Assessing the potential biological activities of TiO2 and Cu, Ni and Cr doped TiO2 nanoparticles.

Nanoparticles are like magic bullets and nanomaterials exhibit appealing properties. Their size and morphology can be switched by dopants for certain biological activities. Nanoparticles in combination with certain drugs enhance the antibiotic effects and may be valuable in combating bacterial resistance. The antimicrobial potency of nanoparticles depends upon their ability to bind to the surface of microbial cell membranes resulting in modulation of basic cell functions such as respiration. We report herein the antibacterial, antifungal and antioxidant activities of pure TiO2 and TiO2 doped with 4% Cu, Ni and Cr. The performance of pure and doped nanoparticles has been compared with reference compounds. A comparison of the antifungal activities of the samples doped with TiO2 reveals that Cu-TiO2 exhibits improved performance against A. fumigatus but lower antifungal activity against Mucor sp. and F. solani. Cu-TiO2 and Ni-TiO2 showed good antibacterial action against B. bronchiseptica, while Cr-TiO2 nanoparticles displayed better activity against S. typhimurium as compared to pure TiO2. Moreover, pristine TiO2 and Ni-TiO2 nanoparticles were found to demonstrate maximum total antioxidant capacity.

Antioxidative-, Antimicrobial-, Enzyme Inhibition-, and Cytotoxicity-Based Fractionation and Isolation of Active Components from Monotheca buxifolia (Falc.) A. DC. Stem Extracts.

The current study elaborates the pharmacological potential of the methanolic extract and its fractions of the stems of Monotheca buxifolia based on thin-layer chromatography and column chromatography analyses, exploiting biological and phytochemical assays. The results suggest that bioassay-guided isolation and fractionation led to the accumulation of biologically active components in the most active fractions that resulted in the isolation of different compounds. Structural elucidation of the purified compounds was accomplished using spectroscopic one-dimensional (1H, 13C) and two-dimensional NMR (heteronuclear multiple quantum coherence, heteronuclear multiple bond coherence, and correlation spectroscopy) and spectrometric (electron ionization mass spectrometry and high-resolution electron ionization mass spectrometry) techniques. The n-hexane, CHCl3, and EtAOc fractions led to the isolation of lupeol from different fractions. 1-Triacontanol was also isolated from the n-hexane fraction, while benzoic acid, methyl benzoate, ursolic acid, and 3-hydroxybenzoic acid were obtained from the EtOAc fraction. The compounds depicted good-to-moderate total antioxidative potential and total reducing power activity and significant free-radical scavenging activity. All the compounds showed significant urease and lipase inhibitory activity with poor-to-moderate amylase inhibition. Significant zone of inhibition was observed against different bacterial strains by the isolated compounds. This work therefore states that bioassay-guided isolation plays a vital role in the isolation of biologically active constituents that can be exploited for drug development.

Anticancer and antibacterial potential of Rhus punjabensis and CuO nanoparticles.

The present study reports ecofriendly synthesis of CuO nanoparticles (NPs) using an extract of Rhus punjabensis as a reducing agent. NPs structural and composition analysis are evaluated by X-rays diffraction (XRD), Fourier transform infrared, Energy dispersive spectroscopy, Scanning electron microscopy, Transmission electron microscopy, and Thermal analysis. The NPs have pure single phase monoclinic geometry with spherical structure and high stability toward heat and with average particle size of about 36.6 and 31.27 nm calculated by XRD and SEM, respectively. NPs are tested for antibacterial, protein kinase (PK) inhibition, SRB cytotoxic, and NF-κB activities. Antibacterial activity is observed against B. subtilis and E. coli. Significant PK and SRB cytotoxic activity is observed with some NF-κB inhibition. NPs IC50 values against HL-60 and PC-3 prostate cancer cells are 1.82 ± 1.22 and 19.25 ± 1.55 µg/mL. The results encourage further studies for antibacterial and anticancer drug development of NPs using animal models.

In vitro antioxidant and anti-cancer activities and phytochemical analysis of Commelina benghalensis L. root extracts

Objective: To explore antioxidant potential, anti-cancer activity, and phytochemicals of Commelina benghalensis L. Methods: The roots of Commelina benghalensis were extracted in different solvents (methanol, ethanol, benzene, chloroform, n-hexane) with a range of polarity. Antioxidant activity was evaluated by reducing power assay, DPPH radical scavenging activity and phosphomolybdenum method, cytotoxicity by MTT assay, apoptotic and cell cycle analysis by flow cytometry, migratory and invasive potential by wound scratch assay and invasion assay, respectively, functional groups analysis by FT-IR spectroscopy and phytochemicals by aluminum chloride colorimetric and Folin-Ciocalteu methods. Results: The extracts showed worthy antioxidant potential. The chloroform extract demonstrated the most significant cytotoxic effect on MDA-MB-231 (breast cancer) cell line, induced apoptosis and reduced migratory and invasive potential of MDA-MB-231 cells. Methanol and ethanol extracts presented good yield of total phenolic and total flavonoid contents. The FTIR spectroscopic studies revealed different characteristic peak values with various functional compounds such as alkenes, alkanes, aliphatic amines, aromatics, alkyl halides, carboxylic acid, alcohols, ester, aldehydes and ketones. Conclusions: The results demonstrate the potential use of Commelina benghalensis as a good antioxidant with significant anti-cancer effect.

