[Correction of psycho-emotional state by the biofeedback method in patients with metabolic syndrome].

Metabolic syndrome is one of the important clinical problems of medicine. The aim of this study was to demonstrate the efficacy of heart rate variability biofeedback method in correction of mental and emotional state in patients with metabolic syndrome. For this task was set to study the dynamics of indicators of mental and emotional stress by biofeedback based hardware-software complex "Amblyocor" in patients with metabolic syndrome. Course of heart rate variability biofeedback passed 10 patients with the metabolic syndrome. During biofeedback sessions conducted tests that assess psycho-emotional state by 5 characters. Data processing was performed using software package «Statistica 8.0¼ and showed a statistically significant decrease in indicators of mental and emotional stress, that demonstrates the effectiveness of biofeedback in the correction of mental and emotional stress in patients with metabolic syndrome.

Clinical and diagnostic features and treatment of giardiasis.

Giardia is the most common causes of protozoan diarrhea that lead to significant morbidity and mortality worldwide. Giardiasis can be cause of disturbance of host immune response. The treatment of Giardiasis is unsuccessful in some cases. The purpose of this study was to determine the clinical features and the content of secretory immunoglobulin A (sIgA) among adults and to evaluate efficiency of new plant preparation "Sausalin". The clinical studies were conducted in Karaganda Regional Infection Hospital (Kazakhstan). 250 patients with giardiasis were randomly assigned to receive sausalin at a dose 720 mg/day or ornidazole at 1500 mg/day. Clinical symptoms of giardisis and efficiency of treatment were evaluated. Protozoal clearance rate and clinical symptoms were assessed. Stool samples were collected from 40 patients and examined the content of sIgA. Our study found the prevalence of abdominal pain, dyspeptic syndrome and the symptoms of intoxication in patients with giardiasis. The increase the level of sIgA was detected, especially in females (88 mg/l). Sausalin was more effectiveness than ornidazole. After the treatment, the clearance rate of giardia (85.71% vs. 42.19%; P<0.05) and the clinical efficacy were significantly higher in the sausalin-treated group than in the ornidazole-treated group. The features of clinic manifestations of giardiasis were identified in population of Kazakhstan. Our data suggest the higher level of sIgA was significantly associated with features of clinic manifestations that the participant had. Treatment with sausalin was more effective than treatment with ornidazole. Further research is needed to explain the existence relationship between Giardia infection and host immune response.

[Activity of lysosomal hydrolases in various rabbit tissues in experimental atherosclerosis]. / Aktivnost' lizosomal'nykh gidrolaz v razlichnykh tkaniakh krolika pri éksperimental'nom ateroskleroze.

Activity of cathepsins D, B, C, H, L, acid lipase, acid cholesterol ester hydrolase, phospholipase A1, A2 and glucuronidase were studied in liver, small intestinal mucosa, intimal aortic cells, blood platelets and monocyte of rabbit after oral administration of cholesterol at daily dose of 300 mg/kg body weight within 100 days. Under this conditions distinct changes functional state of lysosomal systems were found in liver, monocyte and intima aortic cells. A possible mechanism of observed enzymological changes is discussed.

[Structure-activity status of the rat liver lysosomal apparatus upon the action of dithiocarbamate pesticides]. / Strukturno-funktsional'noe sostoianie lizosomnogo apparata pecheni krys pri deistvii pestitsidov ditiokarbamatnogo riada.

Total and non-sedimentable activity of cathepsins A, B, C and D in rat liver tissue as well as activity of these lysosomal proteinases in blood serum were studied during intragastric administration of tetramethyl thiuramdisulfide (TMTD), polymartsine and tsineb within 30 days at a dose of 1/20 LD50. The enzymatic activity alterations, which involved a decrease in total activity of lysosomal proteinases and an increase of the activity in non-sedimentable fraction and blood serum, were distinctly decreased in series: TMTD--polymartsine--tsineb. The data obtained suggest that structure-functional state of lysosomes was considerably altered in response to the effect of dithiocarbamate-containing pesticides.

[The content of cyclic nucleotides in an organ culture of normal and atherosclerosis-affected human aorta]. / Soderzhanie tsiklicheskikh nukleotidov v organnoi kul'ture normal'noi i porazhennoi aterosklerozom aorty cheloveka.

Cyclic AMP and cyclic GMP content was measured in intima media of unaffected and atherosclerotic areas of human aorta in a short-term organ culture. It was demonstrated that during short-term cultivation the content of both cyclic nucleotides in tissues is constant. The cyclic AMP content in fatty streaks and atherosclerotic plaques is significantly (2 to 7-fold) lower than in unaffected intima. The cyclic GMP level in atherosclerotic lesions is 1.5 to 3-fold higher than in normal. The content of both cyclic nucleotides in the media underlying fatty streaks and atherosclerotic plaques is the same as in the normal tissue. The obtained data indicate serious disorders in the system of cyclic nucleotides during atherosclerosis.

[Effect of silimarine on activity of lysosomal proteinases in the liver and kidneys during tetramethylthiuram disulfide administration]. / Vliianie silimarina na aktivnost' lizosomal'nykh proteinaz pecheni i pochek pri vvedenii tetrametiltiuramdisul'fida.

Total and nonsedimented activities of lysosomal proteinases were studied in rat liver and kidney tissues after administration of silimarine simultaneously with tetramethylthiuram disulfide (TMTD). Silimarine did not affect the pattern of alterations in total activity of lysosomal proteinases, caused by the TMTD treatment, but the nonsedimented activity of the enzymes studied was normalized. Silimarine appears to exhibit the membrane-stabilizing effect.

[Effect of glucagon on lysosomal proteinase activity after administration of tetramethylthiuram disulfide]. / Vliianie gliukagona na aktivnost' lizosomal'nykh proteinaz pri vvedenii tetrametiltiuramdisul'fida.

A pesticide tetramethylthiuram disulfide, administered per os at doses of 1/10 and 1/5 LD50 within 5 days, inhibited the activity of proteolytic system in liver and kidney lysosomes as well as impaired the lysosomal membrane structure in these tissues. Glucagon, injected simultaneously with the pesticide, prevented its inhibitory effect on lysosomal proteinases but did not affect the enzymatic activity in unsedimented fraction and in blood serum.