Delayed-onset muscle soreness in human masticatory muscles increases inhibitory jaw reflex responses.

The effects of masticatory muscles' overloading on jaw-motor control are not yet fully clarified. Therefore, it was tested whether eccentric and concentric exercises of the human masticatory muscles would influence inhibitory jaw reflex responses. Eleven participants (6 males, 5 females) performed 6, 5-minutes bouts of eccentric-concentric contractions. Before, immediately after, 24 hours, 48 hours and 1 week afterwards, visual analogue scale (VAS) scores for jaw muscle fatigue and pain, maximum voluntary bite force (MVBF) and inhibitory jaw reflexes were recorded. Reflex data were analysed with the cumulative sum control chart error box method. Immediate and delayed masticatory muscle fatigue and pain were provoked. Further, 24 hours after the exercises, MVBF tended to decrease (P = .056), suggesting that delayed-onset muscle soreness (DOMS) was provoked in the masticatory muscles. In addition, the inhibitory jaw reflex showed a delayed increase in size 24 hours after the exercise (P < .05). In conclusion, DOMS provoked in the masticatory muscles alters jaw motor control by inducing a delayed increase in the size of the inhibitory jaw reflex.

Tonic activity of the human temporalis muscle at mandibular rest position.

OBJECTIVE: There are two theories on the control of the human mandibular rest position; the visco-elastic theory and the muscular theory. In this study, we have searched for evidence for the muscular theory. DESIGN: We have investigated the activity of the anterior temporalis muscle during various positions of the mandible using intramuscular electrodes. RESULTS: In nine out of ten subjects tonic activity in the anterior temporalis muscle during the mandibular rest position was observed. Most of these units ceased firing under the jaw dropped condition of the mandible. CONCLUSIONS: Current findings support the muscular theory since single motor unit activity during the rest position of the mandible is observed in the anterior temporalis. We do not however have any evidence against the visco-elastic theory which may contribute in part to maintain the rest position of the mandible.

Jaw tremor as a physiological biomarker of bruxism.

OBJECTIVE: To determine if sleep bruxism is associated with abnormal physiological tremor of the jaw during a visually-guided bite force control task. METHODS: Healthy participants and patients with sleep bruxism were given visual feedback of their bite force and asked to trace triangular target trajectories (duration=20s, peak force <35% maximum voluntary force). Bite force control was quantified in terms of the power spectra of force fluctuations, masseter EMG activity, and force-to-EMG coherence. RESULTS: Patients had greater jaw force tremor at ∼8 Hz relative to controls, along with increased masseter EMG activity and force-to-EMG coherence in the same frequency range. Patients also showed lower force-to-EMG coherence at low frequencies (<3 Hz), but greater coherence at high frequencies (20-40 Hz). Finally, patients had greater 6-10 Hz force tremor during periods of descending vs. ascending force, while controls showed no difference in tremor with respect to force dynamics. CONCLUSION: Patients with bruxism have abnormal jaw tremor when engaged in a visually-guided bite force task. SIGNIFICANCE: Measurement of jaw tremor may aid in the detection/evaluation of bruxism. In light of previous literature, our results also suggest that bruxism is marked by abnormal or mishandled peripheral feedback from the teeth.

Reflex responses of human masseter motor units to mechanical stimulation of the teeth.

Our aim was to investigate the jaw reflexes using both the probability- and the discharge rate-based analysis methods. Twelve consenting volunteer subjects participated in this study. Subjects bit gently on bite bars that carried the impression of their teeth. Surface and intramuscular electrical activity of the masseter was recorded. With the help of audio feedback from one motor unit, each subject bit to discharge the unit at a fixed rate. While the subject continuously activated the selected motor unit, 4-N stimuli were delivered to the upper right central incisor either at a rapid or a slow rate. For each trial, ≥300 stimuli were delivered, and, once a trial was completed, local anesthetic block was applied around the stimulated tooth, and the experiment was repeated. While preceding local anesthesia, the rapid-rate stimuli ("tap") induced substantial inhibitory reflex responses; during local anesthetic block, the same stimulus induced excitatory and inhibitory reflex responses. Slow-rate stimuli ("push"), on the other hand, usually generated a combination of inhibitory and excitatory responses that disappeared completely during the local anesthetic block. This study discovered that the strength of the inhibitory reflex response to a tooth-tap stimulus was much larger than previously reported. This study also found that whereas the probability-based analyses were better for illustrating the existence and latency of small earlier responses, the discharge rate-based method was better for indicating the duration of earlier responses and the existence, sign, and duration of later responses.