Phytochemical, in-vitro biological and chemo-preventive profiling of Arisaema jacquemontii Blume tuber extracts.

BACKGROUND: Arisaema jacquemontii is traditionally used in treatment of different diseases. In this study, phytochemical, in vitro biological and chemo-preventive screening of A. jacquemontii was carried out to explore its pharmacological potential. METHODS: The dried tuber of A. jacquemontii was extracted in 11 organic solvent mixture of different polarity. The extracts were screened for phytochemical assays (phenolics and flavonoids), antioxidants potential (free radical scavenging activity, total antioxidant activity, reducing power), biological activities (antibacterial, antifungal, cytotoxic, antileishmanial, protein kinase inhibition), and chemopreventive activities using different cell lines through standard protocols. RESULTS: Significant amount phenolic contents were determined in EtOH and MeOH extracts (210.3 ± 3.05 and 193.2 ± 3.15 µg GAE/mg, respectively). Maximum flavonoid content was determined in MeOH extract (22.4 ± 4.04 µg QE/mg). Noteworthy, DPPH scavenging activity was also recorded for MeOH extract (87.66%) followed by MeOH+EtOAc extract (85.11%). Considerable antioxidant capacity (7.8 ± 0.12 µg AAE/mg) and reducing power (3.1 ± 0.15 µg AAE/mg) was observed in extract of MeOH. The LC50 against brine shrimp and leishmanial parasite was found 9.01 and 12.87 µg/mL for n-Hex and CHCl3 extracts, respectively. The highest zone of inhibition against Streptomyces hyphae formation (12.5 ± 1.77 mm) by n-Hex extract. Growth zone of inhibition 13.8 ± 1.08 mm was recorded for EtOAc and MeOH extracts, respectively against Micrococcus luteus while 10.0 ± 0.11 mm for MeOH extract against Aspergillus flavus. In-vitro cytotoxic assay showed that n-Hex extract had higher cytotoxicity against DU-145 prostate cancer and HL-60 cancer cell lines. NF-kB and MTP potential showed 34.01 and 44.87 µg/mL for n-Hex and CHCl3 extracts, respectively in chemo-preventive potential. CONCLUSION: The study concludes that Arisaema jacquemontii bears significant phytochemical activity and pharmacological activities, this plant can be further explored for isolation of active component against a number of aliments.

Optimization of Ag2O nanostructures with strontium for biological and therapeutic potential.

Pristine- and strontium-doped Ag2O nanoparticles (NPs) were synthesized utilizing the symbolic co-precipitation method, in which sodium hydroxide was used as a precipitating agent. Various instrumentation methods were employed to get an inside view of the synthesized NPs. Powdered X-ray diffraction (PXRD) analysis revealed the existence of high crystallinity and small-sized NPs (an average diameter range of 35-48 nm). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) displayed spherical and circular disc-shaped morphology of the particles. Energy-dispersive X-ray spectroscopy (EDX) showed the purity of NPs. All the screened metal functionalized Ag2O NPs exhibited excellent cytotoxicity and antibacterial activities, moderate to good antioxidant and antifungal activities in comparison to Ag2O NPs. Furthermore, nanomaterials were evaluated for DNA interaction studies. The results illustrated that by increasing the concentration of dopant, i.e. strontium up to 5%, the binding affinity of the NPs effectively increased, hence causing the structural changes in DNA.

Report- Antioxidant, cytotoxicity, protein kinase inhibition and antibacterial activities of Fragaria x ananassa leaves.

Fragaria × ananassa leaves extracts prepared in different solvents were subject for antioxidative, cytotoxicity, protein kinase inhibition and antibacterial activities. The extracts showed varying activities depending upon solvent used for extraction. Combined effect of methanol and ethyl acetate showed maximum antioxidant and reducing power potential (207.65±6µg AAE/mg and 88.58±20µg AAE/mg, respectively). Maximum DPPH (2,2-diphenyl-1-picryl hydrazyl) free radical scavenging activity was calculated by when methanol: chloroform and acetate fractions were used (87.68% and 86.88% inhibition, respectively). Total phenolics varied from 186 to 1.91µg AAE/mg while total flavonoids also significantly varied among the extracts. The extracts also showed significant activities against brine shrimp larvae and bacterial strains tested. The study concludes that Fragaria × ananassa leaves can be a good source for isolation of active phytochemicals to be used in different industries.

