the follow-up period. RESULTS: Among the 48 patients initially enrolled in our study, 8 required revision surgery (16,7%). Clinical features (asthma, allergy, aspirin intolerance, active smoking) and levels of blood markers measured at the time of the first surgery were comparable between the 2 groups of patients. Levels of IL-5, IgE and ECP in nasal secretions were significantly increased in the group of patients needing revision surgery. CONCLUSIONS: Based on simple approach of nasal secretions sampling, we showed that a predominant T helper 2 proteins expression profile can be associated with recurrent CRSwNP after ESS. Initial immunoprofiling in CRSwNP disease may contribute to better predict the therapeutic response to optimal medical and surgical treatment, and help define the role of innovative targeted treatment, beside corticosteroids and ESS.

Complete Genome Sequence of Campylobacter jejuni Strain 12567, a Livestock-Associated Clade Representative.

We report here the complete genome sequence of Campylobacter jejuni strain 12567, a member of a C. jejuni livestock-associated clade that expresses glycoconjugates associated with improved gastrointestinal tract persistence.

Complete Genome Sequences of Three Campylobacter jejuni Phage-Propagating Strains.

Bacteriophage therapy can potentially reduce Campylobacter jejuni numbers in livestock, but it requires a detailed understanding of phage-host interactions. C. jejuni strains readily infected by certain phages are designated as phage-propagating strains. Here, we report the complete genome sequences of three such strains, NCTC 12660, NCTC 12661, and NCTC 12664.

Clinical results of peroneal tendon repair in a retrospective series of 30 patients.

INTRODUCTION: Peroneal tendinopathy is an under-diagnosed pathology, mainly triggered by ankle sprain. Failure of medical treatment often leads to surgery, with modalities adapted to the lesion type. The present study aimed to assess clinical and functional results of tendon repair and to analyze the influence of hindfoot alignment on quality of outcome. The study hypothesis was that hindfoot varus impairs medium-term results. MATERIALS AND METHODS: A retrospective series of 30 patients undergoing peroneal tendon repair or tenodesis was analyzed at a mean 20 months' follow-up. All patients had preoperative ultrasound scan or NMRI. Clinical assessment was based on AOFAS and FAAM scores. Hindfoot alignment was assessed on Méary view; 2 groups were distinguished: valgus (n=11) and varus (n=17). RESULTS: Mean AOFAS score improved, from 74.5±11.2 preoperatively to 86.7±9.4 at follow-up. There was a significant intergroup difference (p=0.0003) in AOFAS at follow-up: valgus, 93/100; varus, 82/100. There was no significant intergroup difference in FAAM score. Time to surgery, lesion type and surgical technique did not influence clinical results. DISCUSSION/CONCLUSION: Clinical results for surgical repair of peroneal tendons were satisfactory and in line with literature reports. However, hindfoot varus was associated with poorer results, raising the possibility of correction by opening wedge calcaneal osteotomy. LEVEL OF EVIDENCE: IV, retrospective case series.

Two cases of distal ulna giant cell tumors treated by extensive resection, prosthetic reconstruction and stabilization using the brachioradialis tendon.

Aggressive giant cell tumors are rare at the distal ulna. We report the results of two patients who were treated surgically using a distal ulna prosthesis stabilized by ligament reconstruction with the brachioradialis tendon. At the maximum follow-up of 18months, the two patients were satisfied. Joint range of motion and functional scores were improved. X-rays showed no recurrence or subluxation; however gradual impingement of the ulnar notch of radius by the prosthesis was visible. Our technique seems to be a viable alternative when compared with the treatments described in other published case reports.

Coadministration of the Campylobacter jejuni N-Glycan-Based Vaccine with Probiotics Improves Vaccine Performance in Broiler Chickens.

Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chickens, but only a fraction of birds respond to vaccination. Optimization of the vaccine for commercial broiler chickens has great potential to prevent the entry of the pathogen into the food chain. Here, we tested the same vaccination approach in broiler chickens and observed similar efficacies in pathogen load reduction, stimulation of the host IgY response, the lack of C. jejuni resistance development, uniformity in microbial gut composition, and the bimodal response to treatment. Gut microbiota analysis of leghorn and broiler vaccine responders identified one member of Clostridiales cluster XIVa, Anaerosporobacter mobilis, that was significantly more abundant in responder birds. In broiler chickens, coadministration of the live vaccine with A. mobilis or Lactobacillus reuteri, a commonly used probiotic, resulted in increased vaccine efficacy, antibody responses, and weight gain. To investigate whether the responder-nonresponder effect was due to the selection of a C. jejuni "supercolonizer mutant" with altered phase-variable genes, we analyzed all poly(G)-containing loci of the input strain compared to nonresponder colony isolates and found no evidence of phase state selection. However, untargeted nuclear magnetic resonance (NMR)-based metabolomics identified a potential biomarker negatively correlated with C. jejuni colonization levels that is possibly linked to increased microbial diversity in this subgroup. The comprehensive methods used to examine the bimodality of the vaccine response provide several opportunities to improve the C. jejuni vaccine and the efficacy of any vaccination strategy.IMPORTANCECampylobacter jejuni is a common cause of human diarrheal disease worldwide and is listed by the World Health Organization as a high-priority pathogen. C. jejuni infection typically occurs through the ingestion of contaminated chicken meat, so many efforts are targeted at reducing C. jejuni levels at the source. We previously developed a vaccine that reduces C. jejuni levels in egg-laying chickens. In this study, we improved vaccine performance in meat birds by supplementing the vaccine with probiotics. In addition, we demonstrated that C. jejuni colonization levels in chickens are negatively correlated with the abundance of clostridia, another group of common gut microbes. We describe new methods for vaccine optimization that will assist in improving the C. jejuni vaccine and other vaccines under development.

Additionality and permanence standards in California's Forest Offset Protocol: A review of project and program level implications.

A key component of California's cap-and-trade program is the use of carbon offsets as compliance instruments for reducing statewide GHG emissions. Under this program, offsets are tradable credits representing real, verifiable, quantifiable, enforceable, permanent, and additional reductions or removals of GHG emissions. This paper focuses on the permanence and additionality standards for offset credits as defined and operationalized in California's Compliance Offset Protocol for U.S. Forest Projects. Drawing on a review of the protocol, interviews, current offset projects, and existing literature, we discuss how additionality and permanence standards relate to project participation and overall program effectiveness. Specifically, we provide an overview of offset credits as compliance instruments in California's cap-and-trade program, the timeline for a forest offset project, and the factors shaping participation in offset projects. We then discuss the implications of permanence and additionality at both the project and program levels. Largely consistent with previous work, we find that stringent standards for permanent and additional project activities can present barriers to participation, but also, that there may be a trade-off between project quality and quantity (i.e. levels of participation) when considering overall program effectiveness. We summarize what this implies for California's forest offset program and provide suggestions for improvements in light of potential program diffusion and policy learning.

Results and limitations of humeral head resurfacing: 105 cases at a mean follow-up of 5 years.

