Treatment effects of Shilajit on aspirin-induced gastric lesions in rats.

The present study investigated the effects of Shilajit extract on aspirin-induced gastric lesions in rats. We evaluated macroscopic and histopathological lesions in the stomach, measured the activity of oxidative stress enzymes in gastric tissue homogenates, and assessed serum electrolytes and parameters of kidney and liver function. Forty-five male rats were allocated to five groups: Normal control, positive control, omeprazole treatment, Shilajit treatment, and Shilajit control. The treatment period lasted for four consecutive days. The size and number of gastric lesions were significantly reduced in the Shilajit and omeprazole groups compared to the positive control group, indicating a reduction in mucosal damage and the severity of edema and leukocyte infiltration in tissue sections. A significant increase was observed in the levels of all oxidative stress parameters, except malondialdehyde, in rats treated with Shilajit and omeprazole compared to those in the positive control group. The effect of the aqueous extract of Shilajit was comparable to that of omeprazole. These results indicated the protective effects of Shilajit against aspirin-induced gastric lesions.

Histopathological and immunohistochemical characterization of testicular T-cell lymphoma in a dog.

Canine lymphoma is the most common neoplasm of the hematopoietic system with the most frequently diagnosed malignancy. Also, dogs affected by T-cell lymphoma displayed a lower rate of complete chemotherapy response and have a high risk of death in the early stage of the disease. A 5-year-old male mixed dog was presented to the Small Animal Hospital, School of Veterinary Medicine, Shiraz University, Shiraz, Iran with a history of left testicle swelling for four months. The testicle had approximately 13.00×10.00 cm size with a greyish-white cut surface. Histopathologically, the testicular tissue was infiltrated by neoplastic cells as only a few degenerated seminiferous tubules have remained. The neoplastic cells were round to oval with pleomorphic nuclei and single or multiple prominent nucleoli. Immunohistochemical analysis revealed positive and negative immunoreactivity for CD3 and CD20, respectively. According to histopathological and immunohistochemical features, the tumor was concluded as a testicular T-cell lymphoma. It seems that recognition of lymphoma type could be helpful for clinicians therapeutic protocols.

Oxidative-antioxidative status and hepatic and renal factors following melatonin administration in castrated and intact dogs.

BACKGROUNDS: Melatonin has significant antioxidant and hepatoprotective effects in normal and oxidative stress conditions. The aim of the present study was to assess the effects of melatonin on antioxidant, hepatic, and renal factors in intact and castrated dogs. Twenty male mixed-breed adult dogs were aligned in an experimental randomized and controlled trial. The dogs were randomly divided into four equal groups: melatonin, castrated, castrated and melatonin, and control. They were treated with melatonin (0.3 mg/Kg, once daily, orally) immediately after the castration for 1 month and their blood samples were collected weekly from 2 days after treatment with melatonin. RESULTS: Treating castrated dogs with melatonin increased the level of glutathione peroxidase, superoxide dismutase, and catalase compared with that of the control and castrated groups. The malondialdehyde level increased significantly following castration. Melatonin treatment decreased malondialdehyde concentration in the castrated dogs. Castration increased the level of alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase significantly in comparison with that of the control group. Treating the castrated dogs with melatonin decreased significantly liver enzymes compared with those of the castrated dogs. Blood urea nitrogen and creatinine levels increased in the castrated dogs in comparison with that of the control group. CONCLUSIONS: The administration of melatonin in castrated dogs increased antioxidant activity and decreased oxidation products, compared with those of the castrated and untreated dogs, without adverse effects on liver enzymes and kidney function.

CONTEXTE: La mélatonine possède des effets anti-oxydants et hépato-protecteurs dans les conditions normales et dans les situations de stress oxydant. L'objectif de la présente étude est d'évaluer les effets de la mélatonine sur des facteurs anti-oxydants, hépatiques et rénaux chez des chiens intacts et castrés. Vingt chiens mâles adultes de race croisée ont été utilisés dans un essai expérimental randomisé avec groupe témoin. Les chiens ont été attribués au hasard à quatre groupes de même effectif : mélatonine, castré, castré et mélatonine, et témoin. Ils ont été traité par la mélatonine (0.3 mg/kg, une fois par jour, par voie orale) immédiatement après la castration, pendant un mois ; des échantillons sanguins ont été collectés une fois par semaine à partir du deuxième jour de traitement par la mélatonine. RÉSULTATS: Le traitment par mélatonine chez les chiens castrés augmente les niveaux de glutathion peroxydase, de superoxyde dismutase, et de catalase par comparaison avec les niveaux chez les groupes témoin et castré. Le niveau de malondialdéhyde augmente de façon significative après la castration. Le traitement par mélatonine diminue la concentration de malondialdéhyde chez les chiens castrés. La castration augmente significativement les niveaux de phosphatase alcaline, d'aspartate aminotransférase, et d'alanine aminotransférase par comparaison à ceux du groupe témoin. Le traitement des chiens castrés par la mélatonine diminue de façon significative les enzymes hépatiques par comparaison à ceux des chiens castrés. Les taux sanguins d'azote uréique et de créatinine augmentent chez les chiens castrés comparé à ceux des chiens témoins. CONCLUSIONS: L'administration de mélatonine à des chiens castrés augmente l'activité anti-oxydante, et diminue les produits d'oxydation, par comparaison avec les chiens du groupe castré et non traité, sans effets indésirables sur les enzymes hépatiques et sur la fonction rénale.