Kinnow peel extract as a reducing and capping agent for the fabrication of silver NPs and their biological applications.

An effective approach used for the synthesis of silver nanoparticles (AgNPs) through green chemistry by using Kinnow peel extract as a reducing and capping agent is presented. Two different approaches, diluted and concentrated peel extracts, were used for the synthesis of AgNPs. Ultraviolet-visible spectroscopy exhibits characteristic absorption peaks at 425 and 400 nm for nanoparticles (NPs) synthesised by diluted and concentrated extracts, respectively. The X-ray diffraction analysis of nanofabricated silver exhibited a pure face centred cubic structure of 27.4 and 18.1 nm sizes calculated by using Scherrer equation. Scanning electron microscopy analysis showed a uniform morphology of synthesised NPs. Significant antioxidant, phytochemical and antibacterial assays show that both AgNPs can be effectively used in biomedical applications. Furthermore, the use of citrus peel for the synthesis of NPs can be an effective tool in waste management.

Appraisal of phytochemical and in vitro biological attributes of an unexplored folklore: Rhus Punjabensis Stewart.

BACKGROUND: The role of plants for discovery of therapeutic potential accentuates the need to know their biological attributes. The present study aims to comprehend the biological attributes of Rhus punjabensis, an unexplored traditional medicinal plant. METHODS: Leaf and stem extracts of R. punjabensis prepared in 11 different organic solvents are evaluated for multimode antioxidant potential, total phenolic and flavonoid contents were determined through colorimetric assays, HPLC-DAD analysis was carried out for quantification of various polyphenols in extracts. Brine shrimp lethality, SRB and MTT assays were used to elucidate plant's cytotoxic and antileishmanial potentials. Disc diffusion assay was used to elucidate the protein kinase inhibitory, antibacterial and antifungal spectrum. RESULTS: Ethanol + ethyl acetate yielded maximum extract recovery from leaf (6.11 ± 1.09% w/w), total phenolic content (80.5 ± 2.18 µg GAE/mg extract) and reducing power potential (165.4 ± 2.29 µg AAE/mg extract). Maximum flavonoid content (30.50 ± 1.11 µg QE/mg extract) and highest DPPH based free radical scavenging activity (IC50 11.4 ± 2.07) was exhibited by the methanol + chloroform leaf extract. The methanol extract showed maximum total antioxidant capacity (74.5 ± 2.25 µg AAE/mg DW), protein kinase inhibitory (12.5 ± 1.10 bald phenotype at 100 µg/disc) and antifungal (MIC = 25 µg/disc against Aspergillus flavus) potential. Reverse phase HPLC-DAD based quantification reveals presence of gallic acid, apigenin, rutin and catechin in various extracts. Brine shrimp lethality assay demonstrated most extracts as highly cytotoxic (LC50 < 50 µg/mL) whereas chloroform extract of leaf demonstrated maximuminhibition against human leukemia cell line (IC50 7.80 ± 0.01 µg/mL). A significant activity against leishmanial promastigotes was demonstrated by n-hexane leaf extract (IC50 = 15.78 ± 0.15 µg/mL). A better antibacterial activity,by the extracts, against Gram positive strains as compared to Gram negative was observed. CONCLUSIONS: Results recommend multiple-solvent system as a critical factor to sumptuous the biological prospective of R. punjabensis and propose it to be a useful natural hub for the discovery of novel antioxidant, anticancer, antileishmanial and antimicrobial agents.

ZnO nanostructure fabrication in different solvents transforms physio-chemical, biological and photodegradable properties.

Zinc oxide (ZnO) nanostructures are synthesized in various organic solvents (acetone, chloroform, ethyl acetate, ethanol and methanol) and water via coprecipitation process using zinc acetate as precursor. The resultant ZnO nanoparticles, nano rods and nano sheets are characterized by UV-vis spectrophotometric analysis, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transmission infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDX). The variable size and geometry of nanoparticles depend upon medium used for synthesis. The synthesized ZnO nanostructures exhibit minor to moderate antioxidative (DPPH based free radical scavenging activity, total antioxidative potential and total reducing power) response. Mild to moderate antibacterial and antifungal activities, excellent antileishmanial potential (IC50 up to 3.76), and good cytotoxic perspective (LD50 up to 49.4) is also observed by the synthesized ZnO NPs. The nanoparticles also exhibit moderate α-amylase inhibition response. Furthermore the nanostructures are evaluated for methylene blue photodegradation response within 60min time period. It is found that organic solvent alters shape, size and other physio-chemical properties of ZnO that ultimately modulate the biological, chemical, and environmental properties.