BACKGROUND: The objective of this study was to assess clinical and computed-tomography (CT) outcomes at least 2 years after humeral head resurfacing to treat concentric gleno-humeral osteoarthritis. HYPOTHESIS: Humeral head resurfacing provides similar outcomes to those achieved with stemmed humeral head implants. MATERIALS AND METHODS: This single-centre retrospective study included 40 Copeland™ and 65 Aequalis™ humeral resurfacing heads implanted between 2004 and 2012. Mean patient age at diagnosis was 64 years. The diagnoses were osteoarthritis with an intact (68%) or torn (21%) rotator cuff, avascular necrosis (5%), osteoarthritis complicating chronic instability (3%), post-traumatic osteoarthritis (2%), and chronic inflammatory joint disease (1%). Validated clinical scores, radiographs, and CT before surgery and at last follow-up were compared. RESULTS: During the mean follow-up of 56 months, complications occurred in 24 implants. Revision surgery with reverse shoulder replacement was required in 18 cases, after a mean of 43.6 months, to treat glenoid wear or a rotator cuff tear. At last follow-up, for the implants that did not require revision surgery, the mean Constant score was 64/100. The implants had a mean varus of 5° and mean retroversion of -13.3°. The mean increase in glenoid cavity depth was 2.4mm. Mean increases in medial and lateral humeral offset were 1.9mm and 2.7mm, respectively. Pre-operative factors significantly associated with failure were rotator cuff tear (P=0.017) and glenoid erosion (P=0.001). DISCUSSION: We found a high failure rate related to glenoid wear or progressive rotator-cuff impairment, although CT showed no evidence of implant malposition or overstuffing. Previous studies of stemmed humeral head implants showed better outcomes. Given the low medium-term prosthesis survival rate, we now reserve humeral head resurfacing for concentric osteoarthritis without glenoid erosions or rotator cuff damage. LEVEL OF EVIDENCE: IV, retrospective study.

Local extension staging of sinonasal tumours: retrospective comparison between CT/MRI assessment and pathological findings.

OBJECTIVES: To assess the reliability of clinical staging with CT and MRI in sinonasal cancers. METHODS: We conducted a retrospective review of patients who underwent surgery for a sinonasal cancer. The 7th edition of the TNM classification was used to establish tumour staging. Standardised preoperative CT/MRI staging was compared with the pathological staging based on specimens obtained during surgery from each subsite within and around the tumour. RESULTS: We analysed data from 68 patients between January 2010 and December 2014. A comparison of cT and pT stages was established for 49 naso-ethmoidal and 16 maxillary tumours. Clinical staging for naso-ethmoidal cT1 and maxillary cT2 was consistent with pathological results. Clinical staging for naso-ethmoidal cT2, cT3 and cT4b was overstated in comparison with pT findings. The positive predictive value of imaging was <65% for the lamina papyracea, the cribriform plate, the dura, and the frontal and sphenoid sinuses. Sensitivity was over 75% for each anatomical site except for the cribriform plate (73.3%) and the sphenoid sinus (57.2%). CONCLUSION: Systematic pathologic analysis of the anatomical areas around the sinonasal cancer has to be applied in further studies to improve our therapeutic management. CT/MRI mapping cannot replace accurate assessment of tumour extension during surgery.

Prospective observational study of midtarsal joint sprain: Epidemiological and ultrasonographic analysis.

INTRODUCTION: Foot and ankle injuries (FAI) are very common, with about 6000 cases per day in France. Unlike lateral ankle sprain (LAS), the diagnosis of midtarsal joint sprain (MJS, also known as Chopart's joint sprain) is not widely known. This prospective study aims to detail the epidemiology of MJS and compare it to LAS. PATIENTS AND METHOD: The study was conducted within our institution over a period of 16 months. Patients with clinical signs predictive of MJS without radiographic bone lesion underwent ultrasound assessment. MJS was diagnosed in case of at least 1 lesion of the dorsal midtarsal joint ligaments. RESULTS: A total of 2412 patients consulted for FAI; 188 had clinical and radiographic criteria for ultrasound examination. Eighty-two cases of MJS were diagnosed (3.4% of FAIs). Sports injuries were more frequent in MJS (P=0.04), and mechanisms more varied than in LAS, with inversion injury in 75% of cases and plantar hyperflexion in 22%. Sprain was severe in 70% of cases, with complete ligament tear. Clinical and ultrasound analyses correlated in only 40% of cases of MJS, versus 98% for LAS. CONCLUSION: MJS is frequent, difficult to diagnose clinically, and often severe. Clinical presentation and injury mechanisms differ from ankle sprain. Ultrasound seems to be an indispensable tool in diagnosis.

Correlation between primary flat foot and lower extremity rotational misalignment in adults.