Estimates of EPSP amplitude based on changes in motoneuron discharge rate and probability.

When motor units are discharging tonically, transient excitatory synaptic inputs produce an increase in the probability of spike occurrence and also increase the instantaneous discharge rate. Several researchers have proposed that these induced changes in discharge rate and probability can be used to estimate the amplitude of the underlying excitatory post-synaptic potential (EPSP). We tested two different methods of estimating EPSP amplitude by comparing the amplitude of simulated EPSPs with their effects on the discharge of rat hypoglossal motoneurons recorded in an in vitro brainstem slice preparation. The first estimation method (simplified-trajectory method) is based on the assumptions that the membrane potential trajectory between spikes can be approximated by a 10 mV post-spike hyperpolarization followed by a linear rise to the next spike and that EPSPs sum linearly with this trajectory. We hypothesized that this estimation method would not be accurate due to interspike variations in membrane conductance and firing threshold that are not included in the model and that an alternative method based on estimating the effective distance to threshold would provide more accurate estimates of EPSP amplitude. This second method (distance-to-threshold method) uses interspike interval statistics to estimate the effective distance to threshold throughout the interspike interval and incorporates this distance-to-threshold trajectory into a threshold-crossing model. We found that the first method systematically overestimated the amplitude of small (<5 mV) EPSPs and underestimated the amplitude of large (>5 mV EPSPs). For large EPSPs, the degree of underestimation increased with increasing background discharge rate. Estimates based on the second method were more accurate for small EPSPs than those based on the first model, but estimation errors were still large for large EPSPs. These errors were likely due to two factors: (1) the distance to threshold can only be directly estimated over a limited portion of the interspike interval and (2) the distance to threshold can be affected by the EPSP itself. Both methods provide the most accurate EPSP estimates for EPSP amplitudes less than 5 mV and moderate background discharge rates (~15 imp/s).

Threshold for detection of incisal forces is increased by jaw movement.

Current knowledge regarding the sensitivity of the teeth to forces is based on psychophysical experiments that measured touch detection thresholds under static jaw conditions. It is not known whether jaw movements alter the perception of forces applied to the teeth, but, based on limb movement studies, it is hypothesized that the perception of mechanoreceptor outputs will be downwardly modulated by jaw movements. We predicted that, compared with static jaw conditions, rhythmic jaw movements would be associated with significantly higher psychophysical thresholds for the detection of incisally applied forces. In eight participants, mechanical pulses were delivered to an incisor during static jaw holding or during cyclic jaw opening and closing. Analogous to findings in human limbs, the psychophysical salience of periodontal mechanoreceptor feedback was downwardly modulated by physiologically relevant movements; detection thresholds for mechanical pulses applied to a central incisor were significantly higher during jaw-closing movements than during static jaw positioning.

Modulation of masseteric reflexes by simulated mastication.

It is well-known that limb muscle reflexes are modulated during human movements. However, little is known about the existence of equivalent masticatory muscle reflex modulation. We hypothesized that masticatory reflexes would be modulated during chewing so that smooth masticatory movements occur. To examine this hypothesis, we studied the modulation of inhibitory reflexes evoked by periodontal mechanoreceptor activation and of excitatory reflexes evoked by muscle spindle activation during simulated mastication. In 28 participants, 1- and 2-N mechanical taps were delivered to the incisor. Reflex responses to these taps were examined in the average masseteric electromyogram. To differentiate between periodontal mechanoreceptor- and muscle-spindle-mediated reflex components, we performed experiments prior to, and in the presence of, periodontal anesthesia. Both periodontal mechanoreceptor and muscle spindle reflexes were reduced during simulated masticatory movements.

Modulation of human exteroceptive jaw reflexes during simulated mastication.

