ABSTRACT
We analyzed cultures of 5 independent myoblast lines from human skeletal muscles. It was shown that the content of desmin-positive cells in cultures at early passages exceeds 90%. Typical morphofunctional signs of myogenic differentiation disturbances were identified and their dynamics was studied. Signs of alternative adipogenic and chondrogenic differentiation of cells were revealed. Based on these data, limitations for the use of myoblast cultures of certain passages for biomedical research and cell therapy were evaluated.
Subject(s)
Adipocytes/cytology , Chondrocytes/cytology , Myoblasts/cytology , Adipocytes/metabolism , Adult , Aggrecans/genetics , Aggrecans/metabolism , Biomarkers/metabolism , Cell Differentiation , Cell Proliferation , Cell- and Tissue-Based Therapy/statistics & numerical data , Chondrocytes/metabolism , Desmin/genetics , Desmin/metabolism , Female , Gene Expression , Humans , Male , Muscle Development/genetics , Muscle, Skeletal/cytology , Muscle, Skeletal/metabolism , Muscle, Skeletal/surgery , Myoblasts/metabolism , Primary Cell CultureABSTRACT
At present research we have demonstrated the link between introducing of low molecular weight RSH-antioxidants (N-acetylcysteine, glutathione) into serum-free medium composition, reactive oxygen species (ROS) generation and mouse myeloma SP2/0-SF cells proliferative activity. The presence of these compounds in the medium changed the pattern of ROS-activity in cells by concentration-dependent manner, and affected their proliferative characteristics. The optimal value of the proliferative activity was related to 0.2 mM for both thyols and not depended from the thyol-compound nature. Further increasing up from the found concentration optimum lead to growth inhibition with different expression for N-acetylcysteine and glutathione.
Subject(s)
Acetylcysteine/pharmacology , Cell Proliferation/drug effects , Free Radical Scavengers/pharmacology , Glutathione/pharmacology , Multiple Myeloma/metabolism , Reactive Oxygen Species/metabolism , Animals , Cell Line, Tumor , Mice , Multiple Myeloma/pathologyABSTRACT
Analysis of the main principles of classification and development of nutrient media used for culturing of human and animal cells in biology and medicine is presented. The key moments of induction and regulation of mitogenic cascades and their differences in cells of continuous lines, diploid cells, and primary cultures are discussed. Some variants of classification of nutrient media for various cell culture types are described. Peculiarities of composition and the prospects of using serum-free media are discussed. Practical results obtained by the authors in the development of diverse purpose nutrient media are presented.