PURPOSE: The goal of this study was to identify rotational abnormalities of the lower limb in adult patients with primary symptomatic flatfoot. MATERIALS AND METHODS: From September 2009 to May 2012, 24 patients (12 women, 12 men; mean age: 40 years) were prospectively included in the study. Each patient underwent radiographs of the flat foot and weight-bearing upright EOS® examination. Three-dimensional reconstructions of the lower extremities were performed with derived measurements (length, hip and knee parameters, rotations). A total of 31 symptomatic primary flat feet (bilateral flat foot, n=7 patients; unilateral flat foot, n=17 patients) were studied and compared to 30 control subjects matched for age and gender. A comparison between the two groups was made with the Student t-test. RESULTS: No significant differences were found between patients and control subjects on the coronal and sagittal planes. Similarly, no significant differences were observed between the 2 groups for rotation of the lower limbs (femoral torsion, tibial torsion, tibiofemoral rotation). CONCLUSION: There are no rotational abnormalities of the lower extremities in adult patients with primary symptomatic flat foot.

Chondrosarcoma of the femur in Mazabraud's syndrome: A first case study.

Malignant transformation of fibrous dysplasia (FD) in long bones is rare (less than 1% of cases) and occurs in the form of osteosarcomas. They can occur on FD associated with multiple myxomas, which corresponds to Mazabraud syndrome. To our knowledge, only six cases of FD malignant transformation into osteosarcoma in the context of Mazabraud syndrome have been described. Here, we describe the first case of malignant transformation of femur FD into chondrosarcoma in a 51-year-old female patient. The patient had significant changes in the size and number of myxomas around the femur before the malignant transformation into chondrosarcoma. She underwent complete femur replacement with a favourable result after 4 years' follow-up. Close surveillance is strongly recommended in this syndrome, particularly when the number and size of myxomas increase.

Olfactory cleft opacity and CT score are predictive factors of smell recovery after surgery in nasal polyposis.

OBJECTIVES: To assess subjective improvement of olfactory function following endoscopic sinus surgery (ESS) in chronic rhinosinusitis associated with nasal polyps (CRSwNP) and to analyse factors of recovery with the European Test of Olfactory Capabilities (ETOC). METHODS: We carried out a prospective study of 30 patients with CRSwNP from November 2011 to April 2013. The ETOC was filled the day before surgery and in the short term follow-up. Sixteen suprathreshold odorants with a detection task and a forced choice verbal identification task were tested. RESULTS: The mean composite score (MCS) improved at 3 and 6 months. The preoperative MCS was correlated to the Lund-Mackay score and to the olfactory cleft opacification on preoperative computed tomography (CT) scan. Multivariate linear regression modelling of patients with preoperative anosmia showed that the olfactory recovery at 3 months was predicted by the preoperative Lund-Mackay score and the age, and at 6 months by the preoperative Lund-Mackay score. CONCLUSION: With a convenient psychophysical test, we showed that olfactory cleft opacification and CT scan score could be predictive factors of olfaction disorder severity and improvement after ESS in CRSwNP. These results need to be strengthened in the long term with a larger panel of patients.

Percutaneous CT-guided radiofrequency thermocoagulation in the treatment of osteoid osteoma: a 87 patient series.

INTRODUCTION: Osteoid osteoma is a painful, benign bone tumor that mainly affects young people. Thermocoagulation is one of the recommended percutaneous treatment methods. This study sought to assess its efficacy and identify risk factors for osteoma recurrence. METHODS: Results were analyzed retrospectively for a group of 87 patients treated by thermocoagulation between 2002 and 2011. The recurrence rate was calculated and analyzed relative to patient and tumor characteristics. The treatment efficacy was determined and methods to prevent complications were analyzed. RESULTS: The mean follow-up time was 34 months. The average patient age was 23 years. There were seven complications including three patients with delayed wound healing, mainly at tibial sites. The recurrence rate was 10.4%. The success rate for first-line treatment was 89.6% and it was 97.5% for second-line treatment. Analysis of patient characteristics and tumor locations revealed no risk factors for recurrence. CONCLUSION: Percutaneous thermocoagulation is a reliable and effective technique that provides fast, long-lasting pain relief. However recurrence can occur even after the nidus is completely resected. These recurrences can be effectively managed by repeat treatment. Recent technical improvements have reduced the risk of thermocoagulation-related complications. LEVEL OF EVIDENCE: IV.