OBJECTIVE: To investigate changes in synaptic input from lower lip afferents to human jaw muscle motoneurons during simulated mastication. METHODS: The lower lip of 14 subjects was stimulated electrically under static and dynamic conditions. In the static condition, subjects bit at mid-open position and received stimuli while keeping the masseteric excitation level at 20%, 40%, 60%, 80%, or 100% of the maximum EMG (generated during simulated chewing). In the dynamic condition, the subjects 'masticated' at their habitual chewing pace, and stimuli were delivered whenever the jaw crossed a predetermined gape. In both conditions, mildly (scores of 2-3 on a 0-10 rating scale) and moderately (scores of 5-6) painful stimulus intensities were used. RESULTS: Under static conditions, there was no modulation of the inhibitory masseteric reflexes with the level of the background level of excitation used in these experiments. However, under dynamic conditions there were significant strength modulations with gape that differed between mildly and moderately painful stimuli. CONCLUSIONS: Reflexes in response to mildly painful stimuli were 'gated' during simulated mastication: as the teeth moved closer toward occlusion, the inhibitory response was progressively reduced. Conversely, responses to moderately painful stimuli became stronger as the teeth moved closer toward occlusion. SIGNIFICANCE: The modulation described allows smooth mastication to occur as it gates out mildly painful signals while responding strongly when the signal indicates potential or actual damage closer to occlusion.

Periodontal-masseteric reflexes decrease with tooth pre-load.

The responses of incisal periodontal mechanoreceptors to increasing mechanical stimulation are known to follow a hyperbolic-saturating course. The implications of these properties for the reflexive control of bite-force have not been examined directly. In line with the above mentioned receptor characteristics, we hypothesized that the periodontal-masseteric reflex will reduce as a function of increasing incisal pre-load. In 10 participants, a central incisor was repeatedly tapped (0.4 N). We measured the modulation by pre-load (0.2-2.0 N) of the reflex frequency-response at and between 3 and 20 Hz. The entrainment of the reflex increased with frequency up to 20 Hz and diminished with increasing pre-load. Importantly, the hyperbolic relationship shown here between the periodontal-masseteric reflex and tooth pre-load agreed with the load/response relationships predicted by single-receptor and tooth movement studies. This study demonstrated that periodontal mechano-receptors are able to contribute to the ongoing control of only small bite-forces.

Standardization of H-reflex analyses.

Variability in the H-reflex can make it difficult to identify significant changes using traditional pooled analysis techniques. This study was undertaken to introduce a normalisation approach to calculate both the relative size and the relative stimulus intensity required to elicit the H-reflex response so that comparisons can be made not only with results obtained during different experimental session but also between different subjects. This normalisation process fits the size of the measured M-responses and H-reflexes over the entire stimulus range with model curves to better facilitate the calculation of important parameters. This approach allows normalisation of not only the size of the response but also the relative stimulus intensity required to elicit the response. This eases the comparison of the reflex responses under various situations, and is capable of bringing out any genuine differences in the reflex in a reliable manner not previously possible. This study illustrates that comparison of the reflex between days is problematic, even in the same subject, as both the reflex size and the relative stimulus intensity required to obtain this reflex changed in all subjects. We suggest that H-reflex studies need to use normalisation not only for size of the reflex but also for the stimulus intensity, and also that all experiments for a single subject should be performed in the same session or during the same day using some level of background muscle activity in the muscle concerned as the variability of the muscle at rest was found to be larger.

Triceps surae stretch and voluntary contraction alters maximal M-wave magnitude.

UNLABELLED: Reliability of the motor response (M-wave) is fundamental in many reflex studies; however it has recently been shown to change during some investigations. The aim of this investigation was to determine if triceps surae stretch and voluntary contraction, or recording and analysis techniques, affect the maximal M-wave magnitude. The maximal M-wave was investigated in human gastrocnemius and soleus during different foot positions and during triceps surae contraction. Both bipolar and monopolar-recoding methods, and area and peak-to-peak (PTP) amplitude analysis methods were used. RESULTS: Maximal M-wave magnitude changed significantly between test muscle conditions, and is largest during dorsiflexion, probably due to changes in muscle bulk and recording electrode relationship. The maximal M-wave was up to 88% smaller when recorded by bipolar electrodes compared to monopolar electrodes, which is discussed in relation to signal cancellation. Area analysis provided more significant differences in M-wave magnitude between test muscle conditions than did PTP amplitude analysis, and the maximal M-wave shape changed significantly between test muscle conditions. This study suggests that maximal M-wave magnitude can vary depending on muscle condition, it highlights the importance of using correct recording and analysis techniques, and questions the reliability of using M-wave magnitude to monitor the relationship between the nerves and stimulating electrodes.

Is the human masticatory system devoid of recurrent inhibition?

