ABSTRACT
The goal of the present study was to investigate the effects of ovariectomy and naproxen treatment on both femoral and mandibular bone mass and biomechanical competence. Sprague-Dawley rats were used and divided into five groups: baseline, sham ovariectomized, ovariectomized, sham ovariectomized + naproxen treatment, ovariectomized + naproxen treatment. Mandibles and femurs of the rats were extracted and bone mineral density of the extracted specimens were determined. The volumes and ash weights of the femurs and mandibles were estimated. Maximum loads of the femurs and mandibles were determined by using a three point bending test. Ovariectomy decreased bone mineral density of the femoral midshaft, however naproxen prevented this decrease. Neither ovariectomy nor naproxen treatment affected the BMD in the molar alveolar region. Maximum load was found significantly decreased in the femoral midshaft, however, naproxen treatment prevented this decrease. Maximum load of the molar alveolar region did not significantly change. Naproxen prevents the strength characteristics of femoral midshaft afforded by ovariectomy. However, neither ovariectomy nor naproxen has an effect on the molar alveolar region of the mandible.
El objetivo del presente estudio fue investigar los efectos de la ovariectomía y el tratamiento con naproxeno sobre la masa y la competencia biomecánica del fémur y hueso mandibular. Fueron utilizadas ratas Sprague-Dawley, la que se dividieron en cinco grupos: referencia, ovariectomizadas simuladas, ovariectomizadas, ovariectomizadas simuladas + tratamiento naproxeno, ovariectomizadas + tratamiento naproxeno. Las mandíbulas y los fémures de las ratas fueron extraídos y se determinó la densidad mineral ósea (DMO) de las muestras extraídas. Los volúmenes y pesos de la ceniza fueron estimados. Las cargas máximas de las mandíbulas y fémures se determinaron utilizando un ensayo de flexión de tres puntos. La ovariectomía disminuyó la densidad mineral ósea de la diáfisis media del fémur; sin embargo, el naproxeno impidió esta disminución. Ninguna ovariectomía sin tratamiento de naproxeno afectó a la DMO en la región alveolar molar. La carga máxima se encontró significativamente disminuida en las diáfisis femoral media, pero por el tratamiento de naproxeno no se produjo esta disminución. La carga máxima de la región alveolar molar no cambió en ninguna condición. El naproxeno previene los cambios de resistencia causados por la ovariectomía de la diáfisis media del fémur. Sin embargo, ni la ovariectomía ni el naproxeno tienen un efecto en la región alveolar molar de la mandíbula.
Subject(s)
Humans , Male , Infant, Newborn , Molar , Naproxen/therapeutic use , Femur , Rats, Sprague-Dawley/anatomy & histology , Rats, Sprague-Dawley/metabolismABSTRACT
An experimental comparative study on isolated guinea pig lungs has been undertaken to determine the probable beneficial effects of adding selenium to pulmonary preservation solutions in lung ischemia. The isolated lungs (n = 10 in each group) previously being perfused by oxygenated Krebs-Henseleit solution were put in normothermic ischemic conditions just after the infusion of 30 ml of pulmonary preservation solution (Euro-Collins in the control group, Euro-Collins plus selenium 10(-3) mol in the experiment group). After 3 hours of normothermic ischemia the lungs were reperfused with the same buffer for 20 minutes. Pulmonary artery pressures, tissue malondialdehyde levels, and adenosine deaminase levels of the perfusate were measured before and after the ischemic period and also at the end of reperfusion. An electron microscopic analysis was performed on the lung tissues at the end of the experimental procedure. According to our data, the addition of selenium to pulmonary preservation solution showed a significant protective effect regarding both ischemic and reperfusion injury.