Changes in thyroid hormones, leptin, ghrelin and, galanin following oral melatonin administration in intact and castrated dogs: a preliminary study.

BACKGROUND: Melatonin regulates metabolism and metabolism related hormones in mammalians. Castration has some adverse effects on the metabolic hormones of dog. This study was conducted to determine the effects of oral melatonin administration on metabolic hormones, as well as to compare changes of these hormones after administration of melatonin in castrated and intact dogs. Twenty healthy mixed breed mature male dogs were divided randomly into four groups (n = 5): melatonin (3 mg/10 kg(, castrated, castrated and melatonin treated, and negative control. Blood sample was collected from jugular vein weekly for 1 month. RESULTS: T3 and T4 hormones had a significant decrease within 1 month following administration of melatonin. No significant change was observed in concentration of FT3 and FT4 hormones. Leptin and ghrelin hormones also had a significant decrease in this period. Leptin and ghrelin had a more significant decrease in "non-castrated and melatonin treated" group compared to "castrated and melatonin treated" group. Galanin had a significant decrease but this neurotransmitter had no significant change in "non-castrated and melatonin treated" group in comparison to "castrated and melatonin treated" group. CONCLUSIONS: It seems that daily administration of melatonin capsule in all dogs can probably decrease concentration of T3 and T4 hormones and balance other metabolic hormones following castration. METHODS: The dogs underwent castration, melatonin treatment and blood sampling.

Acute Transverse Myelitis in Children, Literature Review.

OBJECTIVE: Acute transverse myelitis (ATM) is a rare inflammatory demyelinating disorder characterized by relatively acute onset of motor, sensory, and autonomic dysfunction. Children comprise 20% of total cases of ATM. In this review, we described the current literature on childhood ATM, focusing on the epidemiology, pathogenesis, clinical presentation, approach to diagnosis, differential diagnosis, treatment and outcome in the pediatric population. MATERIALS &METHODS: We searched the related articles in electronic databases such as Scopus, EMBASE, Google Scholar, and PubMed. All study designs were included and the essential key words for searching were myelitis, acute transverse myelitis, childhood transverse myelitis, and acquired demyelinating syndromes. RESULTS: The related data focusing on the epidemiology, pathogenesis, clinical presentation, diagnostic approach and differential diagnosis, treatment and outcome of pediatric ATM were gathered and described. CONCLUSION: ATM is a heterogeneous disorder in children with a broad spectrum of clinical presentation, etiology, and outcome. It may be the first presentation of relapsing acquired demyelinating syndromes and also must be distinguished from compressive and noninflamatory myelopathies. Correct diagnosis is crucial for treatment and prognosis.

Relationship between Plasma Levels of Zinc and Clinical Course of Pneumonia.

BACKGROUND: Pneumonia is a common disease and is more prevalent among children and the elderly. Zinc (Zn) is an essential substance for the human body and plays an important role in regulating the immune system. Studies have shown a possible relation between the Zn plasma levels and pneumonia. MATERIALS AND METHODS: In a cross-sectional study, 100 patients with pneumonia, who were referred to the Educational-Medical Centers of Tabriz University of Medical Sciences, were included in the study. The plasma levels of Zn of all patients were measured. The patients were divided into two groups of normal and low plasma levels of Zn. The severity and clinical course of pneumonia, including the durations of fever, tachycardia, and tachypnea were evaluated and compared between the two groups. RESULTS: The plasma levels of Zn were normal in 56 patients and low in 44 patients. The mean duration of fever, tachycardia, and tachypnea in the group with normal plasma levels of Zn were 1.58±0.68, 2.04±0.81, and 2.78±0.84 days, respectively; and those in the group with decreased Zn plasma levels were 1.72±0.70, 2.18±0.90, and 2.97±0.91 days, respectively. There were no statistically significant differences between the two groups (P>0.05). However, the incidence of severe pneumonia was significantly less in the group with normal Zn plasma levels (P=0.001). CONCLUSION: Based on the findings of the present study, there was no statistically significant relationship between the plasma levels of Zn and the clinical course of pneumonia. However, Zn lowered the incidence of severe pneumonia.