The aim of the present study was to investigate the existence or otherwise of a functional recurrent inhibitory system (Renshaw cell system) in the motoneurons that innervate human masticatory muscles. In a previous study, L: -acetylcarnitine (L: -Ac), a substance known to potentiate recurrent inhibition in humans was found to alter, in a specific way, the discharge variability, and the synchronous activity of motor units depending on the presence or absence of recurrent inhibition in the corresponding motoneuron pool. Using a similar paradigm, we have recorded the tonic discharge activity of motor unit pairs from the masseter muscle during voluntary isometric contraction while subjects were undergoing continuous intravenous saline (SAL, NaCl 0.9%) perfusion. Following a brief baseline-recording period, the subjects were given a test injection of either L: -Ac or isotonic saline (SAL) in a double blind manner. The variability, synchronization, and coherence between the motor unit discharges were analysed during three successive periods: pre-injection, during injection, and post-injection, each lasting 2-3 min. Neither L: -Ac nor SAL injection induced a significant change in the inter-spike interval (ISI) or the coefficient of variation of the ISIs in the motor units tested. There were also no significant changes in the pattern of synchronous activity or in the coherence, which reflects the common frequency content of the unit discharges. Reminiscent of what had been observed previously with motoneurons without recurrent inhibition in the Abductor Digitorum Minimi muscle, the lack of effects of L: -Ac injection on the firing behaviour of masseter motoneurons may suggest that classical Renshaw cell inhibition is lacking in this motoneuron pool.

A new method to estimate signal cancellation in the human maximal M-wave.

A new method is introduced that estimates EMG signal cancellation in surface recorded investigations. Its usefulness is demonstrated when determining changes in the maximal motor response (M-wave) magnitude during rest and voluntary contraction. The accuracy of recording and analysis methods and the reliability of the maximal M-wave were assessed in the human gastrocnemius and soleus. The maximal M-wave was recorded by bipolar surface electrodes placed 2 cm, 3 cm and 4 cm apart, and by monopolar (one active and one indifferent reference) surface electrodes. Up to 85% of the maximal M-wave was lost due to signal cancellation during bipolar recording. The maximal M-wave magnitude decreased consistently and significantly during triceps surae contraction compared to rest when recorded by monopolar electrodes, but not when recorded by bipolar electrodes. Area and peak-to-peak (PTP) amplitude analysis methods provided similar results when determining the magnitude of the maximal M-wave. This provides evidence that monopolar recording is superior to bipolar recording as it removes the signal cancellation error and allows the genuine changes in maximal M-wave magnitude to be observed.

Black box revisited: a technique for estimating postsynaptic potentials in neurons.

Our understanding of the operation of the brain depends on knowledge of its wiring. Currently, the wiring of the human brain is estimated by counting the number of neuron discharges that occur at specific times following a stimulus. There is now strong evidence that this approach generates significant errors. Recently, the accuracy of this 'count' method has been compared directly with an alternative 'rate' method in rat brain slices. The results confirmed that the count method generates significant errors that are minimized by the rate method, because the rate of discharge of a neuron accurately displays its excitability at the time of discharge. Therefore, it is now crucial that the rate method be used to reassess previous estimates of the characteristics of wiring in the brain.

A device for investigating neuromuscular control in the human masticatory system.

A new apparatus has been developed to study the control of mastication in humans. The subject places his/her teeth on fixed upper and mobile lower bite plates; the device then enables opening and closing movements of the lower jaw against a controlled resistance. It is also possible to vary the number of teeth in contact with the device during an experiment from the entire dental arcade to a single tooth. The specially designed lower bite plate is dynamic and allows for both rotation and translation of the lower jaw during movement, thus, permitting the natural curvilinear trajectory of the jaw. The lower bite plate can follow chewing initiated by the subject without resisting the movement ('no force' mode) via a dedicated microprocessor controlled compensation mechanism. Another function of the device is to inject a constant predetermined load onto the lower bite plate so that the subject 'chews' against a fixed resistance simulating rapidly yielding food bolus ('fixed force' mode). The device can be programmed to increase or decrease the force during the closing or opening phase of chewing by feeding the position information into the force compensation system so both position and force change in parallel, hence, simulating a bite onto a non-yielding, or sticky, food bolus ('normal chewing' mode). By use of a jaw position compensation mechanism, the device can actively move the lower jaw, following any imposed position pattern ('position controlled' mode). The chewing simulator also has a mode that holds the position at a fixed level and allows the force to change ('position hold' mode). Furthermore, the device can inject additional rapid or slow forces or displacements onto the lower bite plate in order to elicit reflexes so that the response of jaw muscles to such stimuli can be examined at various jaw positions, force levels, phases of motion and velocities. The different modes of the apparatus can be used to study the operation and feedback control of human mastication; in particular whether modulations in jaw muscle activity and reflexes are due to changes in force, velocity, position, chewing cycle phase or a combination of these factors.