Anatomic and CT scan assessment of Teres Minor: a new index of trophicity.

INTRODUCTION: The present study reports the development of a CT assessment protocol for Teres Minor (TM) trophicity. HYPOTHESIS: Quantitative reproductible Terres Minor assessment on CT estimates the influence of muscle trophicity on the clinical and radiological results of palliative treatment of irreparable rotator cuff tear. MATERIALS AND METHOD: An anatomic study of 30 cadaveric shoulders confirmed a constant anatomic relation between Terres Minor and the inferior pole of the glenoid cavity. This landmark was used to develop a novel CT assessment of TM trophicity. RESULTS: The CT assessment showed excellent inter- and intra-observer reproductibility. The protocol defines a trophicity index, T2/G (T2 being TM thickness on axial CT slice, and G the maximum glenoid cavity thickness on axial slice), enabling reproductible TM analysis on preoperative arthro-CT. CONCLUSION: The study validated the CT protocol, allowing application in pre- and postoperative assessment of irreparable rotator cuff tear. LEVEL OF EVIDENCE: Level IV. Retrospective study.

[Management of the avulsion of the flexor pollicis longus and flexor indicis profundus tendons by tendon transfer. Case report]. / Prise en charge d'une avulsion du flexor pollicis longus et flexor profundus indicis par transferts tendineux en urgence. À propos d'un cas.

Tendon injuries are common in hand wounds and their functional consequences are not negligible. In emergency, tendon repairs techniques are well codified to restore function. These tendon repairs can be made in one session, in two sessions, or even use tendon transfers. Tendon transfers are not usual in the emergency but more common in the treatment of sequelae or failure of primary repair. We report one case of transfer to restore thumb flexion in emergency.

Reproducibility and reliability of subscapularis tendon assessment using CT-arthrography.

BACKGROUND: Computed tomography (CT) coupled with arthrography remains the reference standard for the preoperative evaluation of rotator cuff tears. The objectives of this study were to evaluate intra-observer and inter-observer reproducibilities of CT-arthrographic assessment of the subscapularis tendon and to assess the validity and reliability of this investigation. HYPOTHESIS: CT-arthrography is reliable and reproducible for the preoperative characterisation of subscapularis tendon lesions. MATERIAL AND METHODS: We retrospectively reviewed 67 shoulders with rotator cuff tears in one or more tendons managed by arthroscopy, both to confirm the diagnosis and to allow therapeutic interventions. Each of the 67 preoperative CT-arthrograms was evaluated by three readers, of whom the first two evaluated the images twice at an interval of 30 days. The following were recorded at each reading: partial- or full-thickness tear in one or more rotator cuff tendons, intra-tendinous delamination, tendon stump retraction, and fatty degeneration of the muscles. The position of the long head of biceps tendon was assessed. A statistical analysis was performed using Fleiss' method to compute intra-observer and inter-observer variabilities in CT-arthrography assessment of the subscapularis tendon status. Validity of this assessment was measured by computing the concordance coefficients between CT-arthrography and arthroscopy. RESULTS: Specificity of CT-arthrography was satisfactory for assessing the subscapularis tendon. Sensitivity was low. Significant inter-observer and intra-observer variabilities were documented. The concordance coefficients between CT-arthrography and arthroscopy indicated that major differences were common with all three readers. When assessing the subscapularis tendon by CT-arthrography, all readers experienced difficulties in distinguishing intact tendons, delaminated tendons, and tendons with tears confined to the upper third. DISCUSSION: Although CT-arthrography remains the reference standard for the preoperative investigation of rotator cuff tears, significant variability occurs in assessing the continuity of the subscapularis tendon. Reliability of this assessment is not optimal, as shown by our evaluation of concordance with arthroscopy. Although our data should be interpreted in the light of the investigation and measurement biases present in our study, they suggest that CT-arthrography may fail to provide a valid and reproducible assessment of the subscapularis tendon. The development of magnetic resonance (MR) imaging and MR-arthrography will probably improve the preoperative evaluation of subscapularis tears in the near future. LEVEL OF EVIDENCE: Level III; diagnostic value study.