Gastroscopic Study of Meloxicam, Tramadol, and Their Combined Administration on the Development of Gastric Injuries in Dogs.

Opioid and non-steroid anti-inflammatory drugs (NSAIDs) are commonly used to manage post-operative pain that may be chronically extended. Although NSAIDs have been approved for their analgesic effects in canine, they are mostly known to be associated with side effects, particularly gastric ulcers. In the present study, we evaluated short-term co-administration of meloxicam and tramadol to see if this could induce more gastric ulcers than that observed when using these drugs individually. Twenty adult mixed domestic dogs weighing 10 to 20 kg of both sexes, were randomly assigned to four groups of five dogs. In the control group, placebo was administered orally (twice a day), whereas the test groups received tramadol (per OS) twice a day, meloxicam (per OS) daily, and a combination of tramadol (twice a day) and meloxicam (daily) for ten days, respectively. The animals were evaluated for gastric injuries on days 0, 6, 11, 16 and 21 by endoscopy under general anesthesia. Clinical signs of all animals including fecal consistency, appetite, mental and hydration statuses, tempreture, heart rate and respiratory rate were evaluated daily. Based on our results, there was no significant difference between the experimental and control groups. In conclusion, our study demonstrated that a 10-day oral coadministration of tramadol and meloxicam had no deleterious effects on general health status and gastric mucosa in dogs.

Diagnostic Value of the Urine Mucus Test in Childhood Masturbation among Children below 12 Years of Age: A Cross-Sectional Study from Iran.

BACKGROUND: Childhood masturbation (CM) is considered a variant of normal sexual behavior; however, it is commonly misdiagnosed as epilepsy and movement disorders. As the first study from Iran, we analyzed a large population of infants and children with CM in a case-control study and evaluated the value of mucus in urine analysis as an alternative diagnostic tool for CM. METHODS: A total of 623 children referred to the Pediatric Neurology Clinic of Imam Khomeini Hospital for an evaluation of seizure or movement disorders were studied between 2008 and 2011. Totally, 359 children were found to have masturbatory behaviors (Group A) and the rest (264) were assigned to Group B. CM was diagnosed by direct observation. Collected data comprised demographic characteristics, clinical and neurodevelopmental examinations, laboratory findings (particularly urine analysis), and electrocardiography. RESULTS: The age of the children with CM was below 12 years old, and the girl-to-boy ratio was 7:1. Mucus in urine was positive in 357 (99.44%) children in Group A and 22 (8.3%) in Group B (P<0.001). A significant correlation was found between the presence of mucus in urine and masturbatory behaviors (P<0.001). CONCLUSION: Our findings suggest that the presence of mucus in urine can be used as an alternative laboratory test in children with CM below 12 years old and even in infants (≤24 months old). Further studies are needed to confirm the results.

Refractory Convulsive Status Epilepticus in Children: Etiology, Associated Risk Factors and Outcome.

OBJECTIVE: Refractory status epilepticus (RSE) is a life-threatening disease in children wherein the patient's convulsive seizures do not respond to adequate initial anticonvulsants. RSE is associated with high rate of mortality and morbidity. This study was aimed to survey the risk factors leading status epilepticus (SE) to RSE in children, and their early outcome. MATERIALS & METHODS: Patients with SE hospitalized in Tabriz Children's Hospital, Iran were studied during the years 2007 and 2008 with regard to their clinical profile, etiology, the treatment methods available to them and their outcome upon release from the hospital. RESULTS: Among 132 patients with SE, 53 patients (40.15%) suffered from RSE. Acute symptomatic etiology was a risk factor responsible for developing RSE in the patient (P=0.004). Encephalitis was the most common etiology of acute symptomatic SE. There was no significant relationship observed between RSE and the patients' age, gender, date of initial drug intake and type of seizure. The mortality rate was 8.3% and a new neurological deficit occurred in 25.7% of cases. None of RSE with encephalitis returned to the baseline status. Mortality and morbidity rates were significantly higher in children with RSE than in those with SE (P=0.006). CONCLUSION: Etiology of SE significantly influenced prognosis of it with significant incidence of RSE in acute symptomatic group. Because acute neurological insult such as encephalitis and meningitis are common causes of RSE in children, properly management of them is necessary to avoid permanent brain damage.

Epilepsy as a rare neurologic manifestation of oculodentodigitalis dysplasia.

Oculodentodigitalis dysplasia (ODDD) is an extremely rare inherited disorder involving the development of the face, eyes, teeth and limbs. In addition, some patients develop neurological problems mostly a spastic paraparesis associated with white matter abnormalities on magnetic resonance imaging. This report describes a patient with epilepsy, a rare neurologic manifestation of this syndrome.