Muscle spindle feedback differs between the soleus and gastrocnemius in humans.

UNLABELLED: The Hoffmann (H) reflex and motor (M) response were studied in soleus and gastrocnemius during voluntary contraction in eight male volunteers. AIMS: To determine if the strength of spindle input to the muscles is the same. To assess if the M response size changes during contraction. RESULTS: The size of the maximum M response (M max) changed during contraction in each subject. Hence, all H reflex measurements were normalized to the M max at each level of contraction for each subject. The largest H/M max was bigger in soleus than gastrocnemius at every contraction level. The overall largest H/M max for soleus (97%) and gastrocnemius (55%) were achieved at 40 and 100% maximum voluntary contraction (MVC), respectively. CONCLUSION: Soleus receives greater spindle feedback than the gastrocnemius both at rest and during voluntary contraction.

Estimation of postsynaptic potentials in rat hypoglossal motoneurones: insights for human work.

Classical techniques for estimating postsynaptic potentials in motoneurones include spike-triggered averages of rectified surface and multiunit electromyographic recordings (SEMG and MU-EMG), as well as the compilation of peristimulus time histograms (PSTH) based on the discharge of single motor units (SMU). These techniques rely on the probability of spike occurrence in relation to the stimulus and can be contaminated by count- and synchronization-related errors, arising from post-spike refractoriness and the discharge statistics of motoneurones. On the other hand, since these probability-based techniques are easy to use and require only inexpensive equipment, it is very likely that they will continue to be used in clinical and laboratory settings for the foreseeable future. One aim of the present study was to develop a modification of these probability-based analyses in order to provide a better estimate of the initial phase of postsynaptic potentials. An additional aim was to combine probability-based analyses with frequency-based analyses to provide a more reliable estimate of later phases of postsynaptic potentials. To achieve these aims, we have injected simple as well as complex current transients into regularly discharging hypoglossal motoneurones recorded in vitro from rat brainstem slices. We examined the discharge output of these cells using both probability- and frequency-based analyses to identify which of the two represented the profile of the postsynaptic potential more closely. This protocol was designed to obtain PSTHs of the responses of single motor units to repeated application of the same afferent input. We have also simulated multiunit responses to afferent input by replacing the times of spike occurrence in individual trials with a representation of either an intramuscular or surface-recording single motor unit waveform and summing many of these trials to obtain either a simulated SEMG or MU-EMG. We found that in a regularly discharging motoneurone, the rising phase of an EPSP moves the occurrence of spikes forward and hence induces a substantial peak in all probability-based records. This peak is followed immediately by a period of reduced activity ('silent period') due to the phase advancement of spikes that were to occur at this period. Similarly, the falling phase of an IPSP delays spikes so that they occur during the rising phase of the IPSP. During the delay, the probability-based analyses display gaps and during the occurrence of the delayed spikes they generate peaks. We found that all the probability-based analyses (SEMG, MU-EMG and PSTH) can be made useful for illustrating the underlying initial PSP by a special use of the cumulative sum (CUSUM) calculation. We have illustrated that, in most cases, the CUSUM of probability-based analyses can overcome the delay- or advance-related (i.e. the count-related) errors of the classical methods associated with the first PSP only. The probability-based records also induce secondary and tertiary peaks and troughs due to synchronization of the spikes in relation to the stimulus (i.e. the synchronization-related errors) by the first PSP to occur at fixed times from the stimulus. Special CUSUM analyses cannot overcome these synchronization-related errors. Frequency-based analysis (PSFreq) of individual and summed trials gave comparable and often better indications of the underlying PSPs than the probability-based analyses. When used in combination, these analyses compliment each other so that a more accurate estimation of the underlying PSP is possible. Since the correct identification of the connections in the central nervous system is of utmost importance in order to understand the operation of the system, we suggest that as well as the using the special CUSUM approach on probability-based records, researchers should seriously consider the use of frequency-based analyses in their indirect estimation of stimulus-induced compound synaptic potentials in human motoneurones.

The effects of common input characteristics and discharge rate on synchronization in rat hypoglossal motoneurones.