Geometrical analysis results of 42 resurfacing shoulder prostheses: A CT scan study.

BACKGROUND: Shoulder resurfacing arthroplasty was introduced in Scandinavia in the early 1980s then developed by SA Copeland. HYPOTHESIS: Resurfacing prostheses restore the normal anatomy of the proximal humerus. Here, our objective was to evaluate humeral resurfacing prosthesis position on radiographs and computed tomography (CT) images. MATERIALS AND METHODS: We retrospectively reviewed 42 consecutive cases seen at a single centre between 2004 and 2009. Mean patient age was 65 years. CT was performed routinely before prosthesis implantation and at re-evaluation. The Copeland Mark III(®) (Biomet France SARL, 26903 Valence, France) implant was used in 32 cases and the Aequalis Resurfacing Head(®) (Tornier France, 38334 Saint-Ismier, France) in 10 cases. The post-implantation CT images were used to measure the angle of inclination, medial humeral offset, lateral glenohumeral offset, and version of the implant. RESULTS: Mean follow-up was 18 months. Compared to baseline, no significant changes were found at re-evaluation for the angle of inclination or lateral glenohumeral offset. In contrast, medial humeral offset increased by 3.47mm, and excessive anteversion of 4.23° compared to the bicondylar line was noted. DISCUSSION: Humeral head resurfacing prostheses restore the overall anatomy of the proximal humeral head. Our CT scan evaluation protocol seems reproducible and enables an evaluation of implant geometry. In our experience, resurfacing arthroplasty restored the native humeral offset. Inadequate retroversion was noted and was probably related to insufficient exposure during surgery. LEVEL OF EVIDENCE: Level IV, retrospective study.

Functional performance after hip resurfacing or total hip replacement: a comparative assessment with non-operated subjects.

INTRODUCTION: Several studies reported better clinical results with total hip resurfacing than with conventional total hip replacement, including in young patients, but without comparative stabilometry assessment. HYPOTHESIS: Resurfacing arthroplasty provides better functional performance than conventional total hip replacement. OBJECTIVES: To test the above hypothesis in a stabilometry study comparing balance and functional performance in patients with total hip resurfacing or conventional total hip replacement and healthy controls. MATERIALS AND METHODS: Results were analyzed on three cohorts of 20 patients: healthy control subjects, with unilateral total hip replacement or unilateral total hip resurfacing. The 40 operated patients were comparable in gender, age, weight (body-mass index), date of operation and clinical results. The 20 control subjects were younger and served as reference. Balance analysis employed a force platform commonly used in stabilometry, standardizing both leg or single leg stance balance analysis. The software interpreted individual balance by measuring plantar pressure center variation during the analysis so as to contour an individual both leg or single leg area of balance (statokinesigram, in mm(2)). RESULTS: Balance analysis on both leg found comparable results in the control and resurfacing groups. The weight-bearing statokinesigraphic both leg balance area was greater in the hip replacement than in either of the other two groups (p<0.05), and five times greater than in the resurfacing group (p<0.05). The single leg weight-bearing balance results were significantly better in the resurfacing group, with a statokinesigraphic balance area half that of the hip replacement group, whether on the operated or the non-operated side (p<0.001). DISCUSSION AND CONCLUSION: The present functional performance results confirm the advantage of resurfacing over conventional hip replacement, and help explain the excellent radioclinical results reported for total hip resurfacing. LEVEL OF EVIDENCE: III, comparative case-control study.