Synchronous discharges between a pair of concurrently active motoneurones are thought to arise from the spike-triggering effects of synaptic inputs shared by the pair. Although there are a number of quantitative indices that have been developed to estimate the strength of this common input, there is still some debate as to whether motoneurone discharge rate affects the values of these indices. The aim of the present study was to test the effects of motoneurone discharge rate on these synchronization indices using known common inputs. To achieve this aim we elicited repetitive discharge in rat hypoglossal motoneurones by combining a suprathreshold injected current step with superimposed noise to mimic the synaptic drive likely to occur during physiological activation. The amplitude of the current step was varied in different trials to achieve discharge rates from 5 to 22 Hz. We first examined the effect of discharge rate on the spike-triggering efficacy of individual EPSPs. Motoneurones were more responsive to large EPSPs delivered at a low rate when their background discharge rate was relatively low and the probability of the EPSPs evoking an extra spike decreased with increasing discharge rate. However, the opposite dependence was found for small, high-frequency EPSPs. We then compared the discharge records obtained in several trials in which the same EPSP train was applied repeatedly to the same cell firing at different background discharge rates. The effect of this 'common input' on motoneurone discharge probability was determined by compiling cross-correlation histograms (CCHists) between the discharges of the same cell at different times. The common inputs induced synchronous discharge that gave rise to large central peaks in the CCHists. The relationship between the discharge rate and the level of synchronization changed depending on the synchronization indices used and the amplitude of the common EPSPs. When large EPSPs were used as the common input, the normalized probability of synchronous spikes declined as the discharge rate increased, regardless of the method of normalization used. In contrast, when the common input was composed of a large number of small EPSPs, similar to that likely to occur during physiological activation of motoneurones, different synchronization indices exhibited a positive, a negative or no dependence on the background discharge rate. Indices based on normalizing the number of synchronous spikes by either the number of discharges in the lower frequency train (E), or by the total number of discharges in both trains (S) showed no dependence on background discharge rate and therefore may be the most suitable for quantifying motoneurone synchrony over a range of background discharge rates.

Effects of common excitatory and inhibitory inputs on motoneuron synchronization.

We compared the effects of common excitatory and inhibitory inputs on motoneuron synchronization by simulating synaptic inputs with injected current transients. We elicited repetitive discharge in hypoglossal motoneurons recorded in slices of rat brain stem using a combination of a suprathreshold injected current step with superimposed noise to mimic the synaptic drive likely to occur during physiological activation. The effects of common inputs to motoneurons were simulated by the addition of a waveform composed of from 6 to 300 trains of current transients designed to mimic excitatory and/or inhibitory synaptic currents. We compared the discharge records obtained in several trials in which the same "common input" waveform was applied repeatedly in the presence of different background noise waveforms. The effects of the common input on motoneuron discharge probability and discharge rate were determined by compiling a cross-correlation histogram (CCHist) and a perispike frequencygram (PSFreq) between the discharges of the same cell at different times. Both excitatory and inhibitory common inputs induced synchronous discharge that was evident by a large central peak in the CCHist. The CCHists produced by common excitatory inputs were characterized by larger and narrower central peaks than those generated by common inhibitory inputs. The PSFreqs produced by common excitatory inputs indicated an increase in the discharge rate of motoneurons around time 0 that coincided with the narrow and large central peak in the CCHist. On the other hand, inhibitory inputs often generated very little, if any, change in the discharge rate around time 0 corresponding with the small and wide central peak in the CCHist. These results suggest that the CCHist indicates the effective strength of the net common input but not its sign. Although correlated changes in discharge rate are often quite different for net excitatory and inhibitory common input, except in some restricted conditions, the PSFreq analysis also cannot be used to unambiguously distinguish net excitation from net inhibition.

The role of the muscle spindles in human masseter.

In the limb muscles, the muscle spindles have been demonstrated to be important in the maintenance of static posture. This role is supported by the close proximity of the muscle spindles to motor units that develop small forces and are fatigue-resistant, and the greater effectiveness of the input from muscle spindle afferents onto the small motoneurons supplying these motor units. In masseter, input from the muscle spindles is more effective on the larger motoneurons. This suggests that the muscle spindles may be more important in masseter for the development of large, fast forces, rather than for the maintenance of static postures. Thus muscle spindles in masseter may be important in load compensation during chewing and for the development of powerful bite forces in aggressive or defensive